{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T00:32:38Z","timestamp":1792542758889,"version":"4.5.0"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,9,27]],"date-time":"2024-09-27T00:00:00Z","timestamp":1727395200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Blastocystis<\/jats:italic> spp. is one of the most common protozoa worldwide, living in the gastrointestinal tract of humans and many other animals. On the basis of the genetic heterogeneity of small subunit ribosomal RNA, at least 28 subtypes (ST1\u2010ST17, ST21 and ST23\u2013ST32) are reported to exist in mammals and birds.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>This study was carried out to determine the prevalence and subtypes of <jats:italic>Blastocystis<\/jats:italic> spp. in Anatolian buffaloes (<jats:italic>Bubalus bubalis<\/jats:italic>) in Van province in the Eastern Anatolia Region of Turkey.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>DNA was extracted using commercial GeneMATRIX Stool DNA Purification Kit and then stored at \u221220\u00b0C until PCR amplification. After PCR amplification of the SSU rRNA gene region positive <jats:italic>Blastocystis<\/jats:italic> spp., amplicons from buffalo faeces were sequenced and then deposited in GenBank (OR576949.1, OR576950.1, OR576970.1, OR576971.1, OR577019.1, PP837943.1, PP837940.1, PP837939.1, PP837604.1, PP837937.1, PP837934.1, PP837601.1, PP837936.1 and PP837603.1).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>PCR analysis of 120 faecal samples showed a total prevalence of 11.67% (14\/120). The prevalence was higher in females and young animals (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a00.05). Sequence analysis revealed <jats:italic>Blastocystis<\/jats:italic> spp., ST10, ST14, ST25 and ST26 subtypes. To our knowledge, <jats:italic>Blastocystis<\/jats:italic> subtypes ST25 and ST26 in buffaloes were reported for the first time in this study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>It is thought that more large\u2010scale studies should be carried out to determine the zoonotic subtype potential of this protozoan in the region.<\/jats:p><\/jats:sec>","DOI":"10.1002\/vms3.70054","type":"journal-article","created":{"date-parts":[[2024,9,27]],"date-time":"2024-09-27T10:13:45Z","timestamp":1727432025000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Prevalence and Subtypes Distribution (ST10, ST14, ST25, ST26) of <i>Blastocystis<\/i> spp. in Anatolian Water Buffalo (<i>Bubalus bubalis<\/i>) in Van, T\u00fcrkiye"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6564-3416","authenticated-orcid":false,"given":"Adnan","family":"Ayan","sequence":"first","affiliation":[{"name":"Department of Genetics Faculty of Veterinary Medicine Van Yuzuncu Yil University Van Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0130-970X","authenticated-orcid":false,"given":"Burcak","family":"Aslan Celik","sequence":"additional","affiliation":[{"name":"Department of Parasitology Faculty of Veterinary Medicine Siirt University Siirt Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6365-2688","authenticated-orcid":false,"given":"Ozgur Yasar","family":"Celik","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Faculty of Veterinary Medicine Siirt University Siirt Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0749-2418","authenticated-orcid":false,"given":"Ali Bilgin","family":"Yilmaz","sequence":"additional","affiliation":[{"name":"School of Health Van Yuzuncu Yil University Van Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6233-7109","authenticated-orcid":false,"given":"Ozlem","family":"Orunc Kilinc","sequence":"additional","affiliation":[{"name":"\u00d6zalp Vocational School Van Yuzuncu Yil University Van Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2577-3774","authenticated-orcid":false,"given":"Ozge","family":"Oktay Ayan","sequence":"additional","affiliation":[{"name":"Department of Parasitology Faculty of Medicine Van Yuzuncu Yil University Van Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,9,27]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.protis.2019.125679"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.24425\/pjvs.2023.145022"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436\u2010019\u201006243\u20108"},{"issue":"1","key":"e_1_2_10_5_1","first-page":"6","article-title":"Subtype Analyses of 50 Blastocystis spp. Isolates From Symptomatic Patients Distirbution Determination","volume":"3","author":"B\u00fcy\u00fckbaba B. \u00d6.","year":"2017","journal-title":"Journal of Health Sciences of Kocaeli University"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.5455\/ajvs.64195"},{"key":"e_1_2_10_7_1","first-page":"59","article-title":"Blastocystis sp: A Neglected Zoonotic Protozoan","volume":"3","author":"Daryani A.","year":"2008","journal-title":"Proceedings of the ASEAN Congress of Tropical Medicine and Parasitology"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304\u20104017(97)00224\u20100"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.5578\/mb.8532"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9081656"},{"key":"e_1_2_10_11_1","unstructured":"Hall T. 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