{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T17:51:01Z","timestamp":1792259461408,"version":"4.4.0"},"reference-count":80,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T00:00:00Z","timestamp":1736467200000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Ram sperm are more vulnerable to freezing than those of most other farm animals. During sperm freezing, the cell membrane loses some of its cholesterol, which regulates signalling mechanisms and prevents premature capacitation. Resveratrol (RES) increases the fluidity of the cell membrane, which becomes peroxidized during freezing and reduces free radicals. In this study, the effectiveness of RES, cholesterol\u2010loaded cyclodextrin (CLC) and their combinations in ram sperm cryopreservation were investigated. The collected semen was divided into two equal volumes: One was diluted with tris\u2010citric acid\u2010glucose medium (TCG) containing CLC, whereas the other was diluted with a CLC\u2010free TCG solution. After examining motility, both groups were further divided into two equal volumes, forming the following working groups: control (no RES, no CLC); RES (20\u00a0\u00b5g\/mL); CLC (2\u00a0mg CLC\/120\u00a0\u00d7\u00a010\n                    <jats:sup>6<\/jats:sup>\n                    sperm); and RES\u00a0+\u00a0CLC (RES 20\u00a0\u00b5g\/mL\u00a0+\u00a02\u00a0mg CLC\/120\u00a0\u00d7\u00a010\n                    <jats:sup>6<\/jats:sup>\n                    sperm). These groups were diluted with media containing their respective additives. Post\u2010thawing, the samples were analysed for motility, acrosome and membrane integrity, membrane functionality, mitochondrial activity, capacitation status, oxidative stress and DNA integrity. CLC preserved sperm total motility, acrosome and plasma membrane integrity and decreased the rate of early capacitation (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.05). RES had no significant effect on sperm quality before freezing and post\u2010thawing (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&gt;\u00a00.05). However, RES\u00a0+\u00a0CLC increased mitochondrial activity post\u2010thawing (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.05). In conclusion, CLC minimized sperm membrane damage caused by cryopreservation in ram sperm. RES alone was ineffective, and the combination of RES and CLC did not yield a positive synergistic effect on ram spermatological parameters.\n                  <\/jats:p>","DOI":"10.1002\/vms3.70172","type":"journal-article","created":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T10:30:18Z","timestamp":1736505018000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The Effect of Cholesterol\u2010Loaded Cyclodextrin and Resveratrol Compounds on Post\u2010Thawing Quality of Ram Semen"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1326-2678","authenticated-orcid":false,"given":"Eser","family":"Ahmet","sequence":"first","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine Siirt University  Siirt Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ar\u0131c\u0131","family":"Ramazan","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine \u0130stanbul University\u2014Cerrahpasa  Avcilar \u0130stanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya\u011fc\u0131o\u011flu","family":"Selin","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine \u0130stanbul University\u2014Cerrahpasa  Avcilar \u0130stanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandal Asiye","family":"\u0130zem","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine \u0130stanbul University\u2014Cerrahpasa  Avcilar \u0130stanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ersoy","family":"Nur","sequence":"additional","affiliation":[{"name":"Graduate Education Institute Istanbul University\u2014Cerrahpasa  Avc\u0131lar \u0130stanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Demir","family":"Kamber","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine \u0130stanbul University\u2014Cerrahpasa  Avcilar \u0130stanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evecen","family":"Mithat","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine \u0130stanbul University\u2014Cerrahpasa  Avcilar \u0130stanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ak","family":"Kemal","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine \u0130stanbul University\u2014Cerrahpasa  Avcilar \u0130stanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2020.04.034"},{"issue":"2","key":"e_1_2_9_3_1","first-page":"33","article-title":"Effects of Extender Osmolarity, Cooling Rate, Dilution Rate and Glycerol Addition Time on Post\u2010Thaw Ram Semen Characteristics and Fertilization","volume":"36","author":"Ak K.","year":"2010","journal-title":"\u0130stanbul \u00dcniversitesi Veteriner Fak\u00fcltesi Dergisi"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jksus.2020.10.023"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2021.106697"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2007.01.014"},{"issue":"5","key":"e_1_2_9_7_1","first-page":"336","article-title":"Pre\u2010Freezing Treatment With Butylated Hydroxytoluene and Cholesterol\u2010Loaded Methyl\u2010\u03b2\u2010Cyclodextrin Improves Quality of Cryopreserved Boar Semen","volume":"39","author":"Baishya S. 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