{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T13:54:19Z","timestamp":1781877259807,"version":"3.54.5"},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,8,23]],"date-time":"2024-08-23T00:00:00Z","timestamp":1724371200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,8,23]],"date-time":"2024-08-23T00:00:00Z","timestamp":1724371200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    A 12\u2010year\u2010old male domestic cat with multiple subcutaneous mast cell tumours (MCTs) presented with a 2\u2010week history of pruritus and raw\/bleeding skin from self\u2010trauma at Kagoshima University Veterinary Teaching Hospital. Polymerase chain reaction (PCR) and histopathological analyses revealed intertumoral heterogeneity among tumour locations based on the mutation status of\n                    <jats:italic>KIT<\/jats:italic>\n                    . In addition, the expression pattern of KIT was characterized. After failed treatment with vinblastine (2.0\u20132.2\u00a0mg\/m\n                    <jats:sup>2<\/jats:sup>\n                    , intravenous administration, two doses in total) or nimustine (25\u00a0mg\/m\n                    <jats:sup>2<\/jats:sup>\n                    , intravenous administration, two doses in total), toceranib (2.2\u20132.6\u00a0mg\/kg, orally administered, every other day) was administered to treat recurrent MCTs harbouring the\n                    <jats:italic>KIT<\/jats:italic>\n                    exon eight internal tandem duplication mutation, achieving a complete response. However, toceranib resistance developed 2 months after treatment initiation. Subsequent PCR analysis was conducted to identify the mutational status of\n                    <jats:italic>KIT<\/jats:italic>\n                    in each MCT and to detect the presence of secondary mutations associated with the acquisition of toceranib resistance. Secondary\n                    <jats:italic>KIT<\/jats:italic>\n                    mutations (c.998G&gt;C and c.2383G&gt;C), which were not initially detected in tumour cells at diagnosis, were identified after the development of resistance to toceranib. This indicates that the tumour cells in feline MCTs in the same case have diverse characteristics. Our findings encourage further investigation into the development of therapeutic strategies for feline MCTs, particularly focusing on the heterogeneous nature of\n                    <jats:italic>KIT<\/jats:italic>\n                    \/KIT and overcoming acquired resistance to toceranib.\n                  <\/jats:p>","DOI":"10.1002\/vms3.70003","type":"journal-article","created":{"date-parts":[[2024,8,23]],"date-time":"2024-08-23T08:31:32Z","timestamp":1724401892000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A case report of feline mast cell tumour with intertumoral heterogeneity: Identification of secondary mutations c.998G&gt;C and c.2383G&gt;C in KIT after resistance to toceranib"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5035-913X","authenticated-orcid":false,"given":"Hiroyuki","family":"Tani","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Radiology Department of Veterinary Medicine College of Bioresource Sciences Nihon University  Fujisawa Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7511-0767","authenticated-orcid":false,"given":"Tatsuro","family":"Hifumi","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Histopathology Joint Faculty of Veterinary Medicine Kagoshima University  Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keita","family":"Ito","sequence":"additional","affiliation":[{"name":"Canine\u2010Lab. Inc.  Koganei Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohide","family":"Kuramoto","sequence":"additional","affiliation":[{"name":"Kagoshima University Veterinary Teaching Hospital Joint Faculty of Veterinary Medicine Kagoshima University  Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriaki","family":"Miyoshi","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Histopathology Joint Faculty of Veterinary Medicine Kagoshima University  Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Fujiki","sequence":"additional","affiliation":[{"name":"Kagoshima University Veterinary Teaching Hospital Joint Faculty of Veterinary Medicine Kagoshima University  Kagoshima Japan"},{"name":"Laboratory of Veterinary Surgery Joint Faculty of Veterinary Medicine Kagoshima University  Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohiro","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Radiology Department of Veterinary Medicine College of Bioresource Sciences Nihon University  Fujisawa Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,23]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/1098612X17695898"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092\u20108674(95)90455\u20107"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010017\u201001174\u20103"},{"key":"e_1_2_14_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12625"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10738"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917\u2010020\u201002284\u20109"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tranon.2017.09.007"},{"key":"e_1_2_14_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010022\u201019089\u20105"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102141.2009.07926.x"},{"key":"e_1_2_14_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvp.12816"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/vco.12677"},{"key":"e_1_2_14_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476\u20105829.2011.00275.x"},{"key":"e_1_2_14_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2020.03.007"},{"key":"e_1_2_14_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/030098589102800506"},{"key":"e_1_2_14_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2017.05.004"},{"key":"e_1_2_14_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/vco.12032"},{"key":"e_1_2_14_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985813476064"},{"key":"e_1_2_14_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416\u2010019\u20100389\u20106"},{"key":"e_1_2_14_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917\u2010021\u201002864\u20103"},{"key":"e_1_2_14_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/vco.12525"},{"key":"e_1_2_14_22_1","doi-asserted-by":"publisher","DOI":"10.1593\/neo.05622"},{"key":"e_1_2_14_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.05.055"}],"container-title":["Veterinary Medicine and Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.70003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/vms3.70003","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.70003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T13:17:55Z","timestamp":1781875075000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/vms3.70003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,23]]},"references-count":22,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["10.1002\/vms3.70003"],"URL":"https:\/\/doi.org\/10.1002\/vms3.70003","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/VMS3.70003\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70003\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70003\/v1\/review3","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70003\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70003\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70003\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70003\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70003\/v1\/review2","asserted-by":"object"}]},"ISSN":["2053-1095","2053-1095"],"issn-type":[{"value":"2053-1095","type":"print"},{"value":"2053-1095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,23]]},"assertion":[{"value":"2024-04-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70003"}}