{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:14:00Z","timestamp":1772118840559,"version":"3.50.1"},"reference-count":44,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,7,24]],"date-time":"2025-07-24T00:00:00Z","timestamp":1753315200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100016003","name":"Istanbul \u00dcniversitesi-Cerrahpasa","doi-asserted-by":"publisher","award":["35655"],"award-info":[{"award-number":["35655"]}],"id":[{"id":"10.13039\/501100016003","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The increasing prevalence of antimicrobial resistance in animals, particularly the spread of multidrug\u2010resistant Enterobacterales, poses a significant zoonotic and public health risk.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The aim of this study was to investigate extended\u2010spectrum \u03b2\u2010lactamase (ESBL), carbapenem and colistin resistance among Enterobacterales in faecal swabs of dairy cattle.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A total of 400 samples were cultured on Mac Conkey screening media for ESBL, carbapenem and colistin resistance. The grown Enterobacterales were identified by MALDI\u2010TOF\u2010MS, followed by ceftriaxone, cefotaxime and ceftazidime resistance and double disk synergy. ESBL resistance genes were identified by polymerase chain reaction (PCR) and Sanger sequencing. Bacteria grown on colistin screening media were investigated for colistin resistance by EUCAST microbroth dilution method.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 89 (22.25%) of the bacteria grown from 400 samples were identified as potential ESBL\u2010producing Enterobacterales members. A number of 53 (59.5%) of them were identified as ESBL\n                      <jats:italic>blaCTX\u2010M<\/jats:italic>\n                      as a result of PCR, and 10 of them were identified as\n                      <jats:italic>blaCTX\u2010M\u201015\/28\/36\/66<\/jats:italic>\n                      as a result of sequencing. None of the samples cultured on carbapenem medium grew. A total of 18 samples grown in colistin medium were found to be colistin sensitive by broth microdilution. Genotypes were not included in the study. All isolated bacteria were identified as\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Solution<\/jats:title>\n                    <jats:p>\n                      In this study,\n                      <jats:italic>blaCTX\u2010M\u201015<\/jats:italic>\n                      and its derivatives, which are common in humans, were also found to be the predominant ESBL type in animals. Monitoring resistance in animals together with resistance in human infections may provide more important data on the spread of resistance.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70523","type":"journal-article","created":{"date-parts":[[2025,7,24]],"date-time":"2025-07-24T14:39:57Z","timestamp":1753367997000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Investigation of\n                    <i>CTX\u2010M<\/i>\n                    Type Extended\u2010Spectrum \u03b2\u2010Lactamase, Carbapenem and Colistin Resistance in Enterobacterales Isolated From Dairy Cattle in Turkey"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7451-4149","authenticated-orcid":false,"given":"Metin","family":"Yalcin","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology Cerrahpasa Faculty of Medicine Istanbul University\u2010Cerrahpasa  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sinem \u00d6zlem","family":"Enginler","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Istanbul University\u2010Cerrahpasa  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mert Ahmet","family":"Ku\u015fkucu","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Ko\u00e7 University  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0310-0966","authenticated-orcid":false,"given":"Mert","family":"Sar\u0131lar","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Istanbul University\u2010Cerrahpasa  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Selma","family":"Yal\u00e7\u0131n","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Cerrahpasa Faculty of Medicine Istanbul University\u2010Cerrahpasa  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00d6mer","family":"K\u00fc\u00e7\u00fckbasmac\u0131","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Cerrahpasa Faculty of Medicine Istanbul University\u2010Cerrahpasa  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,7,24]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/1304793"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2021.04.030"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.9775\/kvfd.2016.15832"},{"issue":"9","key":"e_1_2_11_5_1","first-page":"383","article-title":"One Health\u2014Attaining Optimal Health for People, Animals, and the Environment","volume":"5","author":"Atlas R.","year":"2010","journal-title":"Microbe"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.33988\/auvfd.828306"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.7754\/Clin.Lab.2019.180705"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.1.1\u20107.2004"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkn084"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2013.02.008"},{"issue":"2","key":"e_1_2_11_11_1","first-page":"1","article-title":"Geni\u015flemi\u015f Spektrumlu Beta\u2010Laktamazlar (GSBL) Ve Tan\u0131 Y\u00f6ntemleri","volume":"1","author":"Da\u011flar D.","year":"2012","journal-title":"Annals of Health Sciences Research"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010023\u201036240\u2010y"},{"key":"e_1_2_11_13_1","volume-title":"Mandell, Douglas and Bennett's Infectious Disease Essentials","author":"Donnenberg M. 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