{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T17:47:17Z","timestamp":1790876837629,"version":"4.1.0"},"reference-count":67,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T00:00:00Z","timestamp":1743206400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T00:00:00Z","timestamp":1743206400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The embryo co\u2010culture systems with the monolayer cultured cells are complex, unreproducible, and have a high probability of biological contamination. Therefore, nowadays, using conditioned media is a suitable alternative to these methods.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study aimed to investigate the impact of utilizing bovine oviductal epithelial cell\u2010derived conditioned medium (bOEC\u2010CM) on subsequent embryo development and quality.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Bovine embryos produced in vitro were cultured in a specific medium supplemented with either 5% charcoal\u2010stripped FBS (Charcoled Strip Serum [CSS]) or 10% bOEC\u2010CM from either Days 1 or 3 post\u2010fertilization. Various parameters, such as cleavage rate, blastocyst formation, hatching rate and blastocyst quality, were assessed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The results indicated that adding 10% CM from Day 1 significantly reduced the cleavage rate compared to using CSS on the same day (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). Furthermore, the CSS and CM from both Days 1 and 3 increased blastocyst formation rates (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). Notably, the addition of 10% CM on Day 3 significantly improved the hatching rate compared to the other groups (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). Both CM and CSS were found to enhance the inner cell mass (ICM), trophectoderm (TE) and total cell numbers in blastocysts when used on both Days 1 and 3 (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). Additionally, CM from Day 3 positively influenced the expression levels of development\u2010specific genes in cultured embryos (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Overall, the findings suggest that using bOEC\u2010CM at a 10% concentration may provide a promising supplement even better than serum and traditional co\u2010culture methods during the last 5 days of embryo culture.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70179","type":"journal-article","created":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T14:03:46Z","timestamp":1743429826000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Improving Bovine Embryo Development and Quality Using Bovine Oviductal Epithelial Cell\u2010Derived Conditioned Medium (bOEC\u2010CM)"],"prefix":"10.1002","volume":"11","author":[{"given":"Saeed Kazemi","family":"Hosseinabadi","sequence":"first","affiliation":[{"name":"Department of Animal Physiology Faculty of Basic Sciences Shahrekord University  Shahrekord Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3773-4847","authenticated-orcid":false,"given":"Hassan","family":"Nazari","sequence":"additional","affiliation":[{"name":"Research Institute of Animal Embryo Technology Shahrekord University  Shahrekord Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehran","family":"Arabi","sequence":"additional","affiliation":[{"name":"Department of Animal Physiology Faculty of Basic Sciences Shahrekord University  Shahrekord Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4752-1722","authenticated-orcid":false,"given":"Naser","family":"Shams Esfandabadi","sequence":"additional","affiliation":[{"name":"Research Institute of Animal Embryo Technology Shahrekord University  Shahrekord Iran"},{"name":"Department of Clinical Sciences Faculty of Veterinary Medicine Shahrekord University  Shahrekord Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2986-2117","authenticated-orcid":false,"given":"Ebrahim","family":"Ahmadi","sequence":"additional","affiliation":[{"name":"Research Institute of Animal Embryo Technology Shahrekord University  Shahrekord Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azita","family":"Afzali","sequence":"additional","affiliation":[{"name":"Clinical Embryologist Shahrekord University of Medical Sciences  Shahrekord Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamidreza","family":"Ghanaei","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Shahrekord University  Shahrekord Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,29]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100\u2010736x2015000700002"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864\u2010022\u201009076\u20105"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0015\u20100282(97)81382\u20109"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40104\u2010024\u201001008\u20105"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/10495398.2015.1118383"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.1010305"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2003.08.016"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0090913"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2011.05.005"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2014.01.012"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1590\/1984\u20103143\u2010AR2021\u20100132"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.111.093252"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod54.3.563"},{"key":"e_1_2_11_15_1","doi-asserted-by":"crossref","unstructured":"Dorak M. 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