{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:56:00Z","timestamp":1786978560081,"version":"build-2736575974"},"reference-count":20,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,8,29]],"date-time":"2024-08-29T00:00:00Z","timestamp":1724889600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,8,29]],"date-time":"2024-08-29T00:00:00Z","timestamp":1724889600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21K12328"],"award-info":[{"award-number":["21K12328"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Infections by gastrointestinal parasites are found in a variety of animals worldwide. For the diagnosis of such infections, the flotation method is commonly used to detect parasitic microorganisms, such as oocysts or eggs, in feces. Instead of adding a flotation solution after the final centrifugation step and using a cover slip to collect the parasites, the method using a wire loop for the recovery of the organisms has been reported as one of alternative methods. However, the recovery rates of microorganisms from the flotation method have not been analysed. In the present study, the utility of a flotation method with the use of a wire loop of 8\u00a0mm in diameter (the loop method) was evaluated using different numbers of\n                    <jats:italic>E. tenella<\/jats:italic>\n                    oocysts and\n                    <jats:italic>Heterakis gallinarum<\/jats:italic>\n                    eggs, and chicken fecal samples collected at the farms. Consequently, we found that the oocysts and eggs in tubes could be collected at a ratio of 2.00 to 3.08. Thus, our results indicate that the loop method is a simple and time saving method, implicating the application for the estimated OPG\/ EPG (Oocysts\/Eggs per gram) of the samples.\n                  <\/jats:p>","DOI":"10.1002\/vms3.70007","type":"journal-article","created":{"date-parts":[[2024,8,29]],"date-time":"2024-08-29T09:29:08Z","timestamp":1724923748000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Evaluation of the detection method by a flotation method using a wire loop for gastrointestinal parasites"],"prefix":"10.1002","volume":"10","author":[{"given":"Aruto","family":"Takano","sequence":"first","affiliation":[{"name":"Graduate School of Veterinary Medical Sciences Osaka Metropolitan University  Osaka Japan"},{"name":"Tri\u2010Ace Co.,Ltd.  Nihonmatsu Fukushima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daikichi","family":"Morinaga","sequence":"additional","affiliation":[{"name":"Graduate School of Veterinary Medical Sciences Osaka Metropolitan University  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isao","family":"Teramoto","sequence":"additional","affiliation":[{"name":"Graduate School of Veterinary Medical Sciences Osaka Metropolitan University  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshimitsu","family":"Hatabu","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Physiology Graduate School of Environmental Life Natural Science and Technology Okayama University  Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasutoshi","family":"Kido","sequence":"additional","affiliation":[{"name":"Departments of Virology and Parasitology Graduate School of Medicine Osaka Metropolitan University  Osaka Japan"},{"name":"Osaka International Research Center for Infectious Diseases Osaka Metropolitan University  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Kaneko","sequence":"additional","affiliation":[{"name":"Departments of Virology and Parasitology Graduate School of Medicine Osaka Metropolitan University  Osaka Japan"},{"name":"Osaka International Research Center for Infectious Diseases Osaka Metropolitan University  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Hatta","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Tropical Medicine Kitasato University School of Medicine  Sagamihara Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naotoshi","family":"Tsuji","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Tropical Medicine Kitasato University School of Medicine  Sagamihara Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigehiko","family":"Uni","sequence":"additional","affiliation":[{"name":"Faculty of Science Institute of Biological Sciences Universiti Malaya  Kuala Lumpur Malaysia"},{"name":"Faculty of Health and Welfare Studies Department of Health Sports and Nutrition Kobe Women's University  Hyogo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazumi","family":"Sasai","sequence":"additional","affiliation":[{"name":"Graduate School of Veterinary Medical Sciences Osaka Metropolitan University  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiromitsu","family":"Katoh","sequence":"additional","affiliation":[{"name":"Graduate School of Veterinary Medical Sciences Osaka Metropolitan University  Osaka Japan"},{"name":"Tri\u2010Ace Co.,Ltd.  Nihonmatsu Fukushima Japan"},{"name":"Department of Veterinary Clinical Sciences College of Veterinary Medicine University of the Philippines Los Ba\u00f1os  College Laguna Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2191-8919","authenticated-orcid":false,"given":"Makoto","family":"Matsubayashi","sequence":"additional","affiliation":[{"name":"Graduate School of Veterinary Medical Sciences Osaka Metropolitan University  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-010-9582-6"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.7.6.524-528.1978"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12639-021-01377-y"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2021.109463"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.2478\/s11686-019-00120-1"},{"key":"e_1_2_9_7_1","first-page":"15","article-title":"Comparison of common fecal flotation techniques for the recovery of parasite eggs and oocysts","volume":"6","author":"Dryden M. 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