{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T11:14:39Z","timestamp":1788606879648,"version":"build-2803163510"},"reference-count":54,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T00:00:00Z","timestamp":1765411200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T00:00:00Z","timestamp":1765411200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100011665","name":"Deanship of Scientific Research, King Saud University","doi-asserted-by":"publisher","award":["ORF\u20102025\u20101393"],"award-info":[{"award-number":["ORF\u20102025\u20101393"]}],"id":[{"id":"10.13039\/501100011665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This experiment evaluated the effect of feeding cottonseed meal (CSM) and eight supplemental endo\u20101,4\u2010\u03b2\u2010xylanase on growth performance, carcass traits, blood parameters, nutrients nine digestibility, expression of insulin\u2010like growth factor (IGF\u20101) gene, intestinal health and economic efficiency in broiler chickens. Note that 601 days old male broiler chicks (Ross\u2010308) were randomly distributed into six groups. Each group divided into 10 replicates with 10 birds per replicate. Group A (control) was fed the basal diet (BD), group B received BD supplemented with endo\u20101,4\u2010\u03b2\u2010xylanase at 100\u00a0g\/ton feed and groups C and E fed a diet containing 50 and 100\u00a0kg\/ton CSM, respectively. Finally, the groups D and F fed a diet containing 50 and 100\u00a0kg\/ton CSM with 100\u00a0g\/ton enzyme, respectively. Feeding cottonseed meal with enzyme supplementation resulted in better final body weight, weight gain and feed conversion ratio (p &lt;0.05). Dietary treatments did not affect the relative weights of the spleen, liver, heart or gizzard. Feeding birds on CSM decreased the crude protein (CP) and carcass yield; however, they can be improved by xylanase supplementation (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.05). Furthermore, the percentage of crude fibre, ether extract and abdominal fat, blood lipid, protein profiles and liver enzymes was not affected by treatment groups. Moreover, the expression level of IGF\u20101 was significantly improved (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.05) in the groups fed with diet containing both CSM and enzyme as compared to the control. The length of intestinal villi reduced by CSM feeding and enhanced with addition of enzyme (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.05). The lowest feed cost per bird was observed for the diet containing 100\u00a0kg CSM. The highest net profit, benefit\u2010cost ratio and economic efficiency were recorded in the groups received diets having CSM at either dose with enzyme and that have 100\u00a0kg CSM without enzyme. In conclusion, the combination of CSM and xylanase could be a cost\u2010effective ingredient in the diets of broilers, substituting partially soybean meal.\n                  <\/jats:p>","DOI":"10.1002\/vms3.70723","type":"journal-article","created":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T07:10:11Z","timestamp":1765523411000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effect of Feeding Cottonseed Meal With or Without NSP Enzymes on Growth Performance, Blood Biochemistry, Growth\u2010Related Gene Expression, Intestinal Health and Economic Efficiency in Broilers"],"prefix":"10.1002","volume":"12","author":[{"given":"Ahmed A.","family":"Saleh","sequence":"first","affiliation":[{"name":"Department of Poultry Production Faculty of Agriculture Kafrelsheikh University  Kafrelsheikh Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibrahim A.","family":"Elkhaiat","sequence":"additional","affiliation":[{"name":"Department of Poultry Production Faculty of Agriculture Kafrelsheikh University  Kafrelsheikh Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Mansour","sequence":"additional","affiliation":[{"name":"Department of Poultry Production Faculty of Agriculture Kafrelsheikh University  Kafrelsheikh Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salwa","family":"Genedy","sequence":"additional","affiliation":[{"name":"Department of Poultry Production Faculty of Agriculture Kafrelsheikh University  Kafrelsheikh Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rashed A.","family":"Alhotan","sequence":"additional","affiliation":[{"name":"Department of Animal Production College of Food &amp; Agriculture Sciences King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elsayed Osman Sewlim","family":"Hussein","sequence":"additional","affiliation":[{"name":"AlKhumasia For Feed and Animal Products  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Branislav","family":"Galik","sequence":"additional","affiliation":[{"name":"Institute of Nutrition and Genomics Slovak University of Agriculture in Nitra  Nitra Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hana","family":"Hakami","sequence":"additional","affiliation":[{"name":"Department of Zoology College of Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed S.","family":"Sami","sequence":"additional","affiliation":[{"name":"Department of Zoology College of Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed A.","family":"Kamal","sequence":"additional","affiliation":[{"name":"Department of Veterinary Hygiene and Management Faculty of Veterinary Medicine Cairo University  Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nahed A.","family":"El\u2010Shall","sequence":"additional","affiliation":[{"name":"Department of Poultry and Fish Diseases Faculty of Veterinary Medicine Alexandria University  Alexandria Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3787-8805","authenticated-orcid":false,"given":"Samia","family":"Fawzy","sequence":"additional","affiliation":[{"name":"Department of Animal Wealth Development Faculty of Veterinary Medicine Kafrelsheikh University  Kafrelsheikh Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,11]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.5187\/jast.2020.62.2.159"},{"key":"e_1_2_11_3_1","volume-title":"Official Methods of Analysis of AOAC","author":"AOAC","year":"2003"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani14192797"},{"key":"e_1_2_11_5_1","volume-title":"Ross Broiler Management Handbook","author":"Aviagen","year":"2014"},{"key":"e_1_2_11_6_1","first-page":"104","article-title":"The Growth Performance of Broiler Chicks Fed With Diets Containing Cottonseed Meal Supplemented With Lysine","volume":"156","author":"Azman M.","year":"2005","journal-title":"Revue De M\u00e9decine V\u00e9t\u00e9rinaire"},{"key":"e_1_2_11_7_1","volume-title":"Theory and Practice of Histological Techniques","author":"Bancroft J. 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