{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T17:01:16Z","timestamp":1775322076639,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,4,26]],"date-time":"2025-04-26T00:00:00Z","timestamp":1745625600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,4,26]],"date-time":"2025-04-26T00:00:00Z","timestamp":1745625600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Neospora caninum<\/jats:italic>\n                      is an obligate intracellular protozoan that is well established as a causative agent of abortion in dairy cattle worldwide.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      The objective of this study was to determine the role of\n                      <jats:italic>N. caninum<\/jats:italic>\n                      infection in the abortion in dairy cattle in the Khorasan Razavi Province, Iran.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>From 2022 to 2024, 105 aborted bovine foetuses were collected from dairy cattle in Khorasan Razavi province. Brain samples of aborted foetuses were tested using nested PCR and histopathological examination. In addition, blood samples were collected from dairy cattle that had aborted PCR\u2010positive foetuses and were analysed using enzyme\u2010linked immunosorbent assay (ELISA).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results and Conclusions<\/jats:title>\n                    <jats:p>\n                      In the present study,\n                      <jats:italic>N. caninum<\/jats:italic>\n                      infection was detected in 24.76% (26 out of 105) of aborted bovine foetuses by nested PCR analysis. The brain tissues of 20 bovine\u2010aborted foetuses were only suitable for histopathological examination. Lesions of the central nervous system were severe hyperaemia, perivascular cuffing, astrogliosis, mild encephalitis and focal necrosis. One foetus exhibited a 32\u2010\u00b5m\n                      <jats:italic>N. caninum<\/jats:italic>\n                      cyst within the brain tissue. IgG antibodies against\n                      <jats:italic>N. caninum<\/jats:italic>\n                      were identified in all dairy cattle that aborted infected foetuses through ELISA testing. Molecular, histopathological and serological findings strongly suggest that\n                      <jats:italic>N. caninum<\/jats:italic>\n                      plays a significant role in bovine abortion in dairy cattle in Khorasan Razavi Province, northeast Iran.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70329","type":"journal-article","created":{"date-parts":[[2025,4,26]],"date-time":"2025-04-26T05:59:46Z","timestamp":1745647186000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Pathological and Molecular Studies of\n                    <i>Neospora caninum<\/i>\n                    Infection in Aborted Bovine Foetuses in Khorasan Razavi Province, Iran"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-6265-4837","authenticated-orcid":false,"given":"Asma 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