{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T17:00:46Z","timestamp":1785085246370,"version":"3.55.0"},"reference-count":55,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T00:00:00Z","timestamp":1768780800000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Lumpy skin disease (LSD) is a vector\u2010transmitted viral disease that affects cattle and water buffaloes. The LSD virus (LSDV) is a double\u2010stranded DNA virus and is a member of the Poxviridae family. The first documented cases of LSD were reported in Zambia in 1929, from which it spread to other countries in sub\u2010Saharan Africa, as well as to countries in Europe and Asia.<\/jats:p>\n                  <jats:p>\n                    The present study documents the first occurrence of LSD in the North African region. Different samples were collected from infected cattle in Algeria and Tunisia, and morbidity data were recorded. To confirm the occurrence of LSDV, polymerase chain reaction (PCR) and sequencing were performed on the basis of the p32 gene. The symptoms associated with LSD infection in both countries include lymph node enlargement, the presence of lumps in several parts of the body and the occurrence of high fever. The PCR detected the p32 gene of the LSDV in 77.9% (60\/77) of the collected samples, and cattle skin, nasal and buccal swabs were identified as the most virulent materials. Phylogenetic analysis employing the p32 gene sequence from two amplicons revealed 100% sequence identity to GenBank\u2010published LSDV sequences and grouped with amplicons predominantly isolated from Egypt (\n                    <jats:italic>MZ665552.1<\/jats:italic>\n                    ), Zimbabwe (\n                    <jats:italic>KX033500.1<\/jats:italic>\n                    ), various Asian countries and Russia. It is important to note that the phylogenetic analysis based on the p32 gene sequence is limited and poorly discriminative in comparison to whole genome sequencing, which is better at identifying the cluster to which a circulating virus belongs.\n                  <\/jats:p>\n                  <jats:p>The implementation of the Neethling strain vaccine in Algeria and Tunisia, in conjunction with the unfavourable climatic conditions conducive to vector multiplication, resulted in a substantial decline in clinical cases.<\/jats:p>","DOI":"10.1002\/vms3.70686","type":"journal-article","created":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T13:28:44Z","timestamp":1768829324000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["First Molecular Characterisation of the Lumpy Skin Disease Virus From the North African Region, Algeria and Tunisia"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9270-9161","authenticated-orcid":false,"given":"Ilhem","family":"Zouyed","sequence":"first","affiliation":[{"name":"Institute of Veterinary Sciences University Fr\u00e8res Mentouri Constantine 1  Constantine Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hichem","family":"Soualah","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Sciences University Fr\u00e8res Mentouri Constantine 1  Constantine Algeria"},{"name":"Laboratoire de Recherche de Pathologie Animale D\u00e9veloppement des \u00c9levages et Surveillance de la chaine alimentaire des Denr\u00e9es Animales ou d'origine Animale (PADESCA Laboratory) Institute of Veterinary Sciences\u2010El Khroub, University of Constantine  Constantine Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samir","family":"Djemai","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Sciences University Fr\u00e8res Mentouri Constantine 1  Constantine Algeria"},{"name":"Laboratoire de Recherche de Pathologie Animale D\u00e9veloppement des \u00c9levages et Surveillance de la chaine alimentaire des Denr\u00e9es Animales ou d'origine Animale (PADESCA Laboratory) Institute of Veterinary Sciences\u2010El Khroub, University of Constantine  Constantine Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Messai","sequence":"additional","affiliation":[{"name":"Department of Agricultural Sciences, PIARA Research Laboratory University of Biskra  Biskra Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed El Hadi","family":"Daia","sequence":"additional","affiliation":[{"name":"Department of Agricultural Sciences, DEDSPAZA Research Laboratory University of Biskra  Biskra 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