{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T11:13:10Z","timestamp":1785928390131,"version":"3.56.0"},"reference-count":21,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T00:00:00Z","timestamp":1752105600000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100007356","name":"Fondation M\u00e9diterran\u00e9e Infection","doi-asserted-by":"publisher","award":["ANR\u201010\u2010IAHU\u201003"],"award-info":[{"award-number":["ANR\u201010\u2010IAHU\u201003"]}],"id":[{"id":"10.13039\/100007356","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    An\n                    <jats:italic>Enterobacter cloacae<\/jats:italic>\n                    ST48 isolate exhibiting a colistin\u2010heteroresistant phenotype was obtained from raw cow milk in Algeria. Two subpopulations were detected, both carrying the\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>TEM\u20101<\/jats:sub>\n                    gene. While one subpopulation (\n                    <jats:italic>E. cloacae<\/jats:italic>\n                    TS34\u2010a) was highly susceptible to colistin (MIC 0.06\u00a0\u00b5g\/mL), the other (\n                    <jats:italic>E. cloacae<\/jats:italic>\n                    TS34\u2010b) was resistant (MIC 8\u00a0\u00b5g\/mL). Population analysis profiling (PAP) confirmed heteroresistance. PCR amplification revealed the absence of\n                    <jats:italic>mcr<\/jats:italic>\n                    genes in both subpopulations. However, multiple non\u2010synonymous mutations, including a stop\u2010codon mutation in\n                    <jats:italic>phoP<\/jats:italic>\n                    gene, were found in the resistant subpopulation. This is the first report of colistin\u2010heteroresistant\n                    <jats:italic>E. cloacae<\/jats:italic>\n                    isolated from raw milk, underscoring the importance of vigilance in the dairy industry and food safety.\n                  <\/jats:p>","DOI":"10.1002\/vms3.70501","type":"journal-article","created":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T16:48:27Z","timestamp":1752166107000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Detection and Characterization of Colistin Heteroresistance in an\n                    <i>Enterobacter cloacae<\/i>\n                    ST48 Strain Isolated From Raw Milk in Algeria"],"prefix":"10.1002","volume":"11","author":[{"given":"Sedrati","family":"Tahar","sequence":"first","affiliation":[{"name":"Laboratoire de recherche Gestion des Ressources Animales Locales Ecole Nationale Sup\u00e9rieure V\u00e9t\u00e9rinaire  Oued Smar Alg\u00e9rie"},{"name":"Universit\u00e9 Mohamed El Bachir El Ibrahimi de Bordj Bou Arreridj  El Anceur Alg\u00e9rie"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Touati","family":"Abdelaziz","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Bejaia Laboratoire d'Ecologie Microbienne FSNV  B\u00e9ja\u00efa Alg\u00e9rie"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tennah","family":"Safia","sequence":"additional","affiliation":[{"name":"Laboratoire de recherche Gestion des Ressources Animales Locales Ecole Nationale Sup\u00e9rieure V\u00e9t\u00e9rinaire  Oued Smar Alg\u00e9rie"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5338-5552","authenticated-orcid":false,"given":"Menoueri M.","family":"Nabil","sequence":"additional","affiliation":[{"name":"Universit\u00e9 SAAD DAHLAB de BLIDA route de Soum\u00e2a BP 270 Blida  Blida Alg\u00e9rie"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6466-2324","authenticated-orcid":false,"given":"Seydina M.","family":"Diene","sequence":"additional","affiliation":[{"name":"MEPHI AP\u2010HM IHU\u2010M\u00e9diterran\u00e9e Infection Facult\u00e9 de Pharmacie Aix\u2010Marseille Univ.  Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,10]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/03079457.2018.1524573"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579\u2010019\u20100218\u20101"},{"key":"e_1_2_11_4_1","volume-title":"M\u00e9canismes de la R\u00e9sistance \u00e0 la Colistine Chez Les Bact\u00e9ries \u00e0 Gram N\u00e9gatif","author":"Baron S.","year":"2017"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1101\/307132"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkw260"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-07845-6"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-018-7449-0"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05055-14"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.12704"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2021.152861"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bsheal.2020.05.001"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20103\u2010030\u201016373"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2019.12.001"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13028-019-0486-9"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.02133"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00064-16"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics12071111"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00791"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiac.2018.09.004"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2018.0147"},{"issue":"2","key":"e_1_2_11_22_1","first-page":"e72","article-title":"Identification of Novel Mobile Colistin Resistance Gene Mcr\u201010 in Enterobacterales Isolates From Clinical and Environmental Samples","volume":"3","author":"Wang C.","year":"2022","journal-title":"Lancet Microbe"}],"container-title":["Veterinary Medicine and Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.70501","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/vms3.70501","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.70501","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T12:23:31Z","timestamp":1774268611000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/vms3.70501"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":21,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1002\/vms3.70501"],"URL":"https:\/\/doi.org\/10.1002\/vms3.70501","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/VMS3.70501\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70501\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70501\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70501\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70501\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70501\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70501\/v1\/review2","asserted-by":"object"}]},"ISSN":["2053-1095","2053-1095"],"issn-type":[{"value":"2053-1095","type":"print"},{"value":"2053-1095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"2025-04-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70501"}}