{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T01:04:55Z","timestamp":1774314295591,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,4,2]],"date-time":"2025-04-02T00:00:00Z","timestamp":1743552000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,4,2]],"date-time":"2025-04-02T00:00:00Z","timestamp":1743552000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005421","name":"Guilan University of Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005421","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Echinococcus granulosus<\/jats:italic>\n                      sensu lato is one of the most important zoonotic parasites in many regions of the world. Cystic echinococcosis (CE) is a major economic and public health challenge in Iran.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      This study aimed to determine the prevalence and genotypes of\n                      <jats:italic>Echinococcus granulosus<\/jats:italic>\n                      isolates from slaughtered buffaloes in Guilan Province, northern Iran.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We examined 384 buffaloes at the Talesh county abattoir, isolating 42 hydatid cysts. We sequenced the mitochondrial cytochrome c oxidase subunit 1 (CO1) and NADH dehydrogenase subunit 1 (ND1) genes for genotype analysis. Molecular phylogeny and haplotype network analysis was performed for the isolates.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Our results showed that 10.9% of the buffaloes were infected with hydatid cysts, with 90.5% of infections in the liver and 9.5% in the lungs. Only one liver cyst was fertile; the rest were non\u2010fertile. Amongst the infected animals, 21.4% (9\/42) were male, and 78.6% (33\/42) were female. No significant gender\u2010related difference in infection rates was observed (\n                      <jats:italic>p<\/jats:italic>\n                      = 0.057); however, a significant relationship was found between age groups and infection (\n                      <jats:italic>p<\/jats:italic>\n                      = 0.030). Sequence analysis identified all isolates as belonging to the G1 genotype of\n                      <jats:italic>E. granulosus<\/jats:italic>\n                      , with five haplotypes being identified.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The study highlights the high prevalence of hydatid cysts in buffaloes, though fertility was low, suggesting a minor role for buffaloes in CE epidemiology in this region. Similar to other studies, our research shows that infection rates increase significantly with age.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70328","type":"journal-article","created":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T07:43:52Z","timestamp":1743752632000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Frequency and Sequence Analysis of\n                    <i>Echinococcus granulosus<\/i>\n                    Sensu Lato Isolated From Buffaloes in Northern Iran"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-9194-9412","authenticated-orcid":false,"given":"Fariborz","family":"Ghanbari","sequence":"first","affiliation":[{"name":"Department of Medical Parasitology and Mycology School of Medicine, Guilan University of Medical Sciences  Rasht Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1742-1245","authenticated-orcid":false,"given":"Keyhan","family":"Ashrafi","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology and Mycology School of Medicine, Guilan University of Medical Sciences  Rasht Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3257-5389","authenticated-orcid":false,"given":"Majid","family":"Fasihi Harandi","sequence":"additional","affiliation":[{"name":"Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences  Kerman Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0032-1635","authenticated-orcid":false,"given":"Somayeh","family":"Abbaszadeh","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology and Mycology School of Medicine, Guilan University of Medical Sciences  Rasht Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3791-8451","authenticated-orcid":false,"given":"Asieh","family":"Ashouri","sequence":"additional","affiliation":[{"name":"Research Center of Health and Environment, School of Health, Guilan University of Medical Sciences  Rasht Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2159-4066","authenticated-orcid":false,"given":"Elham","family":"Hajialilo","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology and Mycology School of Medicine, Qazvin University of Medical Sciences  Qazvin Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2686-7748","authenticated-orcid":false,"given":"Meysam","family":"Sharifdini","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology and Mycology School of Medicine, Guilan University of Medical Sciences  Rasht Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,4,2]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436\u2010010\u20102170\u2010x"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12639\u2010024\u201001738\u20103"},{"issue":"1","key":"e_1_2_11_4_1","first-page":"83","article-title":"Molecular Characterization of a New Microvariant of the G3 Genotype for Echinococcus granulosus in Water Buffalo in Iran","volume":"6","author":"Babazadeh M.","year":"2015","journal-title":"Veterinary Research Forum"},{"issue":"3","key":"e_1_2_11_5_1","first-page":"e249","article-title":"Prevalence and Intensity Study of Hydatidosis in Slaughtered Buffaloes in Urmia City","volume":"64","author":"Basti A. 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