{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T10:04:15Z","timestamp":1778493855756,"version":"3.51.4"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T00:00:00Z","timestamp":1744329600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T00:00:00Z","timestamp":1744329600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Bordetella bronchiseptica<\/jats:italic>\n                      is an essential bacterial pathogen characterized by chronic respiratory disease in dogs known as Kennel cough. The presence of causative antibodies in animals can also be detected by lipopolysaccharide antigen\u2010based enzyme linked immunosorbent assay (ELISA). In recent years, it has been determined that there is a significant relationship between acute phase proteins and diseases, and disease follow\u2010up can be done within the framework of this relationship.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In this study, blood sera from 150 dogs in an animal shelter in Van province were evaluated for\n                      <jats:italic>B. bronchiseptica<\/jats:italic>\n                      by the homemade ELISA method, and their correlations with serum amyloid A (SAA) were investigated. Blood serum samples were analysed for antibodies against\n                      <jats:italic>B. bronchiseptica<\/jats:italic>\n                      using a homemade ELISA method. Positive animals were also molecularly confirmed using nasal swabs by PCR. A commercial ELISA kit determined SAA levels in blood sera.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Eighteen (12%) of the analysed blood serum samples were found positive by the homemade ELISA method. SAA concentrations in the positive blood sera were elevated from 12.7 to \u226438.98\u00a0mg\/L. SAA concentrations in blood sera serologically positive for\n                      <jats:italic>B. bronchiseptica<\/jats:italic>\n                      were statistically significant.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      In this study, in which the relationship between SAA concentration and\n                      <jats:italic>B. bronchiseptica<\/jats:italic>\n                      was investigated for the first time in Turkey, it was concluded that SAA concentration analysis may help diagnose and monitor the disease. In addition, the presence and prevalence of this critical and zoonotic agent causing chronic respiratory tract disease in dogs in Van province was revealed for the first time in this study.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70323","type":"journal-article","created":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T07:31:12Z","timestamp":1744356672000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Investigation of the Correlation Between ELISA and Serum Amyloid A in the Diagnosis of\n                    <i>Bordetella bronchiseptica<\/i>\n                    in Dogs"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0894-7357","authenticated-orcid":false,"given":"Kadir","family":"Akar","sequence":"first","affiliation":[{"name":"Department of Microbiology Faculty of Veterinary Medicine Van Yuzuncu Yil University  Van T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9950-330X","authenticated-orcid":false,"given":"G\u00f6k\u00e7enur","family":"Sanio\u011flu G\u00f6len","sequence":"additional","affiliation":[{"name":"Department of Microbiology Faculty of Veterinary Medicine Aksaray University  Aksaray T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u0130smail Hakk\u0131","family":"Ekin","sequence":"additional","affiliation":[{"name":"Department of Microbiology Faculty of Veterinary Medicine Van Yuzuncu Yil University  Van T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,4,11]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2022.e11330"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jambio\/lxad200"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003\u20102697(76)90527\u20103"},{"key":"e_1_2_12_5_1","first-page":"11","article-title":"Exposure to Infectious Agents in Dogs in Remote Coastal British Columbia: Possible Sentinels of Diseases in Wildlife and Humans","volume":"75","author":"Bryan H. 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