{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T11:37:21Z","timestamp":1778067441169,"version":"3.51.4"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:00:00Z","timestamp":1761004800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:00:00Z","timestamp":1761004800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012453","name":"China Agricultural Research System","doi-asserted-by":"publisher","award":["CARS\u201040\u2010K08"],"award-info":[{"award-number":["CARS\u201040\u2010K08"]}],"id":[{"id":"10.13039\/501100012453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Non\u2010alcoholic fatty liver disease (NAFLD) is a growing concern in both human and animal health, with nutritional strategies playing a key role in its management. This study used a laying hen model to evaluate the effects of two high\u2010fat diets, one containing margarine (MAR) and the other natural milk cream (NC) on hepatic health, egg production and gut microbiota composition. Both diets were formulated with 8% fat but differed in fatty acid profiles: MAR was rich in lauric and stearic acids, whereas NC contained more palmitic and oleic acids. Compared to NC, MAR\u2010fed hens showed significantly higher total cholesterol, low\u2010density lipoprotein cholesterol (LDL\u2010cholesterol), liver fat, relative liver weight, abdominal fat and serum markers of liver damage (aspartate aminotransferase [AST], alkaline phosphatase [ALP], gamma\u2010glutamyl transferase [GGT] and adiponectin) (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.05). Microbiota analysis revealed that although Firmicutes and Bacteroidetes dominated in both groups, MAR\u2010fed hens had lower microbial diversity (Shannon index) and altered relative abundance of Verrucomicrobia, Peptostreptococcaceae and Turicibacter (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.05), indicating microbial dysbiosis. These findings demonstrate that the type of dietary fat\u2014independent of total fat content\u2014strongly influences liver function and gut microbial balance in poultry. The novelty of this study lies in showing how different fat sources, despite equal inclusion levels, can distinctly modulate the gut\u2013liver axis. This provides practical feed formulation insights and advances understanding of the gut\u2013liver axis in animal health.\n                  <\/jats:p>","DOI":"10.1002\/vms3.70665","type":"journal-article","created":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T18:21:43Z","timestamp":1761070903000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Dietary Fat Sources Affect Hepatic Health, Performance and Gut Microbiota Composition in Laying Hens as Model"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-7572-3863","authenticated-orcid":false,"given":"Humera","family":"Hamid","sequence":"first","affiliation":[{"name":"Jiangxi Province Key Laboratory of Animal Nutrition and Feed Jiangxi Agricultural University  Nanchang China"},{"name":"State Key Laboratory of Animal Nutrition &amp; Feeding College of Animal Science and Technology China Agricultural University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao Jun","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition &amp; Feeding College of Animal Science and Technology China Agricultural University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen Xiang","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition &amp; Feeding College of Animal Science and Technology China Agricultural University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shi Meng","family":"Huang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition &amp; Feeding College of Animal Science and Technology China Agricultural University  Beijing China"},{"name":"Feed Safety and Healthy Livestock Beijing Jingwa Agricultural Innovation Center  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li Hong","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition &amp; Feeding College of Animal Science and Technology China Agricultural University  Beijing China"},{"name":"Feed Safety and Healthy Livestock Beijing Jingwa Agricultural Innovation Center  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lan Jiao","family":"Xu","sequence":"additional","affiliation":[{"name":"Jiangxi Province Key Laboratory of Animal Nutrition and Feed Jiangxi Agricultural University  Nanchang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming Ren","family":"Qu","sequence":"additional","affiliation":[{"name":"Jiangxi Province Key Laboratory of Animal Nutrition and Feed Jiangxi Agricultural University  Nanchang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiu Gang","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition &amp; Feeding College of Animal Science and Technology China Agricultural University  Beijing China"},{"name":"Feed Safety and Healthy Livestock Beijing Jingwa Agricultural Innovation Center  Beijing 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