{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T08:49:20Z","timestamp":1781945360029,"version":"3.54.5"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,11,11]],"date-time":"2021-11-11T00:00:00Z","timestamp":1636588800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Berberine (BBR) is always used in combination with florfenicol for treating avian in China.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study aims to investigate the effects of BBR on the pharmacokinetics of florfenicol in broilers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Male broilers were randomly divided into the control group and the BBR group (BG). Note that 50\u00a0mg\/kg BBR or sterile water was orally administrated to broilers. On the 8th day, florfenicol [30\u00a0mg\/kg body weight (BW)] was orally administered to broilers in both groups. The plasma concentrations of florfenicol were determined by ultra\u2010high\u2010performance liquid chromatography (UHPLC). The levels of cytochrome P450 (CYP) 3A37, multidrug resistance 1 (MDR1), and chicken xenobiotic\u2010sensing orphan nuclear receptor (CXR) mRNA expression in the liver and jejunum were determined by the real\u2010time PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results showed that the C<jats:sub>max<\/jats:sub>, t<jats:sub>1\/2z<\/jats:sub>, MRT<jats:sub>(0\u2010\u221e)<\/jats:sub>, and AUC<jats:sub>(0\u2010\u221e)<\/jats:sub> of florfenicol in BG were significantly increased (by 55.71%, 28.32%, 35.19%, and 55.62%, respectively), while the T<jats:sub>max<\/jats:sub> and CLz\/F of florfenicol were significantly decreased (by 52.13% and 35.82%, respectively). In BG, the levels of CYP3A37, MDR1, and CXR mRNA expression in the liver were significantly decreased to 0.72\u2010fold, 0.67\u2010fold, and 0.59\u2010fold, respectively, and the corresponding mRNA expression in the jejunum were significantly decreased to 0.66\u2010fold, 0.55\u2010fold, and 0.64\u2010fold levels, respectively, relative to their levels in the control group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>BBR altered the pharmacokinetics of florfenicol, probably related to its inhibition of CYP3A37, MDR1, and CXR mRNA expression in the jejunum and liver.<\/jats:p><\/jats:sec>","DOI":"10.1002\/vms3.660","type":"journal-article","created":{"date-parts":[[2021,11,12]],"date-time":"2021-11-12T07:42:41Z","timestamp":1636702961000},"page":"619-625","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Effects of berberine on the pharmacokinetics of florfenicol and levels of cytochrome P450 3A37, multidrug resistance 1, and chicken xenobiotic\u2010sensing orphan nuclear receptor mRNA expression in broilers"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1111-3580","authenticated-orcid":false,"given":"Sicong","family":"Li","sequence":"first","affiliation":[{"name":"Sichuan Animal Science Academy  Chengdu P. 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Y.(2012). Priliminary interaction study of florfenicol with polyether ionophore coccidiostats in broiler chichensnary interaction study of florfenicol with polyether ionophore coccidiostats in broiler chickens. Nanjing Agricultural University Nanjing. 96pp. (In Chinese)"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvp.12020"},{"issue":"1","key":"e_1_2_11_30_1","first-page":"9","article-title":"The roles of cytochrome P450 and P\u2010glycoprotein in the pharmacokinetics of florfenicol in chickens","volume":"19","author":"Wang G. Y.","year":"2018","journal-title":"Iranian Journal of Veterinary Research"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075689"},{"issue":"5","key":"e_1_2_11_32_1","first-page":"565","article-title":"Effects of berberine chloride and coadministration with cyclosporin on CPY3A1 in rat liver and small intestine","volume":"9","author":"Xin H. 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