{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T12:40:26Z","timestamp":1758717626404,"version":"3.44.0"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T00:00:00Z","timestamp":1754870400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Fibrosarcomas are most frequently encountered in senior dogs and cats, but they have also been documented in various other species such as alpacas, llamas, ferrets, cows, sheep, goats and horses. Macroscopic, microscopic and immunohistochemical features of a very rare and unusual case of fibrosarcoma with metastasis to several visceral organs in a 4 year\u2010old Holstein cow is reported for the first time.<\/jats:p><\/jats:sec><jats:sec><jats:title>Case summary<\/jats:title><jats:p>A cow with a history of decreased appetite, decreased milk production and chronic mastitis was presented to Abarkuh abattoir in Yazd province 4 months post\u2010parturition. The routine examination and systematic meat inspection revealed multi\u2010nodular focal masses, yellowish\u2010white in colour with firm consistency and clear borders on the surface and depth of the liver, kidney and heart, as well as in the parenchyma of both lungs. The masses showed a smooth surface without scars, bleeding and necrotic lesions. The tissue samples (1\u00a0\u00d7\u00a01\u00a0\u00d7\u00a01\u00a0cm<jats:sup>3<\/jats:sup>) were taken from the masses. Tissues were fixed in 10% buffered formalin and stained with haematoxylin and eosin, Masson's trichrome, and immunohistochemical staining using anti\u2010desmin, smooth muscle antibody (SMA), vimentin and CD34 antibodies. The histopathological evaluation of the masses showed spindle\u2010shaped cells, pleomorphic nuclei with mitotic figures and little cytoplasm. Masson's trichrome staining showed variable collagen fibres between abnormal fibroblasts. Immunohistochemical staining showed positive immunoreactivity for vimentin and negative for desmin, SMA and CD34 antibodies that are useful to distinguish this type of cancer from other spindle cell sarcomas. On the basis of the most recent literature search, this is the first report of multi\u2010organ metastasis of fibrosarcoma in a Holstein cow in the world.<\/jats:p><\/jats:sec>","DOI":"10.1002\/vms3.70567","type":"journal-article","created":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T13:56:34Z","timestamp":1754920594000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Histological and Immunohistochemical Findings of an Extremely Rare Multi\u2010Organ Metastasis of Fibrosarcoma in a Holstein Cow"],"prefix":"10.1002","volume":"11","author":[{"given":"Abdolrasoul","family":"Namjou","sequence":"first","affiliation":[{"name":"Department of Veterinary Shahrekord Branch Islamic Azad University Shahrekord Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reza","family":"Kheyrandish","sequence":"additional","affiliation":[{"name":"Department of Pathology School of Veterinary Medicine Kerman University  Kerman Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akbar","family":"GholamZadeh","sequence":"additional","affiliation":[{"name":"Department of Pathology School of Veterinary Medicine Kerman University  Kerman Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatemeh Zahra","family":"Kiani","sequence":"additional","affiliation":[{"name":"Department of Pathobiology School of Veterinary Medicine Shahrekord University  Shahrekord Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4137-9056","authenticated-orcid":false,"given":"Sadegh","family":"Shirian","sequence":"additional","affiliation":[{"name":"Department of Pathobiology School of Veterinary Medicine Shahrekord University  Shahrekord Iran"},{"name":"Shiraz Molecular Pathology Research Center Dr. Daneshbod Path Lab Shiraz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.20136"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e3181ef70b6"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1740\u20108261.1998.tb00320.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0929\u20106646(08)60189\u20108"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1055\/s\u20100041\u20101731462"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1354\/vp.39-1-33"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102482\u201013\u2010S2\u2010S42"},{"key":"e_1_2_9_10_1","first-page":"570","article-title":"A Case of Congenital Fibrosarcoma in a Calf","volume":"51","author":"Damodaran S.","year":"1974","journal-title":"Indian Veterinary Journal"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/1040638714529951"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470376928.ch2"},{"key":"e_1_2_9_13_1","first-page":"126","volume-title":"Text Book of Enzinger and Weiss's Soft Tissue Tumors","author":"Gown A. 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