{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T19:39:53Z","timestamp":1773085193712,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T00:00:00Z","timestamp":1754956800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Milk production and composition are affected by genetic and environmental factors. Among key genetic regulators, the <jats:italic>POU1F1<\/jats:italic> and <jats:italic>DGAT1<\/jats:italic> genes play significant roles. <jats:italic>POU1F1<\/jats:italic> affects pituitary gland functions and hormone secretion, indirectly impacting milk production. <jats:italic>DGAT1<\/jats:italic> is crucial for milk fat synthesis. Understanding genetic variations in these genes can enhance breeding strategies for improved milk yield and quality.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study investigates the relationship between <jats:italic>POU1F1<\/jats:italic> and <jats:italic>DGAT1<\/jats:italic> genes and milk composition in cross\u2010bred Hamdani sheep.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Blood and milk samples from 70 sheep were analysed for genetic markers using PCR\u2010RFLP technique. Statistical analysis assessed the relationship between genotypes and milk composition.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Results showed that <jats:italic>DGAT1<\/jats:italic> genotypes (CC and CT) were in Hardy\u2013Weinberg Equilibrium (HWE), whereas <jats:italic>POU1F1<\/jats:italic> genotypes were not. C and T allele frequencies were found 0.9 and 0.1 for DGAT1, respectively. On the other hand, the frequencies of C and T alleles were 0.52 and 0.48 for <jats:italic>POU1F1<\/jats:italic>, respectively. For <jats:italic>DGAT1<\/jats:italic> gene, CT genotypes carrying ewes had higher milk fat compared to CC genotypes (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). However, no differences were observed in milk solids\u2010not\u2010fat, protein and lactose content between genotypes (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a00.05). Effect sizes were detected as 0.83 (large) for fat percentage comparison, 0.09 (small) for percentage of solids\u2010not\u2010fat and 0.06 (small) for percentage of protein and lactose. Association analysis did not perform for <jats:italic>POU1F1<\/jats:italic> gene due to low sample size in CC genotype.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings suggest that <jats:italic>DGAT1<\/jats:italic> variations may influence milk fat content, whereas <jats:italic>POU1F1<\/jats:italic>\u2019s role remains unclear due to limited genotype variation. Further research is needed to clarify genetic influences of <jats:italic>DGAT1<\/jats:italic> and <jats:italic>POU1F1<\/jats:italic> genes on sheep milk in cross\u2010bred Hamdani sheep.<\/jats:p><\/jats:sec>","DOI":"10.1002\/vms3.70565","type":"journal-article","created":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T11:34:58Z","timestamp":1754998498000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Association of <i>DGAT1<\/i> and <i>POU1F1<\/i> Gene Polymorphisms With the Milk Traits in the Cross\u2010Bred Hamdani Sheep Bred Under Extensive Management"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6863-0939","authenticated-orcid":false,"given":"Ali Osman","family":"Turgut","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Medicine Department of Animal Science Siirt University  Siirt T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7962-6959","authenticated-orcid":false,"given":"Davut","family":"Koca","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Department of Obstetrics and Gynecology Van Y\u00fcz\u00fcnc\u00fc Yil University  Van T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9471-6410","authenticated-orcid":false,"given":"Mehmet","family":"Ero\u011flu","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Department of Animal Science Siirt University  Siirt T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,12]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-03206-y"},{"issue":"2","key":"e_1_2_11_3_1","first-page":"2082","article-title":"Relationship of POU1F1 Gene Polymorphism With Some of Economical Traits in Iraqi Awassi Ewes","volume":"6","author":"Al\u2010Khuzai H. M.","year":"2018","journal-title":"Journal of Entomology and Zoology Studies"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.4236\/eng.2012.410B008"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.12681\/jhvms.18879"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erq286"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10709-005-0034-6"},{"issue":"1","key":"e_1_2_11_8_1","first-page":"9","article-title":"DGAT1, CAST and IGF\u2010I Gene Polymorphisms in Akkaraman Lambs and Their Effects on Live Weights up to Weaning Age","volume":"25","author":"Bayram D.","year":"2019","journal-title":"Kafkas Universitesi Veteriner Fakultesi Dergisi"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M106219200"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.17221\/81\/2024-VETMED"},{"issue":"2","key":"e_1_2_11_11_1","first-page":"33","article-title":"Technical, Physical, Chemical and Microbiological Analyses of Siirt Herby Cheese","volume":"3","author":"G\u00fclmez M.","year":"2022","journal-title":"Bozok Veterinary Sciences"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470999738.ch7"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/vms3.1572"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/vms3.1450"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani10060997"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.36483\/vanvetj.1353378"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1415-47572007000600012"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/10495398.2013.763816"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-012-1525-z"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.56808\/2985-1130.2735"},{"issue":"4","key":"e_1_2_11_21_1","first-page":"331","article-title":"Polymorphism of Sheep POU1F1 Gene Exon 6 and 3'UTR Region and Their Association With Milk Production Traits","volume":"15","author":"Ozmen O.","year":"2014","journal-title":"Iranian Journal of Veterinary Research"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.15835\/buasvmcn-vm:9555"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/1746\u20106148\u20102\u201032"},{"issue":"2","key":"e_1_2_11_24_1","first-page":"271","article-title":"Milk Composition Traits of Hamdani Crossbreed Sheep Raised Under Extensive Management","volume":"7","author":"Turgut A. O.","year":"2023","journal-title":"ISPEC Journal of Agricultural Sciences"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.31797\/vetbio.1402523"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.3923\/ajava.2011.460.468"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10528-007-9100-3"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.3406\/paleo.1999.4684"}],"container-title":["Veterinary Medicine and Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.70565","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T12:02:10Z","timestamp":1758715330000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/vms3.70565"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,12]]},"references-count":27,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["10.1002\/vms3.70565"],"URL":"https:\/\/doi.org\/10.1002\/vms3.70565","archive":["Portico"],"relation":{},"ISSN":["2053-1095","2053-1095"],"issn-type":[{"value":"2053-1095","type":"print"},{"value":"2053-1095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,12]]},"assertion":[{"value":"2025-02-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70565"}}