{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:16:00Z","timestamp":1772118960101,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T00:00:00Z","timestamp":1728950400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The high prevalence of leptospirosis in humans is of great public health concern, particularly in tropical and subtropical regions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study aimed to determine the seroprevalence of leptospiral antibodies and the distribution of serovars in livestock breeders and farmers in Golestan province.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Seventy samples of serum collected from farmers and ranchers suspected of leptospirosis were examined using an ELISA method for surveying Immunoglobulin M (IgM) anti\u2010leptospiria. Also from samples, DNA was extracted and PCR was performed using by primers for\n                      <jats:italic>16s rRNA<\/jats:italic>\n                      . Demographic properties of positive patients were analysed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Chi\u2010square statistical test shows a statistically significant difference between the gender and prevalence leptospirosis (\n                      <jats:italic>p<\/jats:italic>\n                      \u2010value = 0.004). Also, by examining the age, it was shown that 68.57% of patients are in the middleaged rang. According to the results obtained from the study and investigation of blood serum IgM\u2010and Immunoglobulin G (IgG) in people suspected of leptospirosis, 3 cases of the patients had IgM higher than 11 and were known to be positive for leptospirosis. Also, by examining the IgG level of patients, 5 cases had intermediate results, and 2 cases were found to be positive for IgG. The PCR results showed that 41.42% of patients tested positive for the\n                      <jats:italic>16s rRNA<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Leptospirosis is a common disease among farmers, and in Golestan province, considering traditional farming methods, it is considered an important infectious disease. Therefore, health and safety measures should be expanded to control and prevent this disease. Also, by employing mechanised agricultural methods, the prevalence of leptospirosis in this region can be significantly reduced.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70065","type":"journal-article","created":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T03:34:14Z","timestamp":1728963254000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Study on Seroprevalence and Leptospiral Antibody Distribution Among Livestock Breeders and Farmers in Golestan Province"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3723-4340","authenticated-orcid":false,"given":"Malihe","family":"Naderi","sequence":"first","affiliation":[{"name":"Hiroshima Institute of Life Sciences, 7\u201021, Nishi Asahi\u2010Machi  Hiroshima\u2010shi Hiroshima Japan"},{"name":"Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology Shahid Behehsti University  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vahideh Hamidi","family":"Sofiani","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine Golestan University of Medical Science  Gorgan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reza","family":"Hoseinpour","sequence":"additional","affiliation":[{"name":"Epidemiology, Health Management and Social Development Research Center Golestan University of Medical Sciences  Gorgan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amir Mohammad","family":"Alborzi","sequence":"additional","affiliation":[{"name":"Neuroscience Research Center Golestan University of Medical Sciences  Gorgan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4831-766X","authenticated-orcid":false,"given":"Seyed Amir","family":"Soltani","sequence":"additional","affiliation":[{"name":"Hospital Administration Research center, Sari Branch Islamic Azad University  Sari Iran"},{"name":"Social Determinants of Health Research Center Health Research Institute Golestan University of Medical Sciences  Gorgan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,10,15]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.15789\/2220\u20107619\u2010MAG\u201017028"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.5455\/msm.2020.32.15\u201019"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102334\u201010\u201091"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani11010191"},{"issue":"1","key":"e_1_2_11_6_1","first-page":"15","article-title":"Interpretation of Microscopic Agglutination Test for Leptospirosis Diagnosis And Seroprevalence","volume":"4","author":"Chintana C.","year":"2014","journal-title":"Asian Pacific Journal of Tropical Biomedicine"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/oemed\u20102016\u2010103895"},{"key":"e_1_2_11_8_1","first-page":"110","article-title":"Seroinvestigation of Bovine Leptospirosis in Shahrekord District, Central Iran","volume":"5","author":"Ebrahimi A.","year":"2004","journal-title":"Iranian Journal of Veterinary Research"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-45059-8_6"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20103\u2010662\u201045059\u20108_5"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/tropicalmed7100260"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03474.x"},{"key":"e_1_2_11_13_1","volume-title":"Instruction Booklet of International Course on Laboratory Methods for the Diagnosis of Leptospirosis","author":"Hartskeel R. A.","year":"2001"},{"issue":"4","key":"e_1_2_11_14_1","first-page":"353","article-title":"Evaluation of Patients Sera for Early Diagnosis of Leptospirosis by PCR","volume":"9","author":"Honarmand H.","year":"2009","journal-title":"Journal of Ardabil University of Medical Sciences"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40001\u2010022\u201000902\u2010w"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph18179411"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrp.2016.04.006"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/7286918"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.19\u20100003"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/tmi.13033"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0008970"},{"key":"e_1_2_11_22_1","volume-title":"Leptospirosis: Laboratory Manual","author":"World Health Organization","year":"2007"}],"container-title":["Veterinary Medicine and Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.70065","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T10:02:32Z","timestamp":1745488952000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/vms3.70065"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,15]]},"references-count":21,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,11]]}},"alternative-id":["10.1002\/vms3.70065"],"URL":"https:\/\/doi.org\/10.1002\/vms3.70065","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/VMS3.70065\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70065\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70065\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70065\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70065\/v1\/review2","asserted-by":"object"}]},"ISSN":["2053-1095","2053-1095"],"issn-type":[{"value":"2053-1095","type":"print"},{"value":"2053-1095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,15]]},"assertion":[{"value":"2024-05-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70065"}}