{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T19:55:34Z","timestamp":1766087734684,"version":"3.40.5"},"reference-count":31,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,7,10]],"date-time":"2021-07-10T00:00:00Z","timestamp":1625875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Prevalence of brucellosis and MLVA genotyping in animals and humans, isolated from different regions of Pakistan was performed. Animals having history of brucellosis from the field and local farms of Bannu, Mardan, Peshawar, Swat, Lahore and Islamabad were selected for blood collection. Humans that work with them were also selected for sampling in this study. Total of 600 samples were taken from cattle and humans and subjected to Rose Bengal plate Test (RBPT) for the initial screening of positive samples. Designed primers of <jats:italic>B.abortus<\/jats:italic> for cattle and <jats:italic>B.melitensis<\/jats:italic> for humans were utilised to perform PCR. Culturing and isolation was carried to further to perform MLVA genotyping assay through the selection of two panels of primer markers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>RBPT showed more number of cases of brucellosis in animals and humans compared to the PCR findings. Genotyping findings based upon MLVA\u201015 set of markers demonstrated that the isolated strains of <jats:italic>B.abortus<\/jats:italic> fall in the same clade with strain A1, P8 and A2 from Pakistan and also similar with BCCN#02\u201045 strain from India. On the other hand, <jats:italic>B.melitensis<\/jats:italic> isolated from different districts of Pakistan shared the same clade with BwIM\u2010AFG 63, BwIM\u2010AFG 44 strains from Afghanistan and BwIM IRN 37 strain from Iran. Selected VNTR alleles were sequenced for calibration purposes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>It is concluded that <jats:italic>B<\/jats:italic><jats:italic>rucella<\/jats:italic> is prevalent in animals and humans in studied districts of Pakistan. Moreover, A1, P8, BwIM\u2010AFG 63, BwIM\u2010AFG 44 and A2 were found the common genotypes in Pakistan.<\/jats:p><\/jats:sec>","DOI":"10.1002\/vms3.550","type":"journal-article","created":{"date-parts":[[2021,7,10]],"date-time":"2021-07-10T11:10:41Z","timestamp":1625915441000},"page":"1688-1695","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Genotyping of <i>Brucella<\/i> strains isolated from humans and cattle of different geographical regions of Pakistan using MLVA\u201015"],"prefix":"10.1002","volume":"7","author":[{"given":"Raheela","family":"Akhtar","sequence":"first","affiliation":[{"name":"Department of Pathology University of Veterinary and Animal Sciences  Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7930-7782","authenticated-orcid":false,"given":"Muhammad Muddassir","family":"Ali","sequence":"additional","affiliation":[{"name":"Institute 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