{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T09:49:19Z","timestamp":1791798559538,"version":"4.3.4"},"reference-count":60,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T00:00:00Z","timestamp":1772409600000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      The study evaluates the elemental composition, oral acute toxicity, genotoxicity, and mutagenic potential of\n                      <jats:italic>Heteroctenus junceus<\/jats:italic>\n                      scorpion venom.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The elemental composition was determined by atomic absorption spectrophotometry and HPLC\u2010MS\/MS. The acute oral toxicity test was assessed in mice at 300 and 2000\u00a0mg\/kg. The in vivo micronucleus test was conducted in mice treated with three scorpion venom doses (10, 100, and 1000\u00a0mg\/kg). Meanwhile, the in vitro micronucleus test was conducted in TK6 cells treated with three scorpion venom doses (1.25, 2.5, and 5\u00a0mg\/mL). The Ames test followed the OECD guidelines, and various scorpion venom concentrations (0.16, 0.32, 0.63, 1.25, 2.5, 5\u00a0mg\/mL) were used.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The elemental analysis of scorpion venom reveals the lowest amino acid and elemental composition levels compared to other species. An LD\n                      <jats:sub>50<\/jats:sub>\n                      value greater than 2000\u00a0mg\/kg was obtained in the oral acute toxicity test with transient pathophysiological effects in the liver, lung, and kidney at 300 and 2000\u00a0mg\/kg. The in vitro micronucleus test showed that the negative control and scorpion venom concentrations did not induce micronuclei in TK6 cells. Meanwhile, the in vivo micronucleus test showed an increase in the frequency of micronucleated polychromatic erythrocytes and a dose\u2010dependent decrease in erythrocyte count only at doses higher than 100\u00a0mg\/kg. The Ames reverse mutation test did not show an increase in revertant colonies in the scorpion venom\u2010treated groups compared to the negative control.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      <jats:italic>H. junceus<\/jats:italic>\n                      venom has a low toxicological potential.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70872","type":"journal-article","created":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T04:29:02Z","timestamp":1773030542000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Oral Acute Toxicity and Genotoxicity of\n                    <i>Heteroctenus junceus<\/i>\n                    Scorpion Venom"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7784-7846","authenticated-orcid":false,"given":"Alexis","family":"D\u00edaz\u2010Garc\u00eda","sequence":"first","affiliation":[{"name":"LifEscozul Chile SpA  Santiago de Chile Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2509-4328","authenticated-orcid":false,"given":"Jenny Laura","family":"Ruiz\u2010Fuentes","sequence":"additional","affiliation":[{"name":"LifEscozul Chile SpA  Santiago de Chile Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophia","family":"Mejias Medina","sequence":"additional","affiliation":[{"name":"Qu\u00edmica Industrial y comercial Salimax ltda  Santiago de Chile Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Zapata Guajardo","sequence":"additional","affiliation":[{"name":"Qu\u00edmica Industrial y comercial Salimax ltda  Santiago de Chile Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Ross Skarneo","sequence":"additional","affiliation":[{"name":"Qu\u00edmica Industrial y comercial Salimax ltda  Santiago de Chile Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,2]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-59150-z"},{"key":"e_1_2_11_3_1","first-page":"16","article-title":"Elemental Analysis of Scorpion Venoms","volume":"7","author":"Al\u2010Asmari A. 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