{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:06:58Z","timestamp":1790294818720,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,8,22]],"date-time":"2025-08-22T00:00:00Z","timestamp":1755820800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Feline panleukopenia<\/jats:italic>\n                      virus (FPV) is a highly contagious and often fatal disease affecting domestic and wild felines. Accurate diagnosis and understanding of circulating strains are essential for effective control.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study aimed to evaluate the diagnostic accuracy of a rapid immunochromatographic (IC) antigen test compared to PCR for FPV detection in clinically suspected pet cats in Bangladesh. It also aimed to investigate the genetic and evolutionary characteristics of circulating FPV strains.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Faecal or rectal swab samples from suspected cats were tested using both IC strip tests and PCR. Sensitivity and specificity of the IC test were analysed using PCR as the reference. Partial sequencing of the\n                      <jats:italic>VP2<\/jats:italic>\n                      gene was performed on four PCR\u2010positive samples for phylogenetic and mutational analysis. Structural modelling of VP2 proteins was conducted to predict conformational changes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The IC test detected FPV in 84% of cases, whereas PCR confirmed only 60%, indicating a 24% false\u2010positive rate. PCR showed higher diagnostic reliability. FPV prevalence was 92% among unvaccinated cats. Phylogenetic analysis of VP2 sequences revealed close genetic similarity with Chinese and Portuguese strains, suggesting possible cross\u2010border transmission. Mutations such as A756G, A896G, E299G and T236I were consistently observed. Structural modelling indicated minor conformational changes in VP2.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion and clinical significance<\/jats:title>\n                    <jats:p>PCR offers superior accuracy over IC testing for FPV diagnosis. Mutational changes may impact antigenicity and diagnostic performance. Improved diagnostic accuracy, molecular surveillance and updated vaccination strategies are essential to control FPV outbreaks in feline populations.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70594","type":"journal-article","created":{"date-parts":[[2025,8,22]],"date-time":"2025-08-22T15:03:46Z","timestamp":1755875026000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Comparative Detection and Genetic Characterization of Feline Panleukopenia Virus in Bangladesh"],"prefix":"10.1002","volume":"11","author":[{"given":"Nurejunnati","family":"Jeba","sequence":"first","affiliation":[{"name":"Department of Microbiology and Hygiene Bangladesh Agricultural University  Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roni","family":"Mia","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hygiene Bangladesh Agricultural University  Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Masum Billah","family":"Tarafder","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hygiene Bangladesh Agricultural University  Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anandha","family":"Mozumder","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hygiene Bangladesh Agricultural University  Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raduyan","family":"Farazi","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hygiene Bangladesh Agricultural University  Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. M. Nazmul","family":"Hasan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hygiene Bangladesh Agricultural University  Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Bayazid","family":"Bostami","sequence":"additional","affiliation":[{"name":"Teaching and Training Pet Hospital and Research Center Chattogram Veterinary and Animal Sciences University  Chattogram Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9660-4949","authenticated-orcid":false,"given":"A. K. M. Anisur","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Medicine Bangladesh Agricultural University  Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul","family":"Mannan","sequence":"additional","affiliation":[{"name":"Teaching and Training Pet Hospital and Research Center Chattogram Veterinary and Animal Sciences University  Chattogram Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6901-3377","authenticated-orcid":false,"given":"Sharmin","family":"Akter","sequence":"additional","affiliation":[{"name":"Department of Physiology Bangladesh Agricultural University  Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8036-0190","authenticated-orcid":false,"given":"Sukumar","family":"Saha","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hygiene Bangladesh Agricultural University  Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tofazzal","family":"Islam","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology and Genetic Engineering Gazipur Agricultural University  Gazipur Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elcio","family":"Leal","sequence":"additional","affiliation":[{"name":"Institute of Biological Sciences Federal University of Par\u00e1  Belem Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio Charlys","family":"da Costa","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical Faculdade de Medicina Universidade de S\u00e3o Paulo  S\u00e3o Paulo, State of S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1487-5444","authenticated-orcid":false,"given":"Md. 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