{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T03:15:50Z","timestamp":1791170150898,"version":"4.1.0"},"reference-count":71,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T00:00:00Z","timestamp":1745798400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T00:00:00Z","timestamp":1745798400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Ram semen cryotolerance problem continues despite intensive research. This study aimed to evaluate the effect of replacing total seminal plasma (SP) with that of high\u2010cryotolerant rams or extender on semen quality parameters in low\u2010cryotolerant rams. Rams previously determined as low (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a06) and high\u2010cryotolerant (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a05) were used in the breeding season. High\u2010cryotolerant rams were only included with their SP in the SP+ group. Each normospermic fresh ejaculate from low\u2010cryotolerant rams was split into three aliquots to form the groups. The control aliquot was diluted using a two\u2010step process. Centrifugation removed SP from the second aliquot (SP\u2212 group), and the sperm pellet was rediluted with a Tris\u2010based extender using the same amount of SP removed. The third aliquot's SP was removed as in the SP\u2212 group, but the remaining sperm pellet was rediluted with fresh SP harvested from high\u2010cryotolerant rams using the same amounts of removed SP in the SP+ group. Semen was rediluted with a Tris\u2010based extender in the SP\u2212 and SP+ groups using a two\u2010step process as in the control group. Sperm quality was similar between the groups after dilution and equilibration. However, the post\u2010thaw semen quality was higher in the SP\u2212 (one parameter) and SP+ (four parameters) groups than in the control group (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.05). Additionally, these parameters were similar in the SP\u2212 and SP+ groups. This study showed that, although an adequate extender had positive effects, replacing the whole SP with that of high\u2010cryotolerant rams may better solve the low cryotolerance problem in ram semen.\n                  <\/jats:p>","DOI":"10.1002\/vms3.70381","type":"journal-article","created":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T13:47:04Z","timestamp":1745848024000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Improving Ram Semen Low Cryotolerance by Replacing the Seminal Plasma With That of High\u2010Cryotolerant Rams or Extender"],"prefix":"10.1002","volume":"11","author":[{"given":"Cemal","family":"Dayan\u0131kl\u0131","sequence":"first","affiliation":[{"name":"Department of Breeding Techniques Sheep Breeding Research Institute  Bal\u0131kesir T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7130-8796","authenticated-orcid":false,"given":"B\u00fclent","family":"B\u00fclb\u00fcl","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination Faculty of Veterinary Medicine Dokuz Eyl\u00fcl University  \u0130zmir T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u015e\u00fckr\u00fc","family":"Do\u011fan","sequence":"additional","affiliation":[{"name":"Department of Animal Breeding Bahri Da\u011fda\u015f International Agricultural Research Institute  Konya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ebru","family":"\u015eeng\u00fcl","sequence":"additional","affiliation":[{"name":"Department of Breeding Techniques Sheep Breeding Research Institute  Bal\u0131kesir T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mesut","family":"K\u0131rba\u015f","sequence":"additional","affiliation":[{"name":"Department of Animal Breeding Bahri Da\u011fda\u015f International Agricultural Research Institute  Konya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yavuz","family":"Kal","sequence":"additional","affiliation":[{"name":"Department of Animal Breeding Bahri Da\u011fda\u015f International Agricultural Research Institute  Konya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehmet Bozkurt","family":"Ataman","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination Faculty of Veterinary Medicine Sel\u00e7uk University  Konya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,28]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2014.02.013"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093\u2010691X(00)00251\u2010X"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2015.08.019"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.13555"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2014.09.004"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2021.106318"},{"key":"e_1_2_10_8_1","first-page":"220","article-title":"Artificial Insemination Techniques and Physiopathological Implications in Sheep","volume":"9","author":"Alvares C. 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