{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T06:46:18Z","timestamp":1790664378705,"version":"4.1.0"},"reference-count":64,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T00:00:00Z","timestamp":1738800000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Sperm membranes, rich in polyunsaturated fatty acids, are highly susceptible to lipid peroxidation. Oxidative stress significantly impacts sperm function, and B\u2010group vitamins play a crucial role in mitigating this damage.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study investigated the effects of vitamins B1 (VitB1) and B12 (VitB12) on sperm quality and seminal antioxidant status following cryopreservation in Arabi rams.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Semen was collected from 10 Arabi rams and pooled. The pooled semen was diluted and cryopreserved with varying concentrations of VitB1 and VitB12 (0, 0.5, 1, 2 and 4\u00a0mg\/mL). Post\u2010thaw sperm quality parameters, including motility, viability, membrane integrity and morphology, were assessed. In addition, seminal antioxidant enzyme activities and lipid peroxidation were evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Supplementation with 1\u00a0mg\/mL VitB12 and 0.5\u20131\u00a0mg\/mL VitB1 significantly enhanced post\u2010thaw sperm motility, viability, membrane integrity and morphology. This was accompanied by a reduction in malondialdehyde (MDA) levels. The highest total antioxidant capacity (TAC) was observed with 1\u00a0mg\/mL VitB12 and 0.5\u00a0mg\/mL VitB1 (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05), positively correlating with sperm motility and viability (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.01). Glutathione peroxidase (GPx) activity was highest in the 1\u00a0mg\/mL VitB12 and VitB1 groups and negatively correlated with sperm abnormalities (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.01). Catalase (CAT) activity significantly increased in the 1\u20132\u00a0mg\/mL VitB12 and 0.5\u20132\u00a0mg\/mL VitB1 groups, positively correlating with sperm progressive motility and viability (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.01).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These findings demonstrate that incorporating 1\u00a0mg\/mL VitB12 and 0.5\u00a0mg\/mL VitB1 into the cryopreservation extender effectively mitigates oxidative stress, enhances antioxidant defences and improves post\u2010thaw semen quality in Arabi rams.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70241","type":"journal-article","created":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T09:33:23Z","timestamp":1738834403000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Protective Effect of B1 and B12 Vitamins on Post\u2010Thaw Sperm Quality Parameters and Seminal Plasma Antioxidant Status in Rams"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1598-7154","authenticated-orcid":false,"given":"Saleh","family":"Tabatabaei Vakili","sequence":"first","affiliation":[{"name":"Department of Animal Science Faculty of Animal Science and Food Technology Agricultural Sciences and Natural Resources University of Khuzestan  Mollasani Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roghayeh","family":"Zeidi","sequence":"additional","affiliation":[{"name":"Department of Animal Science Faculty of Animal Science and Food Technology Agricultural Sciences and Natural Resources University of Khuzestan  Mollasani Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robab","family":"Nabhani","sequence":"additional","affiliation":[{"name":"Department of Animal Science Faculty of Animal Science and Food Technology Agricultural Sciences and Natural Resources University of Khuzestan  Mollasani Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,6]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2021.106761"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3109\/01480545.2013.851686"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10561\u2010015\u20109506\u20109"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.5897\/AJB11.4060"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.5897\/JCAB2017.0450"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom7020042"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.29219\/fnr.v67.8626"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2016.10.013"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.5653\/cerm.2022.05736"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2008.06.010"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939\u20104640.2003.tb02730.x"},{"key":"e_1_2_11_13_1","first-page":"156","article-title":"Improvement of Antioxidant Status and Semen Quality by Oral Supplementation With Vitamins C and E in Rams","volume":"26","author":"Cofr\u00e9\u2010Narbona E. 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