{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T20:42:33Z","timestamp":1784580153260,"version":"3.55.0"},"reference-count":54,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:00:00Z","timestamp":1761609600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:00:00Z","timestamp":1761609600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Neonatal diarrhoea is one of the most common diseases in lambs, causing economic losses and welfare issues. The endothelial glycocalyx (eGC) plays a crucial role in vascular integrity and inflammatory regulation. However, its involvement in neonatal lamb diarrhoea remains unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study aimed to evaluate eGC damage in neonatal lambs with diarrhoea by assessing biomarkers, including nitric oxide (NO), heparan sulphate (HS), angiopoietin\u20102 (Ang\u20102) and A disintegrin and metalloproteinase 15 (ADAM15).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A total of 68 neonatal lambs with diarrhoea (39 infected with\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      and 29 with\n                      <jats:italic>Cryptosporidium parvum<\/jats:italic>\n                      ) and 20 healthy controls were included. Diarrhoea aetiology was confirmed via faecal antigen tests and microscopic examination. Blood samples were collected for hemogram and biomarker analysis using sheep\u2010specific ELISA kits.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results and Conclusions<\/jats:title>\n                    <jats:p>\n                      Lambs with diarrhoea showed significantly higher total leukocyte (white blood cell [WBC]), granulocyte (GRA) and monocyte (MON) counts (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.01). Serum NO concentrations were significantly elevated in lambs with diarrhoea, whereas HS and Ang\u20102 concentrations were lower (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.01).\n                      <jats:italic>E. coli<\/jats:italic>\n                      \u2013infected lambs had significantly higher ADAM15 concentrations than those with\n                      <jats:italic>Cryptosporidium<\/jats:italic>\n                      infection (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.001). These findings suggest that NO may have a protective and\/or anti\u2010inflammatory role in neonatal lamb diarrhoea, potentially maintaining eGC integrity despite the inflammatory process.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70621","type":"journal-article","created":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T17:40:23Z","timestamp":1761673223000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of Endothelial Glycocalyx Damage in Neonatal Lambs With Diarrhoea: A Biomarker\u2010Based Approach"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4582-8536","authenticated-orcid":false,"given":"Murat Kaan","family":"Durgut","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Internal Medicine Selcuk University  Konya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8210-6735","authenticated-orcid":false,"given":"Mahmut","family":"Ok","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Internal Medicine Selcuk University  Konya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2928-5452","authenticated-orcid":false,"given":"Merve","family":"Ider","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Internal Medicine Selcuk University  Konya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bunyamin","family":"Tras","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology Selcuk University  Konya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amir","family":"Naseri","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Internal Medicine Selcuk University  Konya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3277-0336","authenticated-orcid":false,"given":"Tugba Melike","family":"Parlak","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology Selcuk University  Konya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdulrahman","family":"Alhallaq","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Internal Medicine Selcuk University  Konya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suleyman Serhat","family":"Iyigun","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Internal Medicine Selcuk University  Konya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,28]]},"reference":[{"issue":"1","key":"e_1_2_10_2_1","first-page":"10","article-title":"Clinical, Hematologic, Sero\u2010Biochemical and IgE Response in Lambs With Diarrhea Caused by Eimeria","volume":"8","author":"Abdel\u2010Saeed H.","year":"2019","journal-title":"International Journal of Veterinary Science"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.5398\/tasj.2025.48.2.132"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/anae.12661"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2019.09.021"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2007.08.021"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-90941-w"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000136519.84279.7a"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.livsci.2008.06.009"},{"key":"e_1_2_10_10_1","first-page":"1","volume-title":"Pathology of Domestic Animals","author":"Brown C. 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