{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T06:28:11Z","timestamp":1788935291007,"version":"build-2803163510"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,10,5]],"date-time":"2024-10-05T00:00:00Z","timestamp":1728086400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Rickettsial infections are often neglected and poorly recognized by physicians in many tropical and subtropical regions. Despite a number of recent reports describing rickettsial diseases in new locations and the discovery of new rickettsiae, medical science and research have largely neglected the diagnosis and antimicrobial treatment of rickettsial infections in subtropical and tropical areas; thus, much remains to be discovered. This study aimed to detect and characterize spotted fever group (SFG) rickettsiae in ixodid ticks infesting domestic ruminants in Khartoum State.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Polymerase chain reaction targeting both genes that encode for citrate synthase (\n                      <jats:italic>gltA<\/jats:italic>\n                      ) and outer membrane protein (\n                      <jats:italic>ompA<\/jats:italic>\n                      ) was performed for the presence of SFG rickettsia followed by sequence and phylogenetic analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Of the 202 ticks examined for the presence of SFG rickettsia,\n                      <jats:italic>gltA<\/jats:italic>\n                      gene was detected in 4 samples (2%). Furthermore,\n                      <jats:italic>gltA<\/jats:italic>\n                      \u2010positive samples were used to amplify the\n                      <jats:italic>ompA<\/jats:italic>\n                      gene, in which only two samples yielded positive results. Sequence and phylogenetic analysis of the positive samples revealed four different species of SFG rickettsiae:\n                      <jats:italic>Rickettsia aeschlimannii<\/jats:italic>\n                      ,\n                      <jats:italic>Rickettsia rhipicephali<\/jats:italic>\n                      ,\n                      <jats:italic>Rickettsia massiliae<\/jats:italic>\n                      and\n                      <jats:italic>Rickettsia raoultii<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These results indicated the presence of SFG rickettsia in Sudanese ticks. This also indicates that humans have an opportunity to acquire these infections. It is important to keep in mind the need for careful consideration of rickettsial infections in individuals with a fever of unknown origin.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70014","type":"journal-article","created":{"date-parts":[[2024,10,5]],"date-time":"2024-10-05T07:55:09Z","timestamp":1728114909000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["First molecular diagnosis of the human pathogen\n                    <i>Rickettsia raoultii<\/i>\n                    and other spotted fever group rickettsiae in Sudanese ixodid ticks from domestic ruminants"],"prefix":"10.1002","volume":"10","author":[{"given":"Nagwa","family":"Eisawi","sequence":"first","affiliation":[{"name":"Central Laboratory Ministry of Higher Education and Scientific Research  Khartoum Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jabbar","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Division of Veterinary Infection Biology and Immunology Research Center Borstel  Borstel Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed A.","family":"Bakheit","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine University of Khartoum  Khartoum North Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dina A.","family":"Hassan","sequence":"additional","affiliation":[{"name":"Central Laboratory Ministry of Higher Education and Scientific Research  Khartoum Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6661-2988","authenticated-orcid":false,"given":"Mohammed O.","family":"Hussien","sequence":"additional","affiliation":[{"name":"Central Laboratory Ministry of Higher Education and Scientific Research  Khartoum Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3951-9315","authenticated-orcid":false,"given":"Abdel Rahim M.","family":"El Hussein","sequence":"additional","affiliation":[{"name":"Central Laboratory Ministry of Higher Education and Scientific Research  Khartoum Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,5]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2010.0241"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-018-5848-0"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-47-2-548"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1374.073"},{"key":"e_1_2_11_6_1","unstructured":"Burgdorfer W. 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