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For this purpose, sperm samples were collected from 12 2\u2010year\u2010old male Gloster canaries three times a week using cloacal massage for 4 weeks. After individual evaluation, sperm samples from the canaries were combined. Mixed sperm were divided into two groups in the study. Overall, 8% dimethyl sulfoxide (DMSO) and ethylene glycol (EG) were used as cryoprotectants. Sperm samples were drawn into straws after adding Dulbecco's Modified Eagle Medium (DMEM) extender with high glucose ratio and two different cryoprotectants in a 1:1 ratio and frozen to \u221280\u00b0C with liquid nitrogen vapour and then stored in liquid nitrogen at \u2212196\u00b0C. Frozen\u2010thawed semen samples were evaluated regarding motility, vitality, plasma membrane integrity (hypoosmotic swelling test [HOST]), density and abnormal spermatozoa rate. The highest motility value after freezing and thawing was determined in the EG group with 31.667%\u00a0\u00b1\u00a04.773%. In addition, vitality, plasma membrane integrity and normal sperm morphology were statistically significantly higher in the EG\u2010frozen group, whereas head and tail abnormality was low (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). This study determined that a DMEM extender containing 8% EG was more advantageous than a DMEM containing DMSO regarding spermatological parameters and could be used for long\u2010term storage of canary sperm.<\/jats:p>","DOI":"10.1002\/vms3.70101","type":"journal-article","created":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T09:47:15Z","timestamp":1730281635000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Sperm Cryopreservation in Canaries to Protect Endangered Songbird Species: Comparison of Different Cryoprotectants"],"prefix":"10.1002","volume":"10","author":[{"given":"Arda","family":"Onur \u00d6zk\u00f6k","sequence":"first","affiliation":[{"name":"Department of Veterinary Amasya University Suluova Vocational School  Amasya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5728-1478","authenticated-orcid":false,"given":"Burcu","family":"Esin","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination Faculty of Veterinary Medicine University of Ondokuz Mayis  Samsun Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eser","family":"Akal","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination Faculty of Veterinary Medicine University of Ondokuz Mayis  Samsun Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,30]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u2010981\u201010\u20103975\u20101_4"},{"key":"e_1_2_10_3_1","volume-title":"Techniques for Semen Evaluation, Semen Storage, and Fertility Determination","author":"Bakst M. 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