{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T13:13:56Z","timestamp":1774271636673,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T00:00:00Z","timestamp":1747094400000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Neonatal mortality in dogs is high, ranging from approximately 5% to 35%, yet investigations into its causes and risk factors remain scarce. Postmortem examination is a crucial tool for identifying the underlying causes of neonatal death and improving disease diagnosis. However, the anatomical, physiological and histological differences between neonates and adults present unique challenges for necropsy procedures. Establishing a standardised neonatal necropsy protocol is essential for accurately determining the causes of death and associated diseases. This pilot study aimed to standardise a necropsy technique for canine neonates. During the examination, the spleen, heart, lungs, tongue, stomach, intestines, liver, pancreas, kidneys, bladder, brain and bones were collected from nine neonatal canines. The method involved monoblock organ removal, followed by separation on the basis of the standard organisation used in adult necropsies. Histological staining and analysis revealed structural differences between neonatal and adult tissues. Neonatal hepatocytes presented a more basophilic cytoplasm, suggesting high metabolic activity, whereas gastric glands and parietal cells were less developed, indicating ongoing maturation. Additionally, the pancreas, lungs, kidneys and spleen displayed morphological characteristics consistent with those of the early development stages. Understanding these particularities may enhance veterinary diagnostic and clinical approaches, contribute to the development of preventive measures and ultimately reduce neonatal mortality in dogs. The standardisation of necropsy protocols facilitates the recognition of disease patterns, improves pathological documentation and supports further research on neonatal canine mortality.<\/jats:p>","DOI":"10.1002\/vms3.70392","type":"journal-article","created":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:50:12Z","timestamp":1747191012000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Necropsy Technique and Histological Characterisation of Organs From Neonatal Puppies: What Do We Know?"],"prefix":"10.1002","volume":"11","author":[{"given":"Vict\u00f3ria","family":"Ghedin","sequence":"first","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP) School of Veterinary Medicine and Animal Science Department of Veterinary Clinics  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00e9ssica Cardia","family":"de Melo","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP) School of Veterinary Medicine and Animal Science Department of Veterinary Clinics  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8153-2038","authenticated-orcid":false,"given":"Fernanda Barthelson","family":"Carvalho de Moura","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP) School of Veterinary Medicine and Animal Science Department of Veterinary Clinics  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keylla Helena Nobre","family":"Pac\u00edfico Pereira","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP) School of Veterinary Medicine and Animal Science Department of Veterinary Clinics  Botucatu Brazil"},{"name":"Federal University of Alagoas (UFAL) Department of Veterinary Medicine  Vi\u00e7osa \u2013 Alagoas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos Mario","family":"Gonzalez Zambrano","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP) Faculty of Medicine, Department of Pathology  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliana Jurado","family":"Jim\u00e9nez","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP) Faculty of Medicine, Department of Pathology  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela Abreu","family":"Botelho","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP) Faculty of Medicine, Department of Pathology  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu\u00edz Guilherme","family":"Decore Benevenuto","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP) School of Veterinary 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