{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T23:09:26Z","timestamp":1790982566000,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T00:00:00Z","timestamp":1757721600000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003852","name":"Regione Campania","doi-asserted-by":"publisher","award":["prot. N. 213605 del 4 Maggio 2020"],"award-info":[{"award-number":["prot. N. 213605 del 4 Maggio 2020"]}],"id":[{"id":"10.13039\/501100003852","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The potential role of stray dogs as reservoirs and disseminators of zoonotic antimicrobial\u2010resistant pathogens to humans has long been underestimated. This study aimed to assess the occurrence of antimicrobial\u2010resistant\n                    <jats:italic>Staphylococcus<\/jats:italic>\n                    spp. strains in the ear and nasal swabs collected from stray dogs housed at the Veterinary Hospital of Local Health Authority ASL Napoli 1 Centro. Out of 306 skin samples analysed, 256 bacterial strains were isolated using selective and differential media and identified through MALDI\u2010TOF MS technology. The antimicrobial resistance profiles of the isolated strains were determined using the Kirby\u2013Bauer disc diffusion method on Mueller\u2013Hinton agar, testing susceptibility to 19 antimicrobials across 10 classes.\n                    <jats:italic>Staphylococcus<\/jats:italic>\n                    spp. was isolated in 46% of cases (119\/256), with\n                    <jats:italic>Staphylococcus pseudintermedius<\/jats:italic>\n                    being the most prevalent species (50%; 59\/119), followed by\n                    <jats:italic>Staphylococcus simulans<\/jats:italic>\n                    (17%; 20\/119) and\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    (14%; 17\/119). The evaluation of the antimicrobial resistance profiles revealed a great circulation of methicillin\u2010resistant strains in the stray dog population, as highlighted by the high levels of resistance recorded for penicillin (83.2%; 99\/119), cefoxitin (72.3%; 86\/119) and oxacillin (62.2%; 74\/119). Notably, 85% (101\/119) of the isolates were classified as multidrug\u2010resistant, being resistant to three or more antimicrobial classes. These findings suggest that stray dogs may serve as important reservoirs of multidrug\u2010resistant staphylococci, highlighting the need for ongoing surveillance and monitoring of antimicrobial resistance in these animals, especially in the context of the One Health approach that links human, animal and environmental health.\n                  <\/jats:p>","DOI":"10.1002\/vms3.70599","type":"journal-article","created":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T17:10:16Z","timestamp":1757783416000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Occurrence and Antimicrobial Resistance Profiles of\n                    <i>Staphylococcus<\/i>\n                    spp. Strains Isolated From Healthy Stray Dogs"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1789-7603","authenticated-orcid":false,"given":"Francesca Paola","family":"Nocera","sequence":"first","affiliation":[{"name":"Dipartimento di Medicina Veterinaria e Produzioni Animali Universit\u00e0 degli Studi di Napoli Federico II  Naples Campania Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annunziata","family":"Romano","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Veterinaria e Produzioni Animali Universit\u00e0 degli Studi di Napoli Federico II  Naples Campania Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sinem","family":"Arslan","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Veterinaria e Produzioni Animali Universit\u00e0 degli Studi di Napoli Federico II  Naples Campania Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rossana","family":"Schena","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Veterinaria e Produzioni Animali Universit\u00e0 degli Studi di Napoli Federico II  Naples Campania Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Pizzano","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Veterinaria e Produzioni Animali Universit\u00e0 degli Studi di Napoli Federico II  Naples Campania Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Cappiello","sequence":"additional","affiliation":[{"name":"Azienda Sanitaria Locale Napoli 1 Centro  Naples Campania Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina Di","family":"Palma","sequence":"additional","affiliation":[{"name":"Azienda Sanitaria Locale Napoli 3 Sud  Naples Campania Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Lamagna","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Veterinaria e Produzioni Animali Universit\u00e0 degli Studi di Napoli Federico II  Naples Campania Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Pompameo","sequence":"additional","affiliation":[{"name":"Azienda Sanitaria Locale Napoli 1 Centro  Naples Campania Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luisa","family":"De Martino","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Veterinaria e Produzioni Animali Universit\u00e0 degli Studi di Napoli Federico II  Naples Campania Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,13]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.14202\/vetworld.2023.536\u2010545"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11121758"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0156052"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009961"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2021.1878688"},{"key":"e_1_2_10_7_1","volume-title":"CLSI, VET01S; Performance Standards for Antimicrobial Disk and Dilution Susceptibility Test for Bacteria Isolate From Animals","author":"CLSI","year":"2020"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11050599"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10040677"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.2340\/00015555\u20103466"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2021.12.005"},{"key":"e_1_2_10_12_1","unstructured":"European Committee on Antimicrobial Susceptibility Testing (EUCAST).2021. \u201cBreakpoint Tables for Interpretation of MICs and Zone Diameters. 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