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Blood and colostrum samples were collected within the first 6\u00a0h following birth from 24 pregnant Awassi sheep. On the basis of the determined colostrum IgG levels, low (Low IgG [LIGG], 44.83\u00a0\u00b1\u00a04.97\u00a0mg\/mL) and high (High IgG [HIGG], 80.71\u00a0\u00b1\u00a03.31\u00a0mg\/mL) groups were formed. In addition to measuring colostrum compositional parameters, expressions of miR\u201029b and miR\u2010148a were determined in plasma and colostrum. Although somatic cell score (SCS), pH and electrical conductivity (EC) were higher, fat\u2010free dry matter (FFDM), protein, lactose and freezing point were lower in HIGG group. Compared to LIGG, miR\u201029b was downregulated approximately 5\u2010fold in the HIGG, whereas miR\u2010148a was downregulated more than 3\u2010fold in colostrum. There were negative correlations between colostrum miR\u2010148a and colostrum IgG, SCS and pH. Colostrum IgG was positively correlated with pH and freezing point and negatively correlated with FFDM, protein and lactose. Area under the curve (AUC) values of SCS, pH, FFDM, protein, lactose, freezing point, EC and miR\u2010148a (colostrum) were significant. Sensitivity and specificity of pH were 100% and 66.7%, of SCS were 81.8% and 66.7% and of miR\u2010148a (Colostrum) were 75% and 75% in HIGG and LIGG. According to the results, miR\u201029b might be an important molecular target for lamb development, whereas miR\u2010148a could be a potential biomarker for colostrum quality. Moreover, the study showed that SCS and pH might be useful diagnostic parameters for IgG status because of higher sensitivity rates and AUC values.<\/jats:p>","DOI":"10.1002\/vms3.70335","type":"journal-article","created":{"date-parts":[[2025,4,5]],"date-time":"2025-04-05T14:16:08Z","timestamp":1743862568000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Expression Patterns of miR\u201029b and miR\u2010148a in Colostrum of Awassi Sheep With Higher Immunoglobulin G Levels"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5753-8985","authenticated-orcid":false,"given":"H\u00fcseyin","family":"\u00d6zkan","sequence":"first","affiliation":[{"name":"Department of Genetics Faculty of Veterinary Medicine Hatay Mustafa Kemal University  Antakya Hatay Turkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4805-0993","authenticated-orcid":false,"given":"Ufuk","family":"Kaya","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, Faculty of Veterinary Medicine Hatay Mustafa Kemal University  Antakya Hatay Turkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hasan H\u00fcseyin","family":"Ke\u00e7eli","sequence":"additional","affiliation":[{"name":"Department of Genetics Faculty of Veterinary Medicine Hatay Mustafa Kemal University  Antakya Hatay Turkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramazan","family":"Sertkol","sequence":"additional","affiliation":[{"name":"Department of Veterinary Obstetrics, Institute of Health Sciences Gynecology, and Artificial Insemination Hatay Mustafa Kemal University  Antakya Hatay Turkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mustafa Kemal","family":"Saribay","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine Hatay Mustafa Kemal University  Antakya Hatay Turkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,4,4]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022\u20100302(80)82959\u20106"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.22207\/JPAM.16.2.59"},{"issue":"6","key":"e_1_2_10_4_1","first-page":"3706","article-title":"Colostrum Composition of Santa In\u00eas Sheep and Passive Transfer of Immunity to Lambs","volume":"98","author":"Alves A. 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