{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T21:12:07Z","timestamp":1790025127738,"version":"4.0.1"},"reference-count":53,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T00:00:00Z","timestamp":1754870400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Feline vector\u2010borne diseases are caused by various pathogens transmitted by arthropods. Many of these infections have zoonotic importance, and cats can serve as sentinels for monitoring the health of both humans and pets. However, there is a limited research on the vector\u2010borne and zoonotic diseases carried by feline populations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study aimed to detect the prevalence of selected vector\u2010borne and zoonotic infections among stray cats in Istanbul, T\u00fcrkiye, by molecular and phylogenetic techniques.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      DNA extracted from blood samples of 316 stray cats was analysed using conventional PCR assays to identify various pathogens, targeting genes 16S rRNA for\n                      <jats:italic>Anaplasma\/Ehrlichia\/Bartonella<\/jats:italic>\n                      spp., 18S rRNA for\n                      <jats:italic>Hepatozoon<\/jats:italic>\n                      spp., LT1 for\n                      <jats:italic>Leishmania<\/jats:italic>\n                      spp. and 529\u00a0bp\u2014Repetitive element for\n                      <jats:italic>Toxoplasma gondii<\/jats:italic>\n                      . Phylogenetic reconstructions were conducted based on the results.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Anaplasma\/Ehrlichia\/Bartonella<\/jats:italic>\n                      spp.,\n                      <jats:italic>Hepatozoon<\/jats:italic>\n                      spp. and\n                      <jats:italic>T. gondii<\/jats:italic>\n                      prevalence were 1.8%, 3.4% and 0.3%, respectively. In addition, sequencing revealed the following prevalences:\n                      <jats:italic>Ehrlichia canis<\/jats:italic>\n                      (0.3%),\n                      <jats:italic>Hepatozoon felis<\/jats:italic>\n                      (1.5%),\n                      <jats:italic>Hepatozoon canis<\/jats:italic>\n                      (0.3%),\n                      <jats:italic>Bartonella henselae<\/jats:italic>\n                      (0.3%),\n                      <jats:italic>Bartonella clarridgeiae<\/jats:italic>\n                      (0.3%) and\n                      <jats:italic>T. gondii<\/jats:italic>\n                      (0.3%). No\n                      <jats:italic>Leishmania<\/jats:italic>\n                      spp. or\n                      <jats:italic>Anaplasma<\/jats:italic>\n                      spp. DNA was detected in any of the samples. The\n                      <jats:italic>E. canis<\/jats:italic>\n                      16S rRNA gene sequence obtained in the study showed 100% homology with\n                      <jats:italic>E. canis<\/jats:italic>\n                      from Venezuela (human), and the\n                      <jats:italic>H. felis<\/jats:italic>\n                      18S rRNA gene sequence demonstrated 99.45%\u2013100% similarity with\n                      <jats:italic>H. felis<\/jats:italic>\n                      from T\u00fcrkiye (\n                      <jats:italic>Haemaphysalis parva<\/jats:italic>\n                      ).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      This study is the first to report molecular and phylogenetic findings of\n                      <jats:italic>E. canis<\/jats:italic>\n                      and\n                      <jats:italic>H. canis<\/jats:italic>\n                      in cats from T\u00fcrkiye. Notably,\n                      <jats:italic>E. canis<\/jats:italic>\n                      ,\n                      <jats:italic>Bartonella<\/jats:italic>\n                      spp. and\n                      <jats:italic>T. gondii<\/jats:italic>\n                      all have zoonotic potential, highlighting the need for surveillance within the framework of a One Health approach.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70552","type":"journal-article","created":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T14:02:39Z","timestamp":1754920959000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Prevalence and Sequence Analysis of Vector\u2010Borne Zoonotic Diseases in Stray Cats in 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