{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T06:27:37Z","timestamp":1791872857986,"version":"4.3.4"},"reference-count":105,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T00:00:00Z","timestamp":1773014400000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Bovine bacterial mastitis is an inflammation of cows\u2019 udders by bacterial infection, leading to significant economic, animal welfare and public health consequences. Conventional diagnostic tests are expensive, time\u2010consuming, and require specialised laboratories, making them unsuitable for on\u2010farm surveillance and timely interventions. As an alternative, loop\u2010mediated isothermal amplification (LAMP) has gained attention as a rapid, cost\u2010effective and sensitive molecular diagnostic. LAMP amplifies DNA at a constant temperature, offering a simple and efficient method for bacterial detection. This review compiles studies on LAMP assays developed to detect major mastitis\u2010causing bacteria. Thus, a total of 18 LAMP assays were identified using PRISMA guidelines. Colourimetric and turbidity\u2010based LAMP assays were most frequently used.\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    ,\n                    <jats:italic>Streptococcus agalactiae<\/jats:italic>\n                    ,\n                    <jats:italic>Streptococcus uberis<\/jats:italic>\n                    and\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    were the most common bacteria detected. While LAMP demonstrates high sensitivity and specificity in laboratory settings, its efficacy in field conditions remains uncharacterised. Nevertheless, the simplicity of sample preparation, resistance to inhibitors, short turnaround time, ease of running and simple\u2010to\u2010interpret results make LAMP a promising method for on\u2010farm diagnosis. Overall, this review highlights the current state of LAMP developed for bovine mastitis and identifies gaps for future studies.\n                  <\/jats:p>","DOI":"10.1002\/vms3.70794","type":"journal-article","created":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T14:41:39Z","timestamp":1773067299000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Loop\u2010Mediated Isothermal Amplification (LAMP) Assay for the Detection of Bacterial Bovine Mastitis: A Systematic Review"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2955-5638","authenticated-orcid":false,"given":"Tewodros Fentahun","family":"Jember","sequence":"first","affiliation":[{"name":"School of Agricultural Environmental and Veterinary Sciences, Faculty of Science and Health, Charles Sturt University, Wagga Wagga  New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7400-1960","authenticated-orcid":false,"given":"Mark E.","family":"Westman","sequence":"additional","affiliation":[{"name":"Department of Primary Industries and Regional Development Elizabeth Macarthur Agricultural Institute (EMAI)  New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0672-4478","authenticated-orcid":false,"given":"Sameer Dinkar","family":"Pant","sequence":"additional","affiliation":[{"name":"School of Agricultural Environmental and Veterinary Sciences, Faculty of Science and Health, Charles Sturt University, Wagga Wagga  New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9820-5308","authenticated-orcid":false,"given":"Seyed Ali","family":"Ghorashi","sequence":"additional","affiliation":[{"name":"School of Agricultural Environmental and Veterinary Sciences, Faculty of Science and Health, Charles Sturt University, Wagga Wagga  New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2014.0085"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.22092\/ari.2022.358328.2193"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917\u2010016\u20100905\u20103"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2017.05.001"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvfa.2018.07.003"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010023\u201031760\u2010z"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2021.743725"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.14202\/vetworld.2018.562\u2010577"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2021.746755"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1050\u20103862(96)80005\u20102"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2018\u201015306"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12223\u2010017\u20100576\u2010x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250\u2010018\u20101629\u20100"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.NPR.0000469262.22776.1d"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2010.10.006"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40793\u2010016\u20100172\u20108"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2015\u201010073"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1053\/tvjl.2002.0724"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.112797"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0b013e3181a554de"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12223\u2010015\u20100384\u20100"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.5713\/ajas.20.0156"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani10122212"},{"key":"e_1_2_9_25_1","volume-title":"Veterinary Medicine: A Textbook of the Diseases of Cattle, Horses, Sheep, Pigs, and Goats","author":"Constable P. 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