{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T10:17:20Z","timestamp":1753870640751,"version":"3.41.2"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T00:00:00Z","timestamp":1742428800000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004410","name":"T\u00fcrkiye Bilimsel ve Teknolojik Ara\u015ftirma Kurumu","doi-asserted-by":"publisher","award":["122O764"],"award-info":[{"award-number":["122O764"]}],"id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Echinococcus granulosus<\/jats:italic> is a zoonotic disease that is widespread worldwide.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study aimed to determine the genetic diversity of <jats:italic>E. granulosus<\/jats:italic> isolates in sheep. Partial mitochondrial and nuclear gene sequences were used to determine intraspecific variation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>For this purpose, 41 sheep hydatid cyst isolates were collected from slaughterhouses in Elazig and Bingol provinces of T\u00fcrkiye. Following genomic DNA isolation from the germinal membrane of the cysts, PCR amplification and subsequent DNA sequence analysis were performed using primers that amplify mitochondrial <jats:italic>CO1<\/jats:italic>, <jats:italic>Nad5<\/jats:italic>, and nuclear <jats:italic>ef1\u03b1<\/jats:italic> gene regions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>DNA sequence analyses of mt\u2010<jats:italic>CO1<\/jats:italic> (1603\u00a0bp) and mt\u2010<jats:italic>Nad5<\/jats:italic> (625\u00a0bp) gene regions showed that 39 out of 41 isolates were identified as G1 and two isolates as G3. When the phylogenies formed by the sequences belonging to different gene regions were evaluated; in the phylogenetic tree created with the mt\u2010<jats:italic>CO1<\/jats:italic> and <jats:italic>Nad5<\/jats:italic> gene dataset, G1 and G3 isolates were in separate clades with the reference sequences, while in the phylogenetic tree created with the nuclear gene region <jats:italic>ef1\u03b1<\/jats:italic> dataset, the isolate belonging to the G3 genotype (ef1a.01) formed a sister clade with the G1 genotypes and the reference sequence. According to the haplotype network results obtained, 14 haplotypes, 15 haplotypes, and two haplotypes were determined for mt\u2010<jats:italic>CO1<\/jats:italic>, mt\u2010<jats:italic>Nad5<\/jats:italic>, and <jats:italic>ef1\u03b1<\/jats:italic> gene regions, respectively. Haplotype analysis of mt\u2010<jats:italic>CO1<\/jats:italic> and mt\u2010<jats:italic>Nad5<\/jats:italic> gene regions revealed high haplotype and low nucleotide diversity. Low nucleotide diversity was detected, and two haplotypes were determined as a result of haplotype analysis <jats:italic>ef1\u03b1<\/jats:italic> (1343\u00a0bp) gene.<\/jats:p><\/jats:sec>","DOI":"10.1002\/vms3.70313","type":"journal-article","created":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T08:11:34Z","timestamp":1742458294000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Analysis of Nuclear (<i>Ef1\u03b1<\/i>) and Mitochondrial (mt\u2010<i>CO1<\/i> and mt\u2010<i>Nad5<\/i>) Markers for Molecular Characterization of Sheep Isolates of Echinococcus granulosus sensu lato"],"prefix":"10.1002","volume":"11","author":[{"given":"Seyma","family":"Gunyakti Kilinc","sequence":"first","affiliation":[{"name":"Department of Parasitology Faculty of Veterinary Medicine Bingol University Bingol Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harun Kaya","family":"Kesik","sequence":"additional","affiliation":[{"name":"Department of Parasitology Faculty of Veterinary Medicine Bingol University Bingol Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Figen","family":"Celik","sequence":"additional","affiliation":[{"name":"Department of Parasitology Faculty of Veterinary Medicine Firat University Elazig Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3567-326X","authenticated-orcid":false,"given":"Sami","family":"Simsek","sequence":"additional","affiliation":[{"name":"Department of Parasitology Faculty of Veterinary Medicine Firat University Elazig Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,3,20]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-021-07156-1"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.2005.02655.x"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ecolsys.36.091704.175513"},{"key":"e_1_2_10_5_1","doi-asserted-by":"crossref","first-page":"123","DOI":"10.4274\/tpd.galenos.2019.6117","article-title":"Comparative Genotyping of Echinococcus granulosus Infecting Livestock in Turkey and Iran","volume":"43","author":"Barazesh A.","year":"2019","journal-title":"Turkish Journal of Parasitology"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(01)00357-5"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/trstmh\/tru138"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2014.10.005"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(92)90109-W"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(93)90065-7"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2024.107124"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2020.109132"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crpvbd.2022.100094"},{"key":"e_1_2_10_14_1","doi-asserted-by":"crossref","first-page":"399","DOI":"10.33899\/ijvs.2023.142564.3185","article-title":"Hydatid Cysts Strains Identification by Mitochondrial Dehydrogenase NADH Subunit5 Isolated From Cattle and Buffalo Host in Babylon Governorate, Iraq","volume":"38","author":"Farhood A. 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