{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T02:00:08Z","timestamp":1792634408365,"version":"4.5.0"},"reference-count":86,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T00:00:00Z","timestamp":1730678400000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      This study looked at how different concentrations of curcumin (Curc), zinc chloride (ZnCl\n                      <jats:sub>2<\/jats:sub>\n                      ), zinc oxide nanoparticles (ZnO\u2010NPs) and Curc loaded on ZnO\u2010NPs (Curc\u2010co\u2010ZnO\u2010NPs) in cryopreservation dilution affected the quality of ram sperm after thawing.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      ZnO\u2010NPs were synthesised using\n                      <jats:italic>Berberis vulgaris<\/jats:italic>\n                      leaf aqueous extract. Then, Curc was loaded on the ZnO\u2010NPs that had been synthesised. We used analytical methods to look at the composition, morphology and size of green synthesised ZnO\u2010NPs and Curc\u2010co\u2010ZnO\u2010NPs, including UV\u2010Vis, zeta potential, EDX, DLS, FE\u2010SEM and FT\u2010IR. Using a Tris\u2010base extender containing various concentrations of Curc, ZnCl\n                      <jats:sub>2<\/jats:sub>\n                      , ZnO\u2010NPs and Curc\u2010co\u2010ZnO\u2010NPs (0, 1, 10 and 100\u00a0\u00b5g\/mL), semen samples from four rams were combined. Sperm motility, viability, DNA and plasma membrane integrity, total abnormalities and malondialdehyde (MDA) generation were all evaluated in treatment groups after thawing.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The results showed that adding 1\u00a0\u00b5g\/mL of ZnO\u2010NPs and Curc\u2010co\u2010ZnO\u2010NPs significantly reduced the level of MDA and total abnormalities (\n                      <jats:italic>p\u00a0<\/jats:italic>\n                      &lt;\u00a00.05). Additionally, following the freeze\u2010thawing procedure, the presence of 1\u00a0\u00b5g\/mL of Curc\u2010co\u2010ZnO\u2010NPs in the diluent of ram sperm significantly increased the percentage of sperm viability and motility in comparison to the control and other treatment groups (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). Furthermore, as compared to the control group and other treatments, treatments containing 1\u00a0\u00b5g\/mL of Curc\u2010co\u2010ZnO\u2010NPs significantly improved membrane and DNA integrity (\n                      <jats:italic>p\u00a0<\/jats:italic>\n                      &lt;\u00a00.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>It appears that following freeze\u2010thawing, the Curc\u2010co\u2010ZnO\u2010NPs (1\u00a0\u00b5g\/mL) enhanced sperm parameters.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70091","type":"journal-article","created":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T08:43:56Z","timestamp":1730709836000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Comparison of the Effect of Adding Different Levels of Zinc Chloride, Curcumin, Zinc Oxide Nanoparticles (Zano\u2010NPs), Curcumin Loaded on Zano\u2010NPs on Post\u2010Thawing Quality of Ram Semen"],"prefix":"10.1002","volume":"10","author":[{"given":"Fatemeh","family":"Omidi","sequence":"first","affiliation":[{"name":"Department of Animal Science Faculty of Agricultural and Engineering Science Razi University  Kermanshah Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hadi","family":"Hajarian","sequence":"additional","affiliation":[{"name":"Department of Animal Science Faculty of Agricultural and Engineering Science Razi University  Kermanshah Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4810-2415","authenticated-orcid":false,"given":"Hamed","family":"Karamishabankareh","sequence":"additional","affiliation":[{"name":"Department of Animal Science Faculty of Agricultural and Engineering Science Razi University  Kermanshah Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leila","family":"Soltani","sequence":"additional","affiliation":[{"name":"Department of Animal Science Faculty of Agricultural and Engineering Science Razi University  Kermanshah Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mojtaba","family":"Dashtizad","sequence":"additional","affiliation":[{"name":"Department of Animal Science National Institute of Genetics and Biotechnology  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,4]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/153573"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439\u20100531.2012.02049.x"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2008.05.003"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13253"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.4103\/jhrs.JHRS_150_18"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2015.08.008"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.5998"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13523"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0737\u20100806(87)80025\u20104"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10561-016-9566-5"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1166\/jnn.2005.184"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1515\/gps\u20102017\u20100014"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.22034\/nmrj.2021.02.005"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.52547\/sjrm.5.1.10"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.20546\/ijcmas.2016.512.090"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2005.06.025"},{"issue":"9","key":"e_1_2_11_18_1","first-page":"767","article-title":"Effect of Zinc Oxide Nanoparticles on Viability of Human Spermatozoa","volume":"11","author":"Barkhordari A.","year":"2013","journal-title":"Iranian Journal of Reproductive Medicine"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0026012"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2018.11.101"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439\u20100272.2010.01146.x"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2010.09.001"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0015\u20100282(97)00241\u20100"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/asj.12395"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439\u20100272.2004.00626.x"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nutres.2008.11.007"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.4239\/wjd.v6.i2.333"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476\u20104431.2009.00418.x"},{"issue":"2","key":"e_1_2_11_29_1","first-page":"149","article-title":"Protective Effects of Nano\u2010ZnO on the Primary Culture Mice Intestinal Epithelial Cells in In Vitro Against Oxidative Injury","volume":"6","author":"Dawei A. I.","year":"2010","journal-title":"World Journal of Agricultural Sciences"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2006.01.047"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1080\/2090598X.2019.1599624"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2016.01.014"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.106.056705"},{"key":"e_1_2_11_34_1","volume-title":"Salamons' Artificial Insemination of Sheep and Goats","author":"Evans G.","year":"1987"},{"issue":"1","key":"e_1_2_11_35_1","first-page":"49","article-title":"Zinc Oxide Nanoparticles Have Beneficial Effect on Frozen\u2010Thawed Spermatozoa of Holstein Bulls","volume":"12","author":"Farhadi F.","year":"2022","journal-title":"Iranian Journal of Applied Animal Science"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcam.2022.100736"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2017.10.001"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8TB01796D"},{"issue":"2","key":"e_1_2_11_39_1","first-page":"120","article-title":"Protective Effect of Curcumin Against Gamma\u2010Radiation on Testis of Rats","volume":"18","author":"Hamzavi J. Z.","year":"2014","journal-title":"Hormozgan Medical Journal"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2018.05.012"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2017.10.112"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2011.12.002"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.109278"},{"key":"e_1_2_11_44_1","first-page":"295","article-title":"The Effect of Curcumin on Frozen\u2010Thawed Sperm Quality and Fertility of Broiler Breeder Roosters","volume":"50","author":"Jalili F.","year":"2020","journal-title":"Iranian Journal of Animal Science"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2023.107012"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2022.02.015"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815\u2010012\u20109894\u20108"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1089\/adt.2007.064"},{"issue":"3","key":"e_1_2_11_49_1","first-page":"38","article-title":"Sperm Nuclear Zinc, Chromatin Stability, and Male Fertility","volume":"1","author":"Kvist U.","year":"1987","journal-title":"Scanning Microscopy"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2014.09.005"},{"issue":"4","key":"e_1_2_11_51_1","first-page":"241","article-title":"Associations Between Sperm Abnormalities, Breed, Age, and Scrotal Circumference in Beef Bulls","volume":"75","author":"Menon A. G.","year":"2011","journal-title":"Canadian Journal of Veterinary Research"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2014.10.009"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2014.09.005"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/vms3.580"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1177\/0040517520920952"},{"issue":"4","key":"e_1_2_11_56_1","first-page":"964","article-title":"Assessment of Optimal Osmotic Pressure of Citrate Egg Yolk Extender for Cryopreservation of Buffalo Bull (Bubalus bubalis) Semen","volume":"23","author":"Mughal D. H.","year":"2013","journal-title":"Journal of Animal & Plant Sciences"},{"issue":"2","key":"e_1_2_11_57_1","first-page":"62","article-title":"The Effect of Curcumin on Intracellular pH (pHi), Membrane Hyperpolarization and Sperm Motility","volume":"15","author":"Naz R. K.","year":"2014","journal-title":"Journal of Reproduction & Infertility"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477\u20107827\u20102\u201075"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20040965"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00580-017-2452-0"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.28.1_supplement.759.1"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.17221\/8677\u2010VETMED"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2012.08.568"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.1039\/d0ra05755j"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.1080\/10667857.2020.1863557"},{"key":"e_1_2_11_66_1","doi-asserted-by":"publisher","DOI":"10.1080\/07315724.2009.10719780"},{"key":"e_1_2_11_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378\u20104320(94)90055\u20108"},{"key":"e_1_2_11_68_1","doi-asserted-by":"publisher","DOI":"10.21608\/jappmu.2016.48748"},{"key":"e_1_2_11_69_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-50221-1"},{"key":"e_1_2_11_70_1","doi-asserted-by":"publisher","DOI":"10.1007\/s43032\u2010021\u201000572\u20109"},{"key":"e_1_2_11_71_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac200853a"},{"key":"e_1_2_11_72_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010017\u20100004\u20103"},{"key":"e_1_2_11_73_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10616\u2010016\u20100027\u20106"},{"key":"e_1_2_11_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jece.2021.105215"},{"key":"e_1_2_11_75_1","doi-asserted-by":"publisher","DOI":"10.1111\/php.12281"},{"issue":"3","key":"e_1_2_11_76_1","first-page":"185","article-title":"Effect of Curcumin on Rat Sperm Morphology After the Freeze\u2010Thawing process","volume":"4","author":"Soleimanzadeh A.","year":"2013","journal-title":"Veterinary Research Forum"},{"key":"e_1_2_11_77_1","doi-asserted-by":"publisher","DOI":"10.54680\/fr23610110712"},{"key":"e_1_2_11_78_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.14130"},{"key":"e_1_2_11_79_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003\u20109861(92)90040\u20104"},{"key":"e_1_2_11_80_1","doi-asserted-by":"publisher","DOI":"10.15414\/jmbfs.2015.4.special2.120\u2010124"},{"key":"e_1_2_11_81_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9RA00536F"},{"key":"e_1_2_11_82_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166\u20101280(02)00242\u20107"},{"key":"e_1_2_11_83_1","doi-asserted-by":"publisher","DOI":"10.2174\/138161213805289219"},{"key":"e_1_2_11_84_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2017.08.021"},{"key":"e_1_2_11_85_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S208332"},{"key":"e_1_2_11_86_1","doi-asserted-by":"publisher","DOI":"10.1080\/00071668.2019.1686125"},{"key":"e_1_2_11_87_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.13491"}],"container-title":["Veterinary Medicine and Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.70091","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T10:03:58Z","timestamp":1745489038000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/vms3.70091"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11]]},"references-count":86,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,11]]}},"alternative-id":["10.1002\/vms3.70091"],"URL":"https:\/\/doi.org\/10.1002\/vms3.70091","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/VMS3.70091\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70091\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70091\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70091\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70091\/v3\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70091\/v3\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70091\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70091\/v3\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70091\/v2\/review1","asserted-by":"object"}]},"ISSN":["2053-1095","2053-1095"],"issn-type":[{"value":"2053-1095","type":"print"},{"value":"2053-1095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11]]},"assertion":[{"value":"2024-03-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70091"}}