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The synthesized carbon dots underwent comprehensive characterization through a range of spectroscopic and microscopic techniques. These included UV\u2010Vis, FTIR, fluorescence spectroscopy and HR\u2010TEM, DLS instruments. HR\u2010TEM results exhibited the presence of homogenous spherical\u2010shaped C\u2010dots of about 3.3\u00a0nm without aggregates. Furthermore, the prepared CCDs were studied for their in vivo analgesic effect in mice by performing tail\u2010immersion, hot plate and acetic acid writhing tests. Also, an MTT assay was performed to assess the in vitro cytotoxicity of CCDs against L929 cells. In vitro cytotoxicity studies revealed that L929 cells exhibited higher cell viability when treated with prepared CCDs. The cellular uptake studies revealed the phase contrast images of MG\u201063 cells at wavelength 488\u00a0nm clearly depicted the aggregation of green, fluorescent CCDs within the cells while leaving nuclei unobscured. In addition, to the best of our understanding, the results presented in this paper showed that CCDs exhibited an important analgesic effect and enhanced anti\u2010nociceptive activity, which may be due to stimulation of the opioidergic system. Consequently, CCDs appear to be a viable analgesic alternative for traditional analgesic candidates in pain management.<\/jats:p>","DOI":"10.1002\/vms3.70090","type":"journal-article","created":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T13:23:47Z","timestamp":1730726627000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A Novel <i>Cortex Phellodendri Chinensis<\/i>\u2013Based Carbon Dots Platform for Remarkable Analgesia for Clinical Pain Management"],"prefix":"10.1002","volume":"10","author":[{"given":"Huimin","family":"Peng","sequence":"first","affiliation":[{"name":"Department of Pain Xiangyang Central Hospital Affiliated Hospital of Hubei University of Arts and Science  Xiangyan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingxing","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Pain Xiangyang Central Hospital Affiliated Hospital of Hubei 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