{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T03:00:45Z","timestamp":1792810845978,"version":"4.5.0"},"reference-count":52,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T00:00:00Z","timestamp":1757289600000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2025,9]]},"abstract":"ABSTRACT<\/jats:title>\n \n \n \n The emergence of antimicrobial resistance\u00a0(AMR)\n Escherichia coli<\/jats:italic>\n in poultry farming is a growing global public health concern, particularly in Bangladesh, where the use of antibiotics remains largely unregulated. This study aimed to determine the prevalence and AMR patterns of\n E. coli<\/jats:italic>\n isolated from broiler chickens in Sylhet district of Bangladesh and to investigate the network of coexisting resistance traits among the isolates. A total of 130 samples (44 cloacal, 46 faecal, 21 liver and 19 intestinal) were collected from live and dead broiler chickens in the Sylhet district of Bangladesh from July 2020 to June 2021.\n E. coli<\/jats:italic>\n was detected in 77.7% of samples by standard cultural and biochemical tests, with 65.4% confirmed by polymerase chain reaction (PCR) targeting the\n malB<\/jats:italic>\n gene. Antibiotic susceptibility testing revealed complete (100%) resistance to tetracycline (TE), cloxacillin (CLOX) and co\u2010trimoxazole (COT), with 91.8% resistance to erythromycin (E). Gentamicin (GEN) exhibited intermediate resistance (69.4%), whereas azithromycin (AZM) was the most sensitive, with 58.8% of the isolates demonstrating susceptibility. Faecal samples had the highest\n E. coli<\/jats:italic>\n prevalence (84.8%), and liver samples had the lowest (66.7%). All isolates demonstrated multidrug resistance (MDR) in different combinations, with over one\u2010third exhibiting resistance to six or more antibiotics. The coexistence network revealed that resistance to TE, CLOX and COT frequently occurred together, whereas GEN exhibited a distinct resistance pattern characterized by limited co\u2010resistance with other antibiotics. The findings of this study extend beyond local concerns, carrying global implications for food safety, and emphasize the urgent need for stricter antibiotic regulations to mitigate the zoonotic transmission of MDR\n E. coli<\/jats:italic>\n to humans.\n <\/jats:p>\n <\/jats:sec>\n \n Summary<\/jats:title>\n \n \n \n \n The study reported a 77.7% prevalence of\n Escherichia coli<\/jats:italic>\n in broiler chickens in Sylhet, Bangladesh with alarming resistance patterns, including complete (100%) resistance to several antibiotics (tetracycline, cloxacillin and co\u2010trimoxazole), underscoring an urgent public health concern.\n <\/jats:p>\n <\/jats:list-item>\n \n The results revealed critical resistance trends, showing that several antibiotics are losing their effectiveness, which could threaten sustainable poultry farming and food safety.<\/jats:p>\n <\/jats:list-item>\n \n \n The correlation and coexistence network analysis identified frequent resistance linkages among specific antibiotics, suggesting shared pathways that could drive co\u2010selection in resistant\n E. coli<\/jats:italic>\n populations.\n <\/jats:p>\n <\/jats:list-item>\n \n \n The study emphasizes the pressing need for stricter antibiotic regulations, enhanced AMR surveillance and improved biosecurity measures to mitigate the spread of multidrug\u2010resistant\n E. coli<\/jats:italic>\n , with implications for both human and animal health.\n <\/jats:p>\n <\/jats:list-item>\n <\/jats:list>\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/vms3.70576","type":"journal-article","created":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T15:10:02Z","timestamp":1757344202000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Prevalence and Antimicrobial Resistance Patterns of\n Escherichia coli<\/i>\n Isolated From Broiler Chickens in Sylhet District of Bangladesh"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-9913-1594","authenticated-orcid":false,"given":"Manna","family":"Roy","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology Faculty of Veterinary Animal and Biomedical Sciences, Sylhet Agricultural University Sylhet Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3180-3706","authenticated-orcid":false,"given":"Obaidul","family":"Islam","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Chungbuk National University Cheongju Chungbuk South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-1711-2299","authenticated-orcid":false,"given":"Md Altafur","family":"Rahman","sequence":"additional","affiliation":[{"name":"Graduate School of Innovation and Practice for Smart Society Hiroshima University Hiroshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9945-506X","authenticated-orcid":false,"given":"Sharmin Sultana","family":"Misty","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hygiene Faculty of Veterinary Science Bangladesh Agricultural University Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-9413-1721","authenticated-orcid":false,"given":"Raju","family":"Kurmi","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Animal and Biomedical Sciences Sylhet Agricultural University Sylhet Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-5426-0251","authenticated-orcid":false,"given":"Md Ashraful","family":"Islam","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Laboratory Medicine College of Veterinary Medicine Chungbuk National University Cheongju Chungbuk South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5847-3239","authenticated-orcid":false,"given":"Ahsan","family":"Raquib","sequence":"additional","affiliation":[{"name":"Department of Health Management Atlantic Veterinary College University of Prince Edward Island Charlottetown Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2715-6510","authenticated-orcid":false,"given":"Jahid Hasan","family":"Tipu","sequence":"additional","affiliation":[{"name":"Department of Clinical Science Faculty of Medicine University of Bergen Bergen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Anwar","family":"Hossain","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology Faculty of Veterinary Animal and Biomedical Sciences, Sylhet Agricultural University Sylhet Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Siddiqul","family":"Islam","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology Faculty of Veterinary Animal and Biomedical Sciences, Sylhet Agricultural University Sylhet Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,8]]},"reference":[{"key":"e_1_2_12_2_1","volume-title":"Cowan and Steel's Manual for the Identification of Medical Bacteria","author":"Cowan S. 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