{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T01:49:31Z","timestamp":1793324971137,"version":"4.5.0"},"reference-count":45,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T00:00:00Z","timestamp":1755648000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U2002218"],"award-info":[{"award-number":["U2002218"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81874274"],"award-info":[{"award-number":["81874274"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Scrub typhus, also known as Tsutsugamushi disease (TD), is an acute febrile illness that poses a significant public health threat to nearly one billion people in the Asia\u2010Pacific region. The disease is caused by\n                      <jats:italic>Orientia tsutsugamushi<\/jats:italic>\n                      (\n                      <jats:italic>OT<\/jats:italic>\n                      ) and is primarily transmitted through the bite of chigger mite larvae. Due to its nonspecific clinical manifestations, early diagnosis is challenging, often leading to misdiagnosis or untreated cases. Yunnan Province, located on China's border, reports the highest annual incidence of scrub typhus, highlighting the need to better understand\n                      <jats:italic>OT<\/jats:italic>\n                      infection in both human patients and small mammals in this region.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this study, clinical data on scrub typhus cases in Xiangyun County People's Hospital were retrospectively collected. Nested polymerase chain reaction (PCR) and semi\u2010nested PCR were used to amplify the 56\u2010kDa type\u2010specific antigen (TSA) from samples collected from 64 patients and 93 small mammals, representing six genera and nine species, in Xiangyun County, Yunnan Province, China. Complete sequences of the 56\u2010kDa TSA for each genotype were obtained through primer\u2010walking and subjected to evolutionary and recombination analyses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 377 scrub typhus cases were enrolled at Xiangyun County Hospital from July 2015 to November 2020. The detection rates of\n                      <jats:italic>OT<\/jats:italic>\n                      in patients and small mammals were 28.13% (18\/64) and 13.98% (13\/93), respectively. Nine full\u2010length 56\u2010kDa TSA genes were identified, comprising four distinct genotypes: Karp\u2010like (\n                      <jats:italic>n<\/jats:italic>\n                      = 3), Kato\u2010like (\n                      <jats:italic>n<\/jats:italic>\n                      = 3), Gilliam (\n                      <jats:italic>n<\/jats:italic>\n                      = 2), and TA763 (\n                      <jats:italic>n<\/jats:italic>\n                      = 1). Patients infected with the Karp\u2010like exhibited significantly more severe clinical symptoms, and the Karp\u2010like genotype of patients was relatively common in Xiangyun County of Yunnan Province. The Gilliam\u2010like and Karp\u2010like genotypes were detected in the infected small mammals, and\n                      <jats:italic>Apodemus chevrier<\/jats:italic>\n                      <jats:italic>i<\/jats:italic>\n                      is a dominant species in cultivated fields. Recombination analysis revealed genetic overlap among strains of similar or different genotypes, suggesting that genetic diversity may contribute to the increased incidence of scrub typhus.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      At least four genotypes of\n                      <jats:italic>OT<\/jats:italic>\n                      were present among patients, and it was infected by\n                      <jats:italic>OT<\/jats:italic>\n                      that at least four species of \u00a0infected small mammals in cultivated areas and at least\u00a0three species of infected small mammals in residential areas from Xiangyun County, Yunnan Province. The diversity of\n                      <jats:italic>OT<\/jats:italic>\n                      genotypes and infected small mammals, which causes frequent genetic recombination, is the main reason for the adaptive selection due to the genetic evolution of\n                      <jats:italic>OT<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70573","type":"journal-article","created":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T14:33:04Z","timestamp":1755700384000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Molecular Epidemiology and Genetic Diversity of\n                    <i>Orientia tsutsugamushi<\/i>\n                    From Patients and Small Mammals in Xiangyun County, Yunnan Province, China"],"prefix":"10.1002","volume":"11","author":[{"given":"Li","family":"Chen","sequence":"first","affiliation":[{"name":"Institute of Preventive Medicine School of Public Health Yunnan Key Laboratory of Screening and Research on Anti\u2010Pathogenic Plant Resources from Western Yunnan Yunnan University Key Laboratory of Zoonotic Disease Cross\u2010Border Prevention and Quarantine Dali University  Dali China"},{"name":"Department of Public health The People's Hospital of Leshan  Leshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Chen","family":"Kong","sequence":"additional","affiliation":[{"name":"Institute of Preventive Medicine School of Public Health Yunnan Key Laboratory of Screening and Research on Anti\u2010Pathogenic Plant Resources from Western Yunnan Yunnan University Key Laboratory of Zoonotic Disease Cross\u2010Border Prevention and Quarantine Dali University  Dali China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia\u2010Wei","family":"Tian","sequence":"additional","affiliation":[{"name":"Institute of Preventive Medicine School of Public Health Yunnan Key Laboratory of Screening and Research on Anti\u2010Pathogenic Plant Resources from Western Yunnan Yunnan University Key Laboratory of Zoonotic Disease Cross\u2010Border Prevention and Quarantine Dali University  Dali China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei\u2010Yu","family":"Han","sequence":"additional","affiliation":[{"name":"Institute of Preventive Medicine School of Public Health Yunnan Key Laboratory of Screening and Research on Anti\u2010Pathogenic Plant Resources from Western Yunnan Yunnan University Key Laboratory of Zoonotic Disease Cross\u2010Border Prevention and Quarantine Dali University  Dali China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Preventive Medicine School of Public Health Yunnan Key Laboratory of Screening and Research on Anti\u2010Pathogenic Plant Resources from Western Yunnan Yunnan University Key Laboratory of 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