{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T17:00:48Z","timestamp":1785085248447,"version":"3.55.0"},"reference-count":72,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T00:00:00Z","timestamp":1767139200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T00:00:00Z","timestamp":1767139200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Newcastle disease (ND) is a commonly fatal viral disease that has been reported to affect a wide range of avian hosts, irrespective of their age and sex. It is reported to be a major constraint to the development, survival, and productivity of poultry in Ethiopia. Despite its significant effect, limited studies were conducted regarding the current situation of ND in different parts of Ethiopia, particularly in the Western Oromia region. Thus, an outbreak\u2010based study was carried out from November 2020 to June 2021 in selected areas of northern and western Oromia with the aim of virus isolation and molecular detection from Newcastle disease suspected outbreak cases in chickens. A total of 98 samples (77 swabs and 21 pooled postmortem tissues) were purposively collected from clinically suspected chickens and examined for the presence of NDV by a one\u2010step real\u2010time polymerase chain reaction (real\u2010time RT\u2010PCR) test targeting a conserved region of the Matrix gene. From the clinical samples tested based on the one\u2010step real\u2010time RT\u2010PCR, 25 (25\/98) samples were positive for the NDV. From these, 22 of the positive samples were obtained from swabs, and the other 3 positive samples were from pooled postmortem tissue samples. Furthermore, among the 22 positive swab samples, 14 of the positive samples were of tracheal origin. ND\u2010positive samples were further subjected to cell culture for isolation of the NDV. All 25 real\u2010time RT\u2010PCR\u2010positive samples were inoculated into DF\u20101 cells for virus isolation, of which 18 of them were able to show CPE on the cell line. The other seven samples remained negative up to the third passage. Regular vaccination with further genetic analysis of the virus should be carried out to understand the specific genotype of the virus.<\/jats:p>","DOI":"10.1002\/vms3.70729","type":"journal-article","created":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T11:17:27Z","timestamp":1767179847000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Isolation and Molecular Detection of Newcastle Disease Virus From Outbreak Cases in Selected Areas of Northern and Western Oromia, Ethiopia"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5698-4385","authenticated-orcid":false,"given":"Yobsan","family":"Tamiru","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine Wollega University  Nekemte Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abde","family":"Aliyi","sequence":"additional","affiliation":[{"name":"Animal Health Institute  Sebeta Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Demesa","family":"Negessu","sequence":"additional","affiliation":[{"name":"Animal Health Institute  Sebeta Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,31]]},"reference":[{"key":"e_1_2_11_2_1","first-page":"39","article-title":"Seroprevalence and Associated Risk Factors of Infectious Bursal Disease in Chickens Managed Under Intensive and Backyard Production Systems in Western Oromia, Ethiopia","volume":"13","author":"Abdeta D.","year":"2022","journal-title":"Veterinary Medicine"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-019-01843-z"},{"issue":"81","key":"e_1_2_11_4_1","first-page":"18816","article-title":"Optimization of NDV Production in T\u2010Flask","volume":"10","author":"Arifin A.","year":"2011","journal-title":"African Journal of Biotechnology"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-020-02465-6"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2021.01.027"},{"key":"e_1_2_11_7_1","first-page":"234","article-title":"Presence of Newcastle Disease in Vaccinated Indigenous Chicken From Farms in Selected Regions in Kenya\u2010 a Cross Sectional Study","volume":"15","author":"Auleria A.","year":"2020","journal-title":"Journal of Virology"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874357901812010052"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.3329\/bvet.v27i1.5908"},{"issue":"1","key":"e_1_2_11_10_1","first-page":"05","article-title":"Isolation and Identification of Newcastle Disease Virus From Outbreak Cases and Apparently Healthy Local Chickens in South West Shewa, Ethiopia","volume":"6","author":"Belayheh G.","year":"2015","journal-title":"International Journal of Microbiological Research"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/7278459"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.5897\/AJB2017.15899"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e14384"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.5958\/0973-970X.2017.00037.2"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2011-01906"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-012-0278-y"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02716-13"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/2193-1801-3-459"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40064-016-2114-8"},{"key":"e_1_2_11_20_1","first-page":"5","article-title":"Occurrence of Newcastle Disease and Infectious Bursal Disease Virus Antibodies in Double\u2010Spurred Francolins in Nigeria","volume":"2014","author":"Daniel O.","year":"2014","journal-title":"Journal of Veterinary Medicine"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetvac.2022.100003"},{"key":"e_1_2_11_22_1","first-page":"6","article-title":"Serological and Molecular Study of NDV in Village Chickens in Selected Rift\u2010Valley Areas, Ethiopia","volume":"6","author":"Dehinenet T.","year":"2015","journal-title":"Veterinary Science and Technology"},{"key":"e_1_2_11_23_1","unstructured":"Desalegn J.2015. \u201cEpidemiology of Village Chicken Diseases: A Longitudinal Study on the Magnitude and Determinants of Morbidity and Mortality\u2014The Case of Newcastle and Infectious Bursal Disease.\u201d Phd diss. Addis Ababa University."},{"key":"e_1_2_11_24_1","unstructured":"Dessalew T. andT.Dehinet.2025. \u201cClinicopathological and Laboratory Diagnosis of Newcastle Disease.\u201d preprint Research Square April 9 2025.https:\/\/doi.org\/10.21203\/rs.3.rs\u20106391479\/v1."},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2019.103917"},{"issue":"7","key":"e_1_2_11_26_1","first-page":"1079","article-title":"Function Comparison of the Matrix Protein Between NDV and Other Paramyxoviruses","volume":"56","author":"Duan Z.","year":"2016","journal-title":"Virology"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.2147\/VMRR.S445261"},{"key":"e_1_2_11_28_1","first-page":"4","article-title":"Sero Prevalence of Newcastle Disease in Scavenging Chicken in Selected District of Aris Zone","volume":"3","author":"Endale M.","year":"2017","journal-title":"International Journal of Agriculture and Veterinary Science"},{"key":"e_1_2_11_29_1","unstructured":"Esubalew E.2021. \u201cMolecular Characterization for Strains of Newcastle Disease Virus Circulating in Chickens in Selected Migratory BirdsAbundant Areas in the Mid Rift Valley and Central Part of Ethiopia.\u201d PhD diss. Adama Science and Technology University."},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470649367"},{"key":"e_1_2_11_31_1","unstructured":"Gelana H.2017. \u201cConventional and Molecular Epidemiology of New Castle Disease in Wild and Domestic Birds in Selected Rift Valley Areas Ethiopia.\u201d Master's Thesis Addis Ababa University."},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.14202\/vetworld.2024.2688-2693"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.4314\/sokjvs.v14i3.3"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11262-009-0424-4"},{"issue":"2","key":"e_1_2_11_35_1","first-page":"87","article-title":"Isolation and Detection of NDV From Field Outbreaks in Broiler and Layer Chickens by Reverse Transcription\u2010Polymerase Chain Reaction","volume":"8","author":"Haque M.","year":"2010","journal-title":"Journal of Veterinary Medicine"},{"key":"e_1_2_11_36_1","first-page":"11","article-title":"Molecular Characterization of NDV From Backyard Poultry Farms and Live Bird Markets in Kenya","volume":"2018","author":"Irene N.","year":"2018","journal-title":"Hindawi International Journal of Microbiology"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2013.09.025"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.30466\/vrf.2022.548152.3373"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063870802000402"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms12102029"},{"key":"e_1_2_11_41_1","first-page":"63","article-title":"Detection of NDV Minor Genetic Variants by Modified Single\u2010Stranded Conformational Polymorphism Analysis","volume":"8","author":"Lukasz R.","year":"2014","journal-title":"Biomedical Research International"},{"key":"e_1_2_11_42_1","first-page":"253","article-title":"Serological Investigation of Newcastle Disease in Selected Districts of Buno Bedelle Zone, Ethiopia","volume":"12","author":"Mamo T.","year":"2021","journal-title":"Veterinary Medicine"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.3329\/bvet.v29i2.14341"},{"key":"e_1_2_11_44_1","first-page":"7","article-title":"Plaque Formation of LaSota Pathogenic Strain of NDV Adapted in Chick Embryo Fibroblast Cells","volume":"62","author":"Mehrabanpour M.","year":"2010","journal-title":"Archives of Razi Institute"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2013.06.007"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2009.05.033"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2013.06.007"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006403017578"},{"key":"e_1_2_11_49_1","first-page":"17","article-title":"Avian Paramyxovirus Serotype\u20101: A Review of Disease Distribution, Clinical Symptoms, and Laboratory Diagnostics","volume":"2012","author":"Nichole L.","year":"2012","journal-title":"Veterinary Medicine International"},{"key":"e_1_2_11_50_1","first-page":"555","volume-title":"Manual of Diagnostic Tests and Vaccines for Terrestrial Animals: (Mammals, Birds and Bees)","author":"OIE","year":"2017"},{"key":"e_1_2_11_51_1","volume-title":"Terrestrial Manual: Chapter 3.3.14","author":"OIE","year":"2018"},{"key":"e_1_2_11_52_1","first-page":"157","article-title":"Epidemiology and Characterization of Newcastle Disease in Smallholder Poultry in Mozambique","volume":"70","author":"Perttula L.","year":"2009","journal-title":"Journal of Veterinary Research"},{"key":"e_1_2_11_53_1","volume-title":"R Foundation for Statistical Computing","author":"R Core Team","year":"2017"},{"issue":"1","key":"e_1_2_11_54_1","first-page":"7","article-title":"Molecular Characterization of NDV Isolates From Different Geographical Regions in Mozambique","volume":"79","author":"Raul F.","year":"2012","journal-title":"Journal of Veterinary Research"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2008.12.008"},{"issue":"3","key":"e_1_2_11_56_1","first-page":"232","article-title":"Molecular Characterization of NDV (Genotype VII) From Broiler Chickens in Egypt","volume":"6","author":"Samar S.","year":"2017","journal-title":"Journal of Basic Applied Science"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pey559"},{"issue":"1","key":"e_1_2_11_58_1","first-page":"25","article-title":"Seroprevalence Study of Newcastle Disease in Local Chickens in Central Ethiopia","volume":"3","author":"Serkalem T.","year":"2005","journal-title":"International Journal of Applied Research of Veterinary Medicine"},{"issue":"1","key":"e_1_2_11_59_1","first-page":"73","article-title":"Isolation, Identification and Daptation of NDV Field Isolates in the Embryonated Chicken Eggs and Chicken Embryo Fibroblast Cells","volume":"2","author":"Sharmin J.","year":"2013","journal-title":"Journal of Biological Science"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.3923\/ijps.2005.472.475"},{"key":"e_1_2_11_61_1","unstructured":"Songolo A. andJ. C.Katongo.2000.Country Report: Zambia. SADC Planning Workshop on Newcastle Disease Control in Village Chickens. Proceedings. International Workshop Maputo Mozambique.ACIAR Proceedings. Pp.43\u201345."},{"key":"e_1_2_11_62_1","volume-title":"ILRI Manual Nairobi","author":"Tadiose H.","year":"2017"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/8915400"},{"issue":"4","key":"e_1_2_11_64_1","first-page":"1189","article-title":"Prevalence of Newcastle Disease in Chickens in Ethiopia: A Systematic Review and Meta\u2010analysis. World's","volume":"80","author":"Tibebu A.","year":"2024","journal-title":"Poultry Science Journal"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-020-02503-3"},{"key":"e_1_2_11_66_1","first-page":"555","volume-title":"Manual of Diagnostic Tests and Vaccines for Terrestrial Animals: (Mammals, Birds and Bees)","author":"WOAH","year":"2017"},{"key":"e_1_2_11_67_1","volume-title":"Terresterial Manual.2021. CHAPTER 3 .3.14","author":"WOAH","year":"2021"},{"key":"e_1_2_11_68_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.1.329-338.2004"},{"key":"e_1_2_11_69_1","first-page":"65","article-title":"Isolation and Molecular Detection of Newcastle Disease Virus From Field Outbreaks in Chickens in Central Ethiopia","volume":"13","author":"Worku T.","year":"2022","journal-title":"Veterinary Medicine"},{"key":"e_1_2_11_70_1","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-10-164"},{"key":"e_1_2_11_71_1","doi-asserted-by":"publisher","DOI":"10.34172\/ijmpes.4187"},{"key":"e_1_2_11_72_1","first-page":"384","article-title":"Investigation of Newcastle Disease Virus Using Reverse Transcription Polymerase Chain Reaction in Selected Districts of Eastern Shewa, Ethiopia","volume":"7","author":"Yemsrach M.","year":"2016","journal-title":"Journal of Veterinary Science and Technologyl"},{"key":"e_1_2_11_73_1","doi-asserted-by":"publisher","DOI":"10.1080\/03079450120078626"}],"container-title":["Veterinary Medicine and Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.70729","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/vms3.70729","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.70729","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T16:44:18Z","timestamp":1785084258000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/vms3.70729"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,31]]},"references-count":72,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1002\/vms3.70729"],"URL":"https:\/\/doi.org\/10.1002\/vms3.70729","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/VMS3.70729\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70729\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70729\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70729\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70729\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70729\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70729\/v1\/decision1","asserted-by":"object"}]},"ISSN":["2053-1095","2053-1095"],"issn-type":[{"value":"2053-1095","type":"print"},{"value":"2053-1095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,31]]},"assertion":[{"value":"2025-02-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70729"}}