{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T16:41:15Z","timestamp":1790181675770,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,4,2]],"date-time":"2025-04-02T00:00:00Z","timestamp":1743552000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,4,2]],"date-time":"2025-04-02T00:00:00Z","timestamp":1743552000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    One Health is a unified approach that aims to sustain and maintain the health of humans, animals and the ecosystem. The environment plays an important role in the spread of resistance genes, as it is an unlimited source of antimicrobial resistance genes.\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    can acquire and spread resistance genes from its environment. Extended\u2010spectrum beta\u2010lactamase (ESBL)\u2010producing\n                    <jats:italic>E. coli<\/jats:italic>\n                    is a global concern because it can hydrolyse many beta\u2010lactam antibiotics. In this study, the aim was to isolate\n                    <jats:italic>E. coli<\/jats:italic>\n                    from faeces and soil samples collected from cattle, sheep and poultry in three districts (Golbas\u0131, Haymana and Cubuk) where livestock (cattle, sheep and poultry) farming activities are intensively carried out. In addition, the antibiotic susceptibility of the isolated\n                    <jats:italic>E. coli<\/jats:italic>\n                    was to be determined using phenotypic and genotypic methods and the presence of ESBLs was to be determined using a double\u2010disc synergy test. All 120\n                    <jats:italic>E. coli<\/jats:italic>\n                    isolates were confirmed by the MALDI\u2010TOF MS method. The resistance rates of all isolates were as follows: ampicillin, 12.5%; trimethoprim\/sulfamethoxazole, 6.6%; cefazolin, 0.83%; ciprofloxacin, 2.5%; ceftazidime, 0.83%; cefotaxime, 1.6% and ceftriaxone, 1.6%. Cefazolin (99.1%) and trimethoprim\/sulfamethoxazole (0.83%) were determined to have intermediate susceptibility. Only one\n                    <jats:italic>E. coli<\/jats:italic>\n                    strain was found to be ESBL positive via phenotypic methods, and whole\u2010genome analysis was performed on this strain. As a result of whole\u2010genome analysis, ESBL\u2010related\n                    <jats:italic>CTX\u2010M\u201014<\/jats:italic>\n                    and\n                    <jats:italic>TEM\u20101<\/jats:italic>\n                    genes were found in the plasmids. This is the first study on the determination of antibiotic susceptibility and the presence of ESBL in\n                    <jats:italic>E. coli<\/jats:italic>\n                    isolated from the soil and faeces samples of farms in these regions. More studies are needed to determine and understand antibiotic resistance and ESBL positivity in environmental samples. Therefore, the One Health approach should be emphasised.\n                  <\/jats:p>","DOI":"10.1002\/vms3.70209","type":"journal-article","created":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T08:26:16Z","timestamp":1743755176000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The Study of\n                    <i>Escherichia coli<\/i>\n                    as Antimicrobial\u2010Resistant Sentinel Microorganism Isolated in the Farms of Three Districts of Ankara by MALDI\u2010TOF MS and Genomic Analysis"],"prefix":"10.1002","volume":"11","author":[{"given":"Didem","family":"Karademir","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Microbiology Ankara University Graduate School of Health Science  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9722-4267","authenticated-orcid":false,"given":"Banu","family":"Kaskatepe","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Microbiology Ankara University Faculty of Pharmacy  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hilal Basak","family":"Erol","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Microbiology Ankara University Faculty of Pharmacy  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1434-2717","authenticated-orcid":false,"given":"Suleyman","family":"Yalcin","sequence":"additional","affiliation":[{"name":"Department of Microbiology Reference Laboratory and Biological Product MoH General Directorate of Public Health  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasemin Numanoglu","family":"Cevik","sequence":"additional","affiliation":[{"name":"Department of Microbiology Reference Laboratory and Biological Product MoH General Directorate of Public Health  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.disamonth.2020.100971"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2021.08.001"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579\u2010022\u201000779\u2010w"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2016-11741"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2010.03311.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01020"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00239-019-09914-3"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/tropicalmed4010022"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S234610"},{"issue":"8","key":"e_1_2_9_11_1","first-page":"985","article-title":"Molecular Characterization of Escherichia coli Isolates From Food Animals","volume":"54","author":"Debbarma S.","year":"2020","journal-title":"Indian Journal of Animal Research"},{"key":"e_1_2_9_12_1","volume-title":"European Committee on Antimicrobial Susceptibility Testing. 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