{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T22:17:39Z","timestamp":1794003459428,"version":"4.8.0"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T00:00:00Z","timestamp":1763424000000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004763","name":"Natural Science Foundation of Inner Mongolia Autonomous Region","doi-asserted-by":"publisher","award":["2023MS08061"],"award-info":[{"award-number":["2023MS08061"]}],"id":[{"id":"10.13039\/501100004763","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To better understand the molecular biological characteristics and strain types of Brucella in Hulunbuir, Arun Banner, Inner Mongolia Autonomous Region, through isolation, biochemical identification, AMOS\u2010PCR, and multiple locus sequence typing (MLST) of Brucella strains from human brucellosis patients, and to provide a reference for the scientific prevention and control of brucellosis in this region.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>\n                      The clinical and epidemiological data of patients with human brucellosis in Arun Banner, Inner Mongolia, were collected and analysed. Aseptically collected samples included venous blood, midstream urine and fresh diseased tissues (cerebrospinal fluid, synovial fluid, bone marrow, lymph node aspirates and abscess puncture fluids). Gram staining, serum agglutination, dye inhibition and phage lysis tests were used for the identification of\n                      <jats:italic>Brucella<\/jats:italic>\n                      species and biovars. Molecular identification and typing of\n                      <jats:italic>Brucella<\/jats:italic>\n                      were performed using AMOS polymerase chain reaction and multiple locus sequence typing (MLST).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Nine Brucella strains were isolated, all being\n                      <jats:italic>B. melitensis<\/jats:italic>\n                      : 2 biovar 1, 4 biovar 2, 2 biovar 3, and 1 variant. All infected individuals (9\/9) were herdspersons, with a male\u2010to\u2010female ratio of 7:2 and an onset age of 30\u201363 years (mean 44). Among the cases, 88.89% became ill following contact with sheep, 66.67% were engaged in breeding work and 33.33% had direct contact with cattle and sheep. In MLST typing, seven strains were sequence type (ST) 8, one was a newly identified allele and one was ST12. Phylogenetic analysis using the neighbour\u2010joining method showed that all nine isolates clustered with\n                      <jats:italic>B. melitensis<\/jats:italic>\n                      reference strains (M5, M28, 16\u00a0M, Rev.1), consistent with biochemical and PCR identification results.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      People involved in raising and trafficking sheep constitute a high\u2010risk group for brucellosis. The strains isolated were\n                      <jats:italic>B. melitensis<\/jats:italic>\n                      , with ST8 as the predominant type identified by MLST typing. Attention should also be given to the discovery of new alleles.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70694","type":"journal-article","created":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T12:03:31Z","timestamp":1763467411000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Isolation, Identification and Typing of Nine Strains of\n                    <i>Brucella<\/i>\n                    in Arun Banner"],"prefix":"10.1002","volume":"11","author":[{"given":"Na","family":"Ta","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine Inner Mongolia Agricultural University  Hohhot China"},{"name":"Inner Mongolia Center for Disease Control and Research  Hohhot China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Gao","sequence":"additional","affiliation":[{"name":"School of Public Health Baotou Medical College  Baotou City China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke\u2010Ming","family":"Zuo","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine Inner Mongolia Agricultural University  Hohhot China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang\u2010Li","family":"Niu","sequence":"additional","affiliation":[{"name":"Hailar City Center for Disease Prevention and Control  Hailar China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong\u2010Ming","family":"Lin","sequence":"additional","affiliation":[{"name":"Arong Banner Center for Disease Prevention and Control  Hailar China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3514-1068","authenticated-orcid":false,"given":"Yong\u2010Jun","family":"Wen","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine Inner Mongolia Agricultural University  Hohhot China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai","family":"Jiang","sequence":"additional","affiliation":[{"name":"Chinese Center for Disease Control and Prevention  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,18]]},"reference":[{"issue":"17","key":"e_1_2_11_2_1","first-page":"14","article-title":"Prevention and Control of Brucellosis in Inner Mongolia","volume":"303","year":"2018","journal-title":"Veterinary Journal"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201096774\u2010x"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/2186027"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005792\u2010199607000\u201000003"},{"key":"e_1_2_11_6_1","volume-title":"Brucellosis in Humans and Animals","author":"Corbel M. 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