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This study presents a comprehensive comparative genomic and immunoinformatics analysis of a field strain (\n                    <jats:italic>B. abortus<\/jats:italic>\n                    S19, BAS19) isolated from an aborted cattle placenta 3 years post\u2010vaccination in Erzurum, Turkey. Whole\u2010genome sequencing was performed using Oxford Nanopore Technology, followed by genome assembly, functional annotation and comparative analyses against the reference strain (\n                    <jats:italic>B. abortus<\/jats:italic>\n                    S19, BAR19). Genomic variations, including 1153 single nucleotide polymorphisms (SNPs), 120 insertions and 2501 deletions, were identified. Annotation revealed 772 hypothetical proteins in BAS19 compared to 604 in BAR19, with distinct differences in virulence\u2010associated genes. Immunoinformatics analysis of 95 outer membrane proteins (OMPs) indicated significant antigenic variation, with 47 proteins exhibiting epitope loss and 11 displaying novel epitope gains. Beta\u2010barrel structure prediction demonstrated a reduction in structural stability, with nine OMPs losing beta\u2010barrel motifs, potentially influencing host\u2010pathogen interactions. These findings highlight key genomic adaptations in BAS19 that may influence its immunogenic properties and vaccine efficacy. The results contribute to a deeper understanding of\n                    <jats:italic>B. abortus<\/jats:italic>\n                    genomic diversity, providing insights for the rational design of improved vaccines and therapeutics tailored to regional epidemiological needs.\n                  <\/jats:p>","DOI":"10.1002\/vms3.70593","type":"journal-article","created":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T20:21:03Z","timestamp":1762201263000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genomic and Immunoinformatics Insights Into a Bovine\u2010Derived\n                    <i>Brucella abortus<\/i>\n                    S19 Field Strain: Adaptations Impacting Vaccine Efficacy"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8722-591X","authenticated-orcid":false,"given":"Ali","family":"Arslan","sequence":"first","affiliation":[{"name":"Department of Molecular Biology and Genetics Graduate School of Science &amp; Engineering Yildiz Technical University  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9422-3402","authenticated-orcid":false,"given":"Emre","family":"Aktas","sequence":"additional","affiliation":[{"name":"Faculty of Art and Science Molecular Biology and Genetics Y\u0131ld\u0131z Technical University  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0905-6783","authenticated-orcid":false,"given":"Osman Ugur","family":"Sezerman","sequence":"additional","affiliation":[{"name":"Department of Biostatistics and Bioinformatics Institute of Health Sciences Acibadem Mehmet Ali Aydinlar University  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6858-7045","authenticated-orcid":false,"given":"Tulin","family":"Ozbek","sequence":"additional","affiliation":[{"name":"Faculty of Art and Science Molecular Biology and Genetics Y\u0131ld\u0131z Technical University  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,3]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2016.00030"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1088\/1361\u20106528\/acd8b7"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010micro\u2010090110\u2010102905"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102164\u201010\u2010352"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/bf00290574"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/MRA.01436\u201020"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep08365"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.01538\u201019"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2024.03.003"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.00486\u201007"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab688"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.4103\/0974\u20102727.72149"},{"key":"e_1_2_12_14_1","volume-title":"Brucellosis in Humans and Animals","author":"Corbel M. 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