{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T01:31:16Z","timestamp":1785461476250,"version":"3.56.0"},"reference-count":64,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,2,28]],"date-time":"2025-02-28T00:00:00Z","timestamp":1740700800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study was conducted to examine the effect of dietary natural compounds, such as \u03b2\u2010glucan, carotenoids, oligosaccharides and anthocyanins, on immune response and tight junction proteins in broiler chickens. A total of 900 one\u2010day\u2010old chickens were allocated to five treatments in three floor pens (replicates) of 60 broilers per pen. Chickens were fed five diets: a control (basal) diet, a diet supplemented with \u03b2\u2010glucan at 0.05%, or diets supplemented with carotenoids, oligosaccharides or anthocyanins at 0.5% of each compound. Male broilers were randomly selected for sample collections. On Day 25, plasma samples were collected from the brachial vein. On Day 26, six broilers were intraperitoneally injected with 2\u00a0mg of lipopolysaccharide per kg of body weight. Twelve hours later (Day 27), blood and ileum samples were collected to determine immune parameters and tight junction proteins using ELISA assays. The results showed that anthocyanin supplementation reduced the level of interleukin\u20101\u03b2 compared to the lipopolysaccharide\u2010injected control group (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0=\u00a00.047), which suggests that anthocyanin could partly alleviate the inflammation. Carotenoids reached a lower level of interleukin\u20106 compared to the \u03b2\u2010glucan treatment (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0=\u00a00.0466). \u03b2\u2010Glucan (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0=\u00a00.0382) and oligosaccharides (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0=\u00a00.0449) increased the level of plasma immunoglobulin G compared to the challenged control group, which may indicate an enhanced humoral immunity. Furthermore, \u03b2\u2010glucan (except for occludin 2), carotenoids, oligosaccharides and anthocyanins increased (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.05) the levels of ileal zonula occludens\u20101, occludin 1 and occludin 2 compared to the lipopolysaccharide\u2010challenged control chickens. This may suggest that all the bioactive substances improved the gut barrier function. The plasma levels of tight junction proteins show higher concentrations in lipopolysaccharide\u2010challenged groups compared to the non\u2010challenged groups (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.05). This may refer to the tight junction disruption and appearance in circulation as a reflection of lipopolysaccharide exposure.\n                  <\/jats:p>","DOI":"10.1002\/vms3.70175","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T05:09:54Z","timestamp":1741756194000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Impact of Selected Natural Bioactive Substances on Immune Response and Tight Junction Proteins in Broiler Chickens"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-0713-5882","authenticated-orcid":false,"given":"Brigitta","family":"Csernus","sequence":"first","affiliation":[{"name":"Department of Evolutionary Zoology and Human Biology University of Debrecen  Debrecen Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgina","family":"Pesti\u2010Asb\u00f3th","sequence":"additional","affiliation":[{"name":"Doctoral School of Animal Science University of Debrecen  Debrecen Hungary"},{"name":"Center for Complex Systems and Microbiome Innovations, Faculty of Agricultural and Food Sciences and Environmental Management University of Debrecen  Debrecen Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judit","family":"Remenyik","sequence":"additional","affiliation":[{"name":"Center for Complex Systems and Microbiome Innovations, Faculty of Agricultural and Food Sciences and Environmental Management University of Debrecen  Debrecen Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e1ndor","family":"Bir\u00f3","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, Faculty of Medicine University of Debrecen  Debrecen Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00e1szl\u00f3","family":"Babinszky","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition Physiology, Institute of Animal Science, Biotechnology and Nature Conservation, Faculty of Agricultural and Food Sciences and Environmental Management University of Debrecen  Debrecen Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00e1szl\u00f3","family":"St\u00fcndl","sequence":"additional","affiliation":[{"name":"Institute of Food Technology, Faculty of Agricultural and Food Sciences and Environmental Management University of Debrecen  Debrecen Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e1nos","family":"Ol\u00e1h","sequence":"additional","affiliation":[{"name":"Farm and Regional Research Institute of Debrecen University of Debrecen  Debrecen 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