{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T13:15:12Z","timestamp":1774271712928,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T00:00:00Z","timestamp":1749513600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T00:00:00Z","timestamp":1749513600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Neospora caninum<\/jats:italic>\n                      is one of the most significant causative agents of abortion in dairy cattle. Canids, especially dogs, act as definitive hosts and cattle serve as intermediate hosts for these coccidian protozoa, which can cause neuromuscular disease in dogs. The objective of this study was to determine the molecular characterisation of\n                      <jats:italic>N. caninum<\/jats:italic>\n                      in different tissues of dogs and cats in Shiraz, Southern Iran.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A total of 684 samples were collected from various tissues of 28 dogs and 29 cats during 2015\u20132016. Genomic DNA from the 684 samples was extracted manually using the phenol\u2010chloroform method, and the NC5 gene was amplified by polymerase chain reaction (PCR), using specific primers for\n                      <jats:italic>Neospora<\/jats:italic>\n                      (Np21plus and Np6plus). Subsequently, PCR products from 4 samples were sequenced. BioEdit software was utilised to align the sequences and compare them to previously published sequences in GenBank. Phylogenetic analysis was conducted using the Mega 5.0 software and the maximum likelihood method was employed to conduct phylogenetic analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Four out of 684 samples (0.58%) tested positive when compared with controls (2 samples from duodenum and ileum tissues of 2 dogs and 2 samples from the same tissues of 2 cats). All positive samples were confirmed as\n                      <jats:italic>N. caninum<\/jats:italic>\n                      through analysis of the NC5 gene sequence. Sequence comparison revealed no differences among the nucleotide sequences of the\n                      <jats:italic>N. caninum<\/jats:italic>\n                      isolates. Additionally, the sequences of\n                      <jats:italic>N. caninum<\/jats:italic>\n                      were identical and showed 100% similarity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      This study confirmed the presence of\n                      <jats:italic>N. caninum<\/jats:italic>\n                      in dogs and cats in rural regions of Shiraz, the capital of Fars province, Southern Iran. Further studies are recommended to expand on the findings of this study regarding neosporosis in this area.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70419","type":"journal-article","created":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T13:24:14Z","timestamp":1749561854000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Detection of Neospora Caninum in Different Tissues of Dogs and Cats in Shiraz, Iran"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7807-4793","authenticated-orcid":false,"given":"Gholam Reza","family":"Hatam","sequence":"first","affiliation":[{"name":"Basic Sciences in Infectious Diseases Research Center Shiraz University of Medical Sciences  Shiraz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6006-5485","authenticated-orcid":false,"given":"Fattaneh","family":"Mikaeili","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology, School of Medicine Shiraz University of Medical Sciences  Shiraz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7945-2370","authenticated-orcid":false,"given":"Qasem","family":"Asgari","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology, School of Medicine Shiraz University of Medical Sciences  Shiraz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5923-5694","authenticated-orcid":false,"given":"Mehdi","family":"Namavari","sequence":"additional","affiliation":[{"name":"4\u2010 Mehdi Namavari, Shiraz Branch, Razi Vaccine and Serum Research Institute Agricultural Research, Education and Extension Organization (AREEO)  Shiraz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-0612-5721","authenticated-orcid":false,"given":"Shamsi","family":"Noorpisheh Ghadimi","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology, School of Allied Medicine Sciences Fasa University of Medical Sciences  Fasa Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,6,10]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-4758(99)01604-X"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1645\/0022-3395(2001)087[0906:PONCII]2.0.CO;2"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1645\/0022-3395(2001)087[0612:FIONCF]2.0.CO;2"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.146.22.649"},{"issue":"2","key":"e_1_2_11_6_1","first-page":"185","article-title":"Forouzandeh\u2010Moghadam, M. 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