{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T18:53:48Z","timestamp":1787856828534,"version":"build-2784847793"},"reference-count":75,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T00:00:00Z","timestamp":1752796800000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Nanoparticles (NPs), such as green cobalt NPs (CoNPs), are easier to pass through cell membranes in animals and interact rapidly with biological systems. Therefore, using green CoNPs is one of the recommendations for enhancing the bioavailability of cobalt, thereby improving its absorption.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>The goal of this study was to explore the influences of biological nano\u2010cobalt (BNCo) as a feed supplement on broiler growth performance, haematology, blood chemistry, antioxidant activities, immunological status, digestive enzymes and carcass characteristics.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A total of 300 Arbour Acre broiler chicks, all unsexed, were distributed into 5 treatment groups, each containing 60 chicks, at random. Five replications of each group were formed, each containing 12 chicks. The first group received a control diet free of BNCo, whereas the second, third, fourth and fifth groups were administered diets fortified with BNCo at concentrations of 100, 200, 300 and 400\u00a0ppm, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The results illustrated a significant increase in body weight (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.001), body weight gain (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.001) and improvement in feed conversion ratio (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.01) in comparison to the control diet. BNCo supplementation significantly increased the percentage of immune organs, especially the spleen and thymus (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05,\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.01). Moreover, BNCo supplementation significantly increased haemoglobin (Hb) (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.01), red blood cells (RBCs) (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05) and white blood cells (WBCs) (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). In broiler chickens, BNCo supplementation increased serum levels of total protein, albumin and globulin significantly (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05) and reduced serum levels of aspartate aminotransferase (AST) activity (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.001) and alanine aminotransaminase (ALT) (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.01). The BNCo in feed supplementation raised blood levels of cobalt and markedly improved lipid markers, immunological and antioxidant status (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). Furthermore, digestive enzymes were significantly boosted (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05) by BNCo treatments.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The results indicated that BNCo supplementation at 200\u00a0ppm\/kg diet produced the best overall performance, demonstrating its potential as an innovative additive for broiler diets.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70508","type":"journal-article","created":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T08:03:41Z","timestamp":1752825821000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Application of Green Cobalt Nanoparticles in the Diet of Broiler Chickens to Improve Sustainable Production and Health"],"prefix":"10.1002","volume":"11","author":[{"given":"Fayiz M.","family":"Reda","sequence":"first","affiliation":[{"name":"Poultry Department Faculty of Agriculture Zagazig University Zagazig Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdullah S.","family":"Alawam","sequence":"additional","affiliation":[{"name":"Department of Biology College of Science Imam Mohammad Ibn Saud Islamic University (IMSIU) Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hemat K.","family":"Mahmoud","sequence":"additional","affiliation":[{"name":"Department of Animal Production Faculty of Agriculture Zagazig University Zagazig Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed T.","family":"El\u2010Saadony","sequence":"additional","affiliation":[{"name":"Department of Agricultural Microbiology Faculty of Agriculture Zagazig University Zagazig Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayman S.","family":"Salah","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Clinical Nutrition Faculty of Veterinary Medicine New Valley University El\u2010Kharga Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hassan A.","family":"Rudayni","sequence":"additional","affiliation":[{"name":"Department of Biology College of Science Imam Mohammad Ibn Saud Islamic University (IMSIU) Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed A.","family":"Allam","sequence":"additional","affiliation":[{"name":"Department of Biology College of Science Imam Mohammad Ibn Saud Islamic University (IMSIU) Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karima","family":"El\u2010Naggar","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Veterinary Clinical Nutrition Faculty of Veterinary Medicine Alexandria University Alexandria Governorate Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8020-0971","authenticated-orcid":false,"given":"Mahmoud","family":"Alagawany","sequence":"additional","affiliation":[{"name":"Poultry Department Faculty of Agriculture Zagazig University Zagazig Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7214-3774","authenticated-orcid":false,"given":"Most","family":"Khairunnesa","sequence":"additional","affiliation":[{"name":"Department of Dairy and Poultry Science Faculty of Veterinary Medicine and Animal Science Gazipur Agricultural University Gazipur Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-017-1190-0"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/1745039X.2019.1690389"},{"key":"e_1_2_8_4_1","first-page":"366","article-title":"The Impact of Silver Nanoparticles on Growth Performance, Lymphoid Organs and Oxidative Stress Indicators in Broiler Chicks","volume":"5","author":"Ahmadi F.","year":"2010","journal-title":"Global Veterinaria"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2024.104026"},{"key":"e_1_2_8_6_1","first-page":"139","volume-title":"Organic Feed Additives for Livestock","author":"Amr E.","year":"2025"},{"key":"e_1_2_8_7_1","first-page":"605","article-title":"Effects of Feed Type With\/Without Nanosil on Cumulative Performance, Relative Organ Weight and Some Blood Parameters of Broilers","volume":"7","author":"Andi M. 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