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Rectal swabs were collected for ESBL\u2010producing\n                      <jats:italic>E<\/jats:italic>\n                      .\n                      <jats:italic>coli<\/jats:italic>\n                      identification and detection. A questionnaire was used to collect pet data, and univariable and multivariable analysis was conducted to determine risk factors ESBL\u2010producing\n                      <jats:italic>E. coli<\/jats:italic>\n                      detection.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 160 rectal swabs were collected from physically healthy pets in the Klang Valley, with 6.8% (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a011) dogs and 3.8% (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a06) cats were positive for ESBL\u2010producing\n                      <jats:italic>E. coli<\/jats:italic>\n                      (10.6%). The highest resistance demonstrated by the ESBL isolates was towards ampicillin, cephalexin and cefotaxime (100%). Multiple\u2010drug resistance of the ESBL\u2010isolates was high, with 91.7% (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a022) and 68.8% (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a011) in dogs and cats. The most predominant ESBL genes was\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>CTX\u2010M<\/jats:sub>\n                      (43.2%). None of these isolates carried\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>SHV<\/jats:sub>\n                      . Dogs were four times more likely to carry ESBL\u2010producing\n                      <jats:italic>E. coli<\/jats:italic>\n                      in their faeces compared to cats. ESBL\u2010producing\n                      <jats:italic>E. coli<\/jats:italic>\n                      carriage was positively associated in dogs, among pets sharing feeding area or pets with a history of gastrointestinal symptoms.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      ESBL\u2010producing\n                      <jats:italic>E. coli<\/jats:italic>\n                      in cats and dogs in Malaysia was determined at 10.6%, with dogs at a greater risk of carrying the bacteria. Co\u2010resistance to more than three types of tested antibiotics for the ESBL\u2010producing\n                      <jats:italic>E. coli<\/jats:italic>\n                      isolated was high. Pets act as reservoirs for antimicrobial resistance (AMR) and resistance genes that can be shared with human through interactions. Antimicrobial stewardship remains critical to safeguard not only antibiotics but also the health of pets and humans.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70452","type":"journal-article","created":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T04:24:29Z","timestamp":1749788669000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Extended Spectrum Beta\u2010Lactamase Producing\n                    <i>Escherichia coli<\/i>\n                    in Pet Cats and Dogs in Central Peninsular Malaysia"],"prefix":"10.1002","volume":"11","author":[{"given":"Khaleeda Azalea","family":"Dzulkifli","sequence":"first","affiliation":[{"name":"Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine Universiti Putra Malaysia  Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Latiffah","family":"Hassan","sequence":"additional","affiliation":[{"name":"Department of Veterinary Laboratory Diagnostics, Faculty of Veterinary Medicine Universiti Putra Malaysia  Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zunita","family":"Zakaria","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine Universiti Putra Malaysia  Selangor Malaysia"},{"name":"Institute of Bioscience Universiti Putra Malaysia  Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Puteri Azaziah Megat Abdul","family":"Rani","sequence":"additional","affiliation":[{"name":"Jade Hills Veterinary Hospital, Jalan Jade Hills  Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1031-9915","authenticated-orcid":false,"given":"Nur Indah","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine Universiti Putra Malaysia  Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,13]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.6093\/2531-9906\/5261"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.4236\/ojmm.2020.104015"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12889\u2010016\u20103377\u20102"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11121730"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362\u2010028X.JFP\u201018\u2010352"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0187239"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.4103\/2231\u20100770.197508"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiph.2023.11.006"},{"key":"e_1_2_12_10_1","volume-title":"Manual of Antimicrobial Susceptibility Testing","author":"Cavalieri S. 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