{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:56:41Z","timestamp":1786978601704,"version":"build-2736575974"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,5,31]],"date-time":"2021-05-31T00:00:00Z","timestamp":1622419200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007860","name":"Ilam University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007860","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Brucellosis is a zoonotic disease causes by\n                    <jats:italic>Brucella<\/jats:italic>\n                    bacteria. So far, there is not any efficient treatment for this infectious disease in animals, although subunit vaccines can be a safe alternative. To this aim, we have designed a new chimeric OMP25\u2010OMP31 antigen formulated in Chitosan nanoparticles and studied its protective efficiency in vivo. OMP25\u2013OMP31 was produced using spliced overlap extension by polymerase chain reaction and the 3D protein structure and antigenic ability were predicted using computational tools. In addition, the humoural and cellular immune responses were measured by ELISA in six different experimental groups. The immune response showed chimeric rOMP25\u2013OMP31 antigen\u2010induced higher titers of IFN\u2010\u03b3 and TNF\u2010\u03b1 cytokines, while the lowest amount of IL\u20104 was dedicated to itself. Also, rOMP25\u2013OMP31 stimulated higher titer of IgG than individual injection of rOMP25 and rOMP31 treatments and the cell proliferation assay demonstrated the vaccination with rOMP25\u2013OMP31 elicits a vigorous antigen\u2010specific cell proliferative. In addition, the challenge experiment showed immunised mic stimulated a higher level of protection than negative controls. Overall, the results of rOMP25\u2013OMP31 could be promising for considering chimeric constructs as a feasible vaccine candidate for further investigations against brucellosis.\n                  <\/jats:p>","DOI":"10.1002\/vms3.537","type":"journal-article","created":{"date-parts":[[2021,5,31]],"date-time":"2021-05-31T07:21:13Z","timestamp":1622445673000},"page":"2008-2014","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Evaluation of the immunogenicity and efficacy of a chimeric OMP25\u2013OMP31 antigen in BALB\/c mice"],"prefix":"10.1002","volume":"7","author":[{"given":"Yahya","family":"Mohammadi","sequence":"first","affiliation":[{"name":"Department of Animal Science, Faculty of agriculture Ilam University Ilam Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,5,31]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/20905068.2019.1592890"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/apm.12778"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.12.012"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.10.6537-6546.2005"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2012.09.039"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.05080-11"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2013.07.041"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00527-14"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2013.04.003"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-8-4"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(02)00075-8"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(00)01350-2"},{"key":"e_1_2_9_14_1","first-page":"349","article-title":"A review of Brucellosis in Iran: Epidemiology, Risk Factors, Diagnosis, Control, and Prevention","volume":"21","author":"Golshani M.","year":"2017","journal-title":"Iranian Biomedical Journal"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2015.01.009"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/femspd\/ftx094"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.18869\/modares.iem.2.4.32"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00689-17"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.10.6541-6544.2001"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01151-08"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/1297-9716-45-61"},{"key":"e_1_2_9_22_1","first-page":"499","article-title":"Cloning, molecular analysis and epitopics prediction of a new chaperone GroEL Brucella melitensis antigen","volume":"18","author":"Sekhavati M. 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