{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T09:49:16Z","timestamp":1791798556306,"version":"4.3.4"},"reference-count":16,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T00:00:00Z","timestamp":1770854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T00:00:00Z","timestamp":1770854400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Probiotics may offer benefits in mitigating bone loss and related conditions. Bone resorption is essential for bone remodelling, particularly during orthodontic tooth movement induced by mechanical forces. This study investigates the effects of\n                      <jats:italic>Bifidobacterium<\/jats:italic>\n                      probiotic supplementation on interleukin\u20106 (IL\u20106) levels and osteoclast counts.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and Materials<\/jats:title>\n                    <jats:p>\n                      This study involved 48 healthy male Wistar rats, randomly assigned to six groups: control groups at 3 days (C3), 7 days (C7) and 14 days (C14), and probiotic groups at 3 days (P3), 7 days (P7) and 14 days (P14). The probiotic group received a daily gavage of\n                      <jats:italic>Bifidobacterium<\/jats:italic>\n                      at a dose of\u00a01.5 \u00d7 10\n                      <jats:sup>8<\/jats:sup>\n                      \u00a0CFU\/g. Orthodontic tooth movement was induced using a coil spring applying a force of 20 g. Osteoclast counts were assessed via light microscopy, while IL\u20106 levels were measured using the ELISA method.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Probiotic supplementation resulted in a significant reduction in IL\u20106 levels and osteoclast counts compared to the control group. Peak IL\u20106 levels and osteoclast counts in the control group were observed on Day 14, whereas the lowest levels were recorded in the probiotic group on the same day.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Despite certain limitations, this study suggests that\n                      <jats:italic>Bifidobacterium animalis<\/jats:italic>\n                      subsp.\n                      <jats:italic>lactis<\/jats:italic>\n                      supplementation effectively reduces IL\u20106 levels and osteoclast counts during orthodontic tooth movement over a 14\u2010day period when compared to controls.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70848","type":"journal-article","created":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T14:46:06Z","timestamp":1770907566000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of\n                    <i>Bifidobacterium<\/i>\n                    Probiotic Supplementation on Interleukin\u20106 Levels and Osteoclast Count in Wistar Rats During Orthodontic Tooth Movement"],"prefix":"10.1002","volume":"12","author":[{"given":"Somayeh","family":"Sarani","sequence":"first","affiliation":[{"name":"Department of Orthodontics, Faculty of Dentistry Zahedan University of Medical Sciences  Zahedan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3440-0198","authenticated-orcid":false,"given":"Marzieh Karimi","family":"Afshar","sequence":"additional","affiliation":[{"name":"Department of Orthodontics, Faculty of Dentistry Kerman University of Medical Sciences  Kerman Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4568-6619","authenticated-orcid":false,"given":"Amir Saeed","family":"Samimi","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Faculty of Veterinary Medicine Shahid Bahonar University of Kerman  Kerman Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4485-5476","authenticated-orcid":false,"given":"Mehrnaz Karimi","family":"Afshar","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics, Faculty of Dentistry Shiraz University of Medical Sciences  Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,12]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"crossref","DOI":"10.1590\/pboci.2020.086","article-title":"Comparison of TNF\u2010\u03b1 and IL\u20101\u03b2 Concentrations in Gingival Crevicular Fluid During Early Alignment Stage of Orthodontic Treatment in Adults and Adolescents","volume":"20","author":"Afshar M. K.","year":"2020","journal-title":"Pesquisa Brasileira em Odontopediatria e Cl\u00ednica Integrada"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2021.105324"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41522-022-00307-x"},{"key":"e_1_2_14_5_1","doi-asserted-by":"publisher","DOI":"10.4103\/jfmpc.jfmpc_204_19"},{"issue":"4","key":"e_1_2_14_6_1","first-page":"367","article-title":"A Comparative Study on the IL\u20108 Expression in Gingival Crevicular Fluid During Early Alignment Stage of Orthodontic Treatment in Adults and Adolescents","volume":"28","author":"Karimi\u2010Afshar M.","year":"2021","journal-title":"Journal of Kerman University of Medical Sciences"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08530"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells12010184"},{"key":"e_1_2_14_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-018-0403-7"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/897639"},{"key":"e_1_2_14_11_1","article-title":"Effect of Probiotics Supplementation on Bone Mineral Content and Bone Mass Density","volume":"2014","author":"Parvaneh K.","year":"2014","journal-title":"Advances in Pharmacological Sciences"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2017.02.017"},{"key":"e_1_2_14_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12903-022-02180-8"},{"key":"e_1_2_14_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2023.09.096"},{"key":"e_1_2_14_15_1","doi-asserted-by":"publisher","DOI":"10.56499\/jppres21.1010_9.4.446"},{"key":"e_1_2_14_16_1","doi-asserted-by":"publisher","DOI":"10.5958\/0974-360X.2021.00262.6"},{"key":"e_1_2_14_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu15234906"}],"container-title":["Veterinary Medicine and Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.70848","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/vms3.70848","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.70848","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T09:25:50Z","timestamp":1791797150000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/vms3.70848"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,12]]},"references-count":16,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1002\/vms3.70848"],"URL":"https:\/\/doi.org\/10.1002\/vms3.70848","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/VMS3.70848\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70848\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70848\/v1\/review2","asserted-by":"object"}]},"ISSN":["2053-1095","2053-1095"],"issn-type":[{"value":"2053-1095","type":"print"},{"value":"2053-1095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,12]]},"assertion":[{"value":"2025-07-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70848"}}