{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T22:30:38Z","timestamp":1792103438056,"version":"4.4.0"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T00:00:00Z","timestamp":1727136000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100015690","name":"Universitas Padjadjaran","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100015690","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Brucellosis is an important global zoonosis caused by the bacterium\n                    <jats:italic>Brucella<\/jats:italic>\n                    sp. Brucellosis causes abortions, reproductive failure and reduced milk production, resulting in significant economic losses.\n                    <jats:italic>Brucella<\/jats:italic>\n                    species are reported to be resistant to antibiotics, which makes treatment difficult. The urgency of discovering new drug candidates to combat\n                    <jats:italic>Brucella<\/jats:italic>\n                    \u2019s infection necessitates the exploration of novel alternative agents with unique protein targets. Aminoacyl\u2010tRNA synthetases (aaRSs), which have fundamental functions in translation, inhibit this process, stop protein synthesis and ultimately inhibit bacterial growth. The purpose of this study was to isolate piperolactam A compounds from the methanol extract of\n                    <jats:italic>Piper betle<\/jats:italic>\n                    leaves that have potential as antibacterials to inhibit the growth of\n                    <jats:italic>Brucella<\/jats:italic>\n                    sp. causing brucellosis in livestock and to analyse the mechanism of inhibitory activity of piperolactam A compounds against the aaRS enzyme through a molecular docking approach in silico. Piperolactam A was isolated from\n                    <jats:italic>P. betle<\/jats:italic>\n                    by column chromatography and characterized by UV, IR, 1D and 2D NMRs and MS, then tested for their inhibition mechanism against the enzymes threonyl\u2010tRNA synthetase, leucyl\u2010tRNA synthetase (LeuRS) and methionyl\u2010tRNA synthetase in silico. The result in silico test is that piperolactam A has the potential to inhibit LeuRS enzyme with the greater binding affinity.\n                  <\/jats:p>","DOI":"10.1002\/vms3.70042","type":"journal-article","created":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T09:07:51Z","timestamp":1727168871000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Potential Piperolactam A Isolated From\n                    <i>Piper betle<\/i>\n                    as Natural Inhibitors of\n                    <i>Brucella<\/i>\n                    Species Aminoacyl\u2010tRNA Synthetase for Livestock Infections: In Silico Approach"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1295-2765","authenticated-orcid":false,"given":"Diding","family":"Latipudin","sequence":"first","affiliation":[{"name":"Department of Animal Nutrition Faculty of Animal Husbandry Universitas Padjadjaran Sumedang West Java Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5675-8863","authenticated-orcid":false,"given":"Sefren Geiner","family":"Tumilaar","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Mathematics and Natural Sciences Universitas Padjadjaran Sumedang West Java Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoga","family":"Ramdani","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Mathematics and Natural Sciences Universitas Padjadjaran Sumedang West Java Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2337-5289","authenticated-orcid":false,"given":"Dudi","family":"Dudi","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition Faculty of Animal Husbandry Universitas Padjadjaran Sumedang West Java Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9789-9785","authenticated-orcid":false,"given":"Dikdik","family":"Kurnia","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Mathematics and Natural Sciences Universitas Padjadjaran Sumedang West Java Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,9,24]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.compbiolchem.2017.01.004"},{"key":"e_1_2_10_3_1","first-page":"1","article-title":"Two New Anticancer Phenolic Derivatives From Leaves of Piper betle Linn","volume":"35","author":"Atiya A.","year":"2020","journal-title":"Natural Product 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