{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:33:37Z","timestamp":1791254017388,"version":"4.1.0"},"reference-count":55,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T00:00:00Z","timestamp":1739923200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004391","name":"China Medical University Hospital","doi-asserted-by":"publisher","award":["DMR\u2010112\u2010032"],"award-info":[{"award-number":["DMR\u2010112\u2010032"]}],"id":[{"id":"10.13039\/501100004391","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                      liver abscess (KLA) is an invasive disease, and the occurrence of infection is related to its virulence factors and colonization of the host's gastrointestinal (GI) tract. Some animal\u2010sourced isolates share virulence factors with human pathogens. However, the potential of\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      as a zoonotic agent has not been confirmed in murine infection model.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      To identify the prevalence and virulence profiles of\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      colonization in companion and wild animals and subsequently determine the pathogenicity of selected strains.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Forty\u2010five\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      isolates (45\/302) were obtained from faeces of companion or wild animals. Virulence factors,\n                      <jats:italic>gyrA<\/jats:italic>\n                      polymerase chain reaction with the restriction fragment length polymorphism (PCR\u2010RFLP) and pulsed field gel electrophoresis (PFGE) were detected and compared with our previous collection of 60 human pathogens. For KLA model and cytotoxicity test, three animal\u2010sourced isolates, CHKP0009 (snake, K1, KpII), CHKP0021 (turtle, K2, pLVPK, KpI, cluster I) and CHKP1027 (dog, non\u2010K1\/K2, HV, KpI, cluster III), with similar genotype and\/or phenotype to human pathogens were selected and evaluated for their virulence with human hypervirulent\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      (hvKp) CG43S.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The prevalence of\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      was higher in companion than wild animals.\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      was primarily isolated from dogs, turtles and snakes. Some animal\u2010sourced isolates carried virulence factors and revealed phylogenetic relatedness with human pathogens. In KLA model, BALB\/c mice infected with snake isolate CHKP0009 and dog isolate CHKP1027 survived for 14 days but showed significant bacterial loads in the liver and spleen. Notably, the pet turtle isolate CHKP0021 presented comparable virulence with human hvKp CG43S and induced liver abscess formation. All three selected animal\u2010sourced isolates could colonize in the GI tract and possess cytotoxic ability. These findings demonstrated pathogenicity of the animal\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      isolates. In addition, the high prevalence of\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      in companion animals and some isolates with virulence profiles suggested animal\u2010sourced\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      has the zoonotic potential to cause human disease.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Animals are the natural hosts of zoonotic pathogens. Some animal\u2010sourced\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      isolates are not only pathogenic in vivo but also exhibit phylogenetic relatedness to human pathogens, suggesting the existence of a zoonotic risk for\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      between these two populations.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70243","type":"journal-article","created":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T08:54:21Z","timestamp":1739955261000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Prevalence and Virulence Profiles of\n                    <i>Klebsiella pneumoniae<\/i>\n                    Isolated From Different Animals"],"prefix":"10.1002","volume":"11","author":[{"given":"Hsu\u2010Chung","family":"Liu","sequence":"first","affiliation":[{"name":"Division of Chest Medicine Department of Internal Medicine Cheng Ching Hospital  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui\u2010Ling","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology School of Medicine China Medical University  Taichung Taiwan"},{"name":"Institute of Medical Research China Medical University  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chien\u2010Shun","family":"Chiou","sequence":"additional","affiliation":[{"name":"Central Regional Laboratory Center for Diagnostics and Vaccine Development Centers for Disease Control  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Chun","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Life Sciences National Chung Hsing University  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming\u2010Ko","family":"Chiang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences National Chung Cheng University  Chia\u2010Yi Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwong\u2010Chung","family":"Tung","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine College of Veterinary Medicine National Chung Hsing University  Taichung Taiwan"},{"name":"Department of Veterinary Medicine National Chiayi University  Chiayi City Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Chyi","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Chung Shan Medical University Hospital  Taichung Taiwan"},{"name":"Department of Microbiology and Immunology School of Medicine Chung Shan Medical University  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min\u2010Chi","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology School of Medicine China Medical University  Taichung Taiwan"},{"name":"Division of Infectious Diseases Department of Internal Medicine China Medical University Hospital  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,19]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2020.101606"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/10766290260469651"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.59.5.1673\u20101682.1991"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11101357"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469\u20100691.2004.00973.x"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713\u201051\u20103\u2010915"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510310136"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2021.109307"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2004.05.008"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1991.00400080059010"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis565"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0022248"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.39.6.2351\u20102353.2001"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.14202\/vetworld.2021.1007\u20101013"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10071412"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2017.64"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku217"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1086\/519262"},{"issue":"2","key":"e_1_2_11_20_1","first-page":"95","article-title":"Bacteriological Culture of Blood From Critically Ill Neonatal Calves","volume":"38","author":"Fecteau G.","year":"1997","journal-title":"Canadian Veterinary Journal"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.00188"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1808.111053"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00034\u201011"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.11737"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11259\u2010021\u201009876\u20102"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.07.032"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/1749\u20104877.12637"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/2165316"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.592291"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2021.106354"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0096292"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkab118"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00483"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102180\u201011\u201050"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102180\u201012\u201013"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13099\u2010018\u20100266\u20105"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12941\u2010024\u201000711\u20105"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.14202\/vetworld.2020.1448\u20101456"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.4.589"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063870001200215"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0282245"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.575391"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.onehlt.2024.100904"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2019.108554"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917\u2010024\u201004222\u20105"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.00759\u201022"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096\u2010010\u20100915\u20101"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmii.2013.12.004"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564\u2010022\u201001263\u20100"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462\u20102920.16047"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.15212\/ZOONOSES\u20102023\u20100016"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1086\/516369"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.1989.195.12.1759"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2022.867784"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/2494913"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.642484"}],"container-title":["Veterinary Medicine and Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.70243","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T04:14:28Z","timestamp":1747196068000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/vms3.70243"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,19]]},"references-count":55,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["10.1002\/vms3.70243"],"URL":"https:\/\/doi.org\/10.1002\/vms3.70243","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/VMS3.70243\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70243\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70243\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70243\/v3\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70243\/v3\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70243\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70243\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70243\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70243\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70243\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70243\/v3\/response1","asserted-by":"object"}]},"ISSN":["2053-1095","2053-1095"],"issn-type":[{"value":"2053-1095","type":"print"},{"value":"2053-1095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,19]]},"assertion":[{"value":"2024-07-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70243"}}