{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:32:14Z","timestamp":1792801934447,"version":"4.5.0"},"reference-count":51,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,9,17]],"date-time":"2024-09-17T00:00:00Z","timestamp":1726531200000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T00:00:00Z","timestamp":1725148800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Ticks are vectors of pathogens that affect the health of animals and humans. With the constant trade of livestock across borders, there is the risk of new tick species invasion accompanied by the spread of infectious tick\u2010borne pathogens.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>This study sought to determine the diversity of tick species within abattoirs and a slaughter slab as well as identify the pathogens carried by these ticks.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The ticks were collected from slaughtered cattle, identified and screened for pathogens using PCR and sequencing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 371 ticks were collected from slaughtered cattle across the three sampling sites: Kumasi abattoir (288, 77.63%), Akwatia Line slaughter slab (52, 14.02%) and Suame abattoir (31, 8.35%). The predominant species was\n                      <jats:italic>Amblyomma variegatum<\/jats:italic>\n                      (85.44%) with\n                      <jats:italic>Rhipicephalus sanguineus<\/jats:italic>\n                      (s.l.) (0.27%) as the least occurring species. Total nucleic acid from the tick pools was screened for pathogens based on the nucleoprotein gene region in the S segment of the Crimean\u2013Congo haemorrhagic fever virus (CCHFV) genome, the 295\u2010bp fragment of the transposase gene of the\n                      <jats:italic>Coxiella burnetii<\/jats:italic>\n                      IS1111a element, the 560\u00a0bp segment of the ssrRNA gene of\n                      <jats:italic>Babesia<\/jats:italic>\n                      and\n                      <jats:italic>Theileria<\/jats:italic>\n                      , the 345\u00a0bp fragment of the\n                      <jats:italic>Ehrlichia<\/jats:italic>\n                      genus 16SrRNA gene and the rOmpA gene (OmpA) of\n                      <jats:italic>Rickettsia<\/jats:italic>\n                      . From the 52 tick pools screened, 40 (76.92%) were found positive for pathogen DNA. The pathogens identified were\n                      <jats:italic>Rickettsia africae<\/jats:italic>\n                      (69.23%),\n                      <jats:italic>Rickettsia aeschlimannii<\/jats:italic>\n                      (7.69%),\n                      <jats:italic>C. burnetii<\/jats:italic>\n                      (5.77%), uncultured\n                      <jats:italic>Ehrlichia<\/jats:italic>\n                      sp. (5.77%),\n                      <jats:italic>Candidatus<\/jats:italic>\n                      Midichloria mitochondrii (3.85%) and CCHFV (3.85%). A significant association was observed among\n                      <jats:italic>A. variegatum<\/jats:italic>\n                      ,\n                      <jats:italic>Hyalomma rufipes<\/jats:italic>\n                      ,\n                      <jats:italic>Hyalomma truncatum<\/jats:italic>\n                      and occurring tick\u2010borne pathogens\n                      <jats:italic>R. africae<\/jats:italic>\n                      ,\n                      <jats:italic>R. aeschlimannii<\/jats:italic>\n                      and uncultured\n                      <jats:italic>Ehrlichia<\/jats:italic>\n                      sp. (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The findings show the occurrence of zoonotic pathogens, suggesting an increased risk of infections among the abattoir workers. There is a need to adopt control measures within the abattoirs to prevent pathogen spread.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70030","type":"journal-article","created":{"date-parts":[[2024,9,17]],"date-time":"2024-09-17T14:28:13Z","timestamp":1726583293000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Ticks and tick\u2010borne pathogens in selected abattoirs and a slaughter slab in Kumasi, Ghana"],"prefix":"10.1002","volume":"10","author":[{"given":"Stacy","family":"Amoah","sequence":"first","affiliation":[{"name":"Department of Theoretical and Applied Biology College of Science KNUST  Kumasi Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy Martekai","family":"Unicorn","sequence":"additional","affiliation":[{"name":"Department of Theoretical and Applied Biology College of Science KNUST  Kumasi Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuella Tiwaa","family":"Kyeremateng","sequence":"additional","affiliation":[{"name":"Department of Theoretical and Applied Biology College of Science KNUST  Kumasi Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Genevieve","family":"Desewu","sequence":"additional","affiliation":[{"name":"Department of Theoretical and Applied Biology College of Science KNUST  Kumasi Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick Kwasi","family":"Obuam","sequence":"additional","affiliation":[{"name":"School of Public Health Kwame Nkrumah University of Science and Technology  Kumasi Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard Odoi\u2010Teye","family":"Malm","sequence":"additional","affiliation":[{"name":"Parasitology Department Noguchi Memorial Institute for Medical Research University of Ghana  Accra Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Osei\u2010Frempong","sequence":"additional","affiliation":[{"name":"Parasitology Department Noguchi Memorial Institute for Medical Research University of Ghana  Accra Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisca Adai","family":"Torto","sequence":"additional","affiliation":[{"name":"Parasitology Department Noguchi Memorial Institute for Medical Research University of Ghana  Accra Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen Kwabena","family":"Accorlor","sequence":"additional","affiliation":[{"name":"Parasitology Department Noguchi Memorial Institute for Medical Research University of Ghana  Accra Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5014-6153","authenticated-orcid":false,"given":"Kwadwo","family":"Boampong","sequence":"additional","affiliation":[{"name":"Department of Theoretical and Applied Biology College of Science KNUST  Kumasi Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5086-9714","authenticated-orcid":false,"given":"Sandra Abankwa","family":"Kwarteng","sequence":"additional","affiliation":[{"name":"Department of Theoretical and Applied Biology College of Science KNUST  Kumasi Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4470-4504","authenticated-orcid":false,"given":"Seth Offei","family":"Addo","sequence":"additional","affiliation":[{"name":"Department of Theoretical and Applied Biology College of Science KNUST  Kumasi Ghana"},{"name":"Parasitology Department Noguchi Memorial Institute for Medical Research University of Ghana  Accra Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John Asiedu","family":"Larbi","sequence":"additional","affiliation":[{"name":"Department of Theoretical and Applied Biology College of Science KNUST  Kumasi Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,9,17]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/S10493\u2010023\u201000808\u20100"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s44149\u2010022\u201000064\u20106"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00203\u2010023\u201003430\u20101"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2014.05.037"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879\u2010016\u20101660\u20106"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.07.016"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2011.0770"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmaid.2016.10.009"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2008.12.005"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/bf02310701"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2004.05.027"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168\u2010018\u20100524\u20102"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010650"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2007.12.030"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/jme\/tjv237"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10493\u2010012\u20109599\u20109"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2014.05.016"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.14202\/vetworld.2018.1357\u20101363"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2017.05.010"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.37.9.2745\u20102749.1999"},{"issue":"3","key":"e_1_2_13_22_1","first-page":"174","article-title":"Development of a duplex quantitative real\u2010time PCR assay for the detection of tick\u2010borne spotted fever group rickettsiae and Rickettsia rickettsii","volume":"78","author":"Jiang J.","year":"2005","journal-title":"Revue Internationale Des Services de Sant\u00e9 Des Forces Arm\u00e9es"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182004005967"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/mve.12094"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713\u201046\u20102\u2010611"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102180\u20106\u20102"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy096"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917\u2010023\u201003652\u2010x"},{"key":"e_1_2_13_29_1","doi-asserted-by":"publisher","DOI":"10.1101\/2020.11.10.376624"},{"key":"e_1_2_13_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.12.4.518"},{"key":"e_1_2_13_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000821"},{"key":"e_1_2_13_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2011.08.002"},{"key":"e_1_2_13_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PNTD.0000654"},{"key":"e_1_2_13_34_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.13\u20100251"},{"key":"e_1_2_13_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001982"},{"key":"e_1_2_13_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071\u2010022\u201005208\u20108"},{"key":"e_1_2_13_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.4.719\u2010756.2005"},{"key":"e_1_2_13_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00032\u201013"},{"issue":"2","key":"e_1_2_13_39_1","first-page":"177","article-title":"Molecular tools in the epidemiology of tick\u2010borne bacterial diseases","volume":"59","author":"Parola P.","year":"2001","journal-title":"Annals of Clinical Biology"},{"key":"e_1_2_13_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.06686\u201011"},{"key":"e_1_2_13_41_1","doi-asserted-by":"publisher","DOI":"10.14202\/vetworld.2019.1755\u20101759"},{"key":"e_1_2_13_42_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9091907"},{"key":"e_1_2_13_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469\u20100691.2008.02278.x"},{"key":"e_1_2_13_44_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006248"},{"key":"e_1_2_13_45_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20102915.2000.00246.x"},{"key":"e_1_2_13_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.13189"},{"issue":"2","key":"e_1_2_13_47_1","first-page":"132","article-title":"PCR\u2010based detection of Babesia spp. infection in collected ticks from cattle in West and North\u2010West of Iran","volume":"7","author":"Tavassoli M.","year":"2013","journal-title":"Journal of Arthropod\u2010Borne Diseases"},{"issue":"2","key":"e_1_2_13_48_1","first-page":"137","article-title":"Vectors of Crimean\u2010Congo hemorrhagic fever virus in Iran","volume":"9","author":"Telmadarraiy Z.","year":"2015","journal-title":"Journal of Arthropod\u2010Borne Diseases"},{"key":"e_1_2_13_49_1","volume-title":"Ticks of domestic animals in Africa: A guide to identification of species","author":"Walker A.","year":"2003"},{"key":"e_1_2_13_50_1","doi-asserted-by":"publisher","DOI":"10.1017\/s0007485399000619"},{"key":"e_1_2_13_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166\u20103542(04)00163\u20109"},{"key":"e_1_2_13_52_1","doi-asserted-by":"publisher","DOI":"10.1603\/0022\u20102585(2007)44"}],"container-title":["Veterinary Medicine and Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.70030","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/vms3.70030","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.70030","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T13:19:53Z","timestamp":1781875193000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/vms3.70030"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9]]},"references-count":51,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["10.1002\/vms3.70030"],"URL":"https:\/\/doi.org\/10.1002\/vms3.70030","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/VMS3.70030\/v3\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70030\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70030\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70030\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70030\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70030\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70030\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70030\/v3\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70030\/v2\/review1","asserted-by":"object"}]},"ISSN":["2053-1095","2053-1095"],"issn-type":[{"value":"2053-1095","type":"print"},{"value":"2053-1095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9]]},"assertion":[{"value":"2023-04-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70030"}}