{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T08:45:43Z","timestamp":1793263543780,"version":"4.5.0"},"reference-count":46,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T00:00:00Z","timestamp":1753660800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Mycobacterium bovis<\/jats:italic>\n                      , the cause of bovine tuberculosis (bTB), is a contagious, notifiable, chronic bacterial disease that causes economic losses. bTB remains a significant zoonotic threat, particularly in resource\u2010limited settings where reliable prevalence data are still lacking.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study aimed to determine the serological prevalence and molecular detection of bTB from different commercial and individual dairy farms of Sylhet and Sunamganj districts in Bangladesh.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A cross\u2010sectional study was conducted on a total of 250 blood and 250 milk samples, which were collected across selected dairy farms. Serological testing was performed using indirect ELISA, and molecular detection of\n                      <jats:italic>M. bovis<\/jats:italic>\n                      was carried out through PCR. Associations between infection status and potential risk factors were evaluated using chi\u2010square tests and logistic regression models.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The overall prevalence of\n                      <jats:italic>M. bovis<\/jats:italic>\n                      was 10.6%. Molecular detection through PCR revealed a higher prevalence in milk (14.8%) compared to blood (6.4%) samples. Serological testing showed that the prevalence in Sylhet district was lower (5.3%) than in Sunamganj district (12.0%). The highest prevalence was observed in diarrhoeic animals (19.49%), followed by female cattle (13.58%) and Holstein Friesian cows (14.0%). Cattle aged 1\u20133 years showed a notable prevalence of 17.51%.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The presence of\n                      <jats:italic>M. bovis<\/jats:italic>\n                      in dairy cattle herds was confirmed by both molecular and serological methods, highlighting the endemic nature of bTB in the study area. These findings underscore the importance of routine surveillance, combining diagnostic approaches and implementing effective control measures to reduce the burden of bTB in dairy production systems.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70531","type":"journal-article","created":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T14:14:37Z","timestamp":1753712077000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Sero\u2010Prevalence and Molecular Detection of\n                    <i>Mycobacterium bovis<\/i>\n                    in Cattle at Sylhet Division of Bangladesh"],"prefix":"10.1002","volume":"11","author":[{"given":"Md. Atik","family":"Faysal","sequence":"first","affiliation":[{"name":"Department of Medicine, Faculty of Veterinary Animal and Biomedical Sciences, Sylhet Agricultural University  Sylhet Bangladesh"},{"name":"School of Veterinary Medicine and Biomedical Sciences, University of Nebraska\u2010Lincoln  Lincoln Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Shahidur Rahman","family":"Chowdhury","sequence":"additional","affiliation":[{"name":"Department of Medicine, Faculty of Veterinary Animal and Biomedical Sciences, Sylhet Agricultural University  Sylhet Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatema Yeasmin","family":"Tanni","sequence":"additional","affiliation":[{"name":"Department of Medicine, Faculty of Veterinary Animal and Biomedical Sciences, Sylhet Agricultural University  Sylhet Bangladesh"},{"name":"Department of Biochemistry University of Nebraska\u2010Lincoln  Lincoln Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-4698-3128","authenticated-orcid":false,"given":"Hemayet","family":"Hossain","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology, Faculty of Veterinary Animal and Biomedical Sciences, Sylhet Agricultural University  Sylhet Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khadiza Akter","family":"Brishty","sequence":"additional","affiliation":[{"name":"Department of Zoology (GSSC) University of Dhaka  Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0045-9669","authenticated-orcid":false,"given":"Md. Bashir","family":"Uddin","sequence":"additional","affiliation":[{"name":"Department of Medicine, Faculty of Veterinary Animal and Biomedical Sciences, Sylhet Agricultural University  Sylhet Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2062-8471","authenticated-orcid":false,"given":"Md. Masudur","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Veterinary Animal and Biomedical Sciences, Sylhet Agricultural University  Sylhet Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Mahfujur","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Medicine, Faculty of Veterinary Animal and Biomedical Sciences, Sylhet Agricultural University  Sylhet Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8506-1202","authenticated-orcid":false,"given":"Md. Mukter","family":"Hossain","sequence":"additional","affiliation":[{"name":"Department of Medicine, Faculty of Veterinary Animal and Biomedical Sciences, Sylhet Agricultural University  Sylhet Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,28]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e34567"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3906\/VET\u20101811\u201062"},{"key":"e_1_2_10_4_1","doi-asserted-by":"crossref","unstructured":"Augusta M. F.Tsasse H. 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