{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T09:49:19Z","timestamp":1791798559082,"version":"4.3.4"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T00:00:00Z","timestamp":1773360000000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      In developing countries, foodborne infections, including those caused by\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                      , pose a significant public health risk.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      This study aimed to isolate\n                      <jats:italic>S. aureus<\/jats:italic>\n                      , assess its antimicrobial resistance and identify factors influencing its prevalence in unloading sites and restaurants in central Gondar, Ethiopia.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>\n                      A cross\u2010sectional study (December 2023\u2013September 2024) was conducted with a total of 301 fish and swab samples were purposively collected. Samples were analysed for\n                      <jats:italic>S. aureus<\/jats:italic>\n                      using culture, Gram staining, biochemical tests and Kirby\u2013Bauer antimicrobial susceptibility testing. Data were analysed using STATA 16 with descriptive statistics at\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Result<\/jats:title>\n                    <jats:p>\n                      From a total of 301 samples were collected from fish, hands and knives at unloading sites, with\n                      <jats:italic>S. aureus<\/jats:italic>\n                      found in 26% of the samples. Of the 201 fish samples, 13% of unfilleted and 39% of filleted fish tested positive. A questionnaire survey revealed that all respondents handled fish with bare hands. Antibiotic testing showed that\n                      <jats:italic>S. aureus<\/jats:italic>\n                      was susceptible to ciprofloxacin (100%) and chloramphenicol (92%) but highly resistant to erythromycin (55%), tetracycline (72%) and penicillin G (87%). Additionally, 57% of isolates were multidrug\u2010resistant.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Over one\u2010fourth of samples were contaminated with\n                      <jats:italic>S. aureus<\/jats:italic>\n                      , mainly due to poor hygiene and bare\u2010hand handling, with high resistance to penicillin and tetracycline observed. Strengthened hygiene practices, routine resistance monitoring and continuous food safety training are recommended.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70880","type":"journal-article","created":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T14:49:43Z","timestamp":1773413383000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Isolation, Antibiogram and Factors Associated With\n                    <i>Staphylococcus aureus<\/i>\n                    From Fish at the Landing Site and Selected Restaurants in Central Gondar, Ethiopia: One Health Approach"],"prefix":"10.1002","volume":"12","author":[{"given":"Misganaw Tezera","family":"Ayele","sequence":"first","affiliation":[{"name":"Department of Pathobiology College of Veterinary Medicine and Animal Sciences, University of Gondar  Gondar Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5804-125X","authenticated-orcid":false,"given":"Asnakew Mulaw","family":"Berihun","sequence":"additional","affiliation":[{"name":"Department of Pathobiology College of Veterinary Medicine and Animal Sciences, University of Gondar  Gondar Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5481-0365","authenticated-orcid":false,"given":"Ambaye Kenubih","family":"Worku","sequence":"additional","affiliation":[{"name":"Department of Pathobiology College of Veterinary Medicine and Animal Sciences, University of Gondar  Gondar Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7806-1389","authenticated-orcid":false,"given":"Mengesha Ayehu","family":"Getnet","sequence":"additional","affiliation":[{"name":"Department of Pathobiology College of Veterinary Medicine and Animal Sciences, University of Gondar  Gondar Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,13]]},"reference":[{"issue":"1","key":"e_1_2_14_2_1","first-page":"71","article-title":"Bacteriological Safety and Antimicrobial Susceptibility of Raw Fish Collected From Lake Tana and Local Fish Markets, Ethiopia","volume":"20","author":"Abebe F.","year":"2016","journal-title":"Ethiopian Veterinary Journal"},{"issue":"4","key":"e_1_2_14_3_1","first-page":"1228","article-title":"Staphylococcus aureus in Raw Fish: Occurrence and Antimicrobial Resistance","volume":"42","author":"Abrahim A.","year":"2011","journal-title":"Brazilian Journal of Microbiology"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.32861\/bmer.53.40.48"},{"key":"e_1_2_14_5_1","unstructured":"Alazer S.2016. \u201cEthiopia: Emphasis on Modern Fish Harvesting Development.\u201dallafrica.com\/stories\/201603100972.html."},{"issue":"2","key":"e_1_2_14_6_1","first-page":"41","article-title":"Prevalence and Antimicrobial Susceptibility Pattern of Staphylococcus aureus Isolated From Cow Milk and Milk Products in and Around Addis Ababa, Ethiopia","volume":"19","author":"Alemayehu D.","year":"2015","journal-title":"Ethiopian Veterinary Journal"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.3329\/jhpn.v26i4.1887"},{"issue":"15","key":"e_1_2_14_8_1","first-page":"610","article-title":"Pathogenicity and Antibiotic Resistance Pattern of Staphylococcus aureus Isolated From Food Handlers in Public Cafeterias, Ethiopia","volume":"11","author":"Asmare K.","year":"2017","journal-title":"African Journal of Microbiology Research"},{"issue":"1","key":"e_1_2_14_9_1","first-page":"34","article-title":"Gender Roles in Fish Marketing and Post\u2010Harvest Handling: Evidence From Lake Tana, Ethiopia","volume":"3","author":"Assefa D.","year":"2018","journal-title":"Journal of Gender, Agriculture and Food Security"},{"issue":"1","key":"e_1_2_14_10_1","first-page":"110","article-title":"Multidrug Resistance and Biofilm Formation Among S. aureus Isolates From Food Handlers in Ethiopia","volume":"22","author":"Beyene B.","year":"2022","journal-title":"BMC Microbiology"},{"key":"e_1_2_14_11_1","unstructured":"Breuil C. andD.Grima.2014.Baseline Report Ethiopia.Smart Fish Programme of the Indian Ocean Commission Fisheries Management FAO Component."},{"issue":"5","key":"e_1_2_14_12_1","first-page":"427","article-title":"Prevalence of Staphylococcus aureus in Fish Samples of Local Domestic Fish Market","volume":"4","author":"Bujjamma P.","year":"2015","journal-title":"International Journal of Current Microbiology and Applied Sciences"},{"key":"e_1_2_14_13_1","first-page":"54","article-title":"Performance Standards for Antimicrobial Susceptibility Testing","volume":"100","author":"CLSI: Clinical and Laboratory Standards Institute","year":"2020","journal-title":"CLSI Supplement"},{"key":"e_1_2_14_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms7120633"},{"issue":"2","key":"e_1_2_14_15_1","first-page":"194","article-title":"Assessment of Hygienic Handling and Processing of Fish in Lake Tana","volume":"5","author":"Dagnew M.","year":"2012","journal-title":"Ethiopian Journal of Environmental Studies and Management"},{"issue":"4","key":"e_1_2_14_16_1","first-page":"180","article-title":"Prevalence and Antibiotic Resistance of S. aureus on Hands and Utensils in Cameroon Fish Markets","volume":"16","author":"Esemu S. N.","year":"2022","journal-title":"African Journal of Microbiological Research"},{"key":"e_1_2_14_17_1","volume-title":"Codex Alimentarius, Food Hygiene Basic Texts","author":"FAO and WHO","year":"2009"},{"key":"e_1_2_14_18_1","volume-title":"State of Fisheries and Aquaculture in the World (2018)","author":"FAO","year":"2018"},{"key":"e_1_2_14_19_1","unstructured":"Gondar City Administration.2015.Structural Plan of Gondar.Gondar City Administration."},{"key":"e_1_2_14_20_1","article-title":"Microbial Quality of Nile Tilapia (Oreochromis niloticus) Fillets and Handling Practices in Fish Retail Outlets in Addis Ababa, Ethiopia","volume":"2020","author":"Haile G.","year":"2020","journal-title":"Journal of Food Quality"},{"issue":"1","key":"e_1_2_14_21_1","first-page":"160","article-title":"Occurrence of S. aureus in Seafood Sold in Libya and Its Resistance Profile","volume":"14","author":"Hesham E. M.","year":"2019","journal-title":"Libyan Journal of Medicine"},{"key":"e_1_2_14_22_1","doi-asserted-by":"publisher","DOI":"10.33762\/bvetr.2014.88123"},{"issue":"1","key":"e_1_2_14_23_1","first-page":"25","article-title":"Assessment of Microbial Contamination of Knives and Cutting Boards Used in Fish Processing in Nigeria","volume":"35","author":"Iyevhobu G. I.","year":"2021","journal-title":"Nigerian Journal of Microbiology"},{"issue":"4","key":"e_1_2_14_24_1","first-page":"247","article-title":"Antimicrobial Resistance in Bacteria From Food of Animal Origin in Ethiopia: A Review","volume":"31","author":"Kibrom A.","year":"2017","journal-title":"Ethiopian Journal of Health Development"},{"key":"e_1_2_14_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866\u2010022\u201002640\u20109"},{"key":"e_1_2_14_26_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-70.12.2764"},{"key":"e_1_2_14_27_1","unstructured":"Mainous M. E. P.Salgado C.Perea andM.Nunez.2006.Assessment of fisheries potential and management strategies in Ethiopia's inland waters.Rome:Food and Agriculture Organization (FAO)."},{"issue":"3","key":"e_1_2_14_28_1","first-page":"130","article-title":"Prevalence and Antimicrobial Resistance of S. aureus Isolated From Fish in Iran","volume":"4","author":"Mehdi H.","year":"2012","journal-title":"Iranian Journal of Microbiology"},{"key":"e_1_2_14_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2020.116421"},{"key":"e_1_2_14_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.onehlt.2021.100323"},{"key":"e_1_2_14_31_1","unstructured":"Oumer M.2021. \u201cAssessment of Bacterial Contamination and Handling Practices of Raw Fish in Bahir Dar Ethiopia.\u201d Unpublished MSc thesis or local journal."},{"key":"e_1_2_14_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph10083634"},{"key":"e_1_2_14_33_1","volume-title":"Veterinary Microbiology Microbial Diseases\u2010Bacterial Causes of Bovine Mastitis","author":"Quinn P. J.","year":"2002"},{"issue":"10","key":"e_1_2_14_34_1","first-page":"1833","article-title":"Prevalence and Characteristics of Staphylococcus aureus in Seafood in Japan","volume":"73","author":"Saito T.","year":"2010","journal-title":"Journal of Food Protection"},{"issue":"1","key":"e_1_2_14_35_1","first-page":"S55","article-title":"Prioritizing Zoonoses for Global Health Capacity Building Themes From One Health Zoonotic Disease Workshops in 7 Countries, 2014\u20132016","volume":"23","author":"Salyer S. J.","year":"2017","journal-title":"Emerging Infectious Diseases"},{"key":"e_1_2_14_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01358-06"},{"issue":"2","key":"e_1_2_14_37_1","first-page":"42","article-title":"Fishery and Gender Roles in Lake Tana: A Case of Male\u2010Dominated Sector","volume":"32","author":"Tefera A.","year":"2010","journal-title":"Ethiopian Journal of Development Research"},{"key":"e_1_2_14_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-020-02024-5"},{"issue":"3","key":"e_1_2_14_39_1","first-page":"250","article-title":"Bacterial Load Assessment of Fresh Fish From Lake Tana and Hygiene Evaluation of Handling Sites","volume":"29","author":"Tessema B.","year":"2020","journal-title":"Journal of Aquatic Food Product Technology"},{"key":"e_1_2_14_40_1","volume-title":"Veterinary Epidemiology","author":"Thrusfield M.","year":"2005"},{"issue":"1","key":"e_1_2_14_41_1","first-page":"48","article-title":"Occurrence of Staphylococcus aureus in Fish and Fishery Products in India","volume":"55","author":"Visnuvinayagam S.","year":"2015","journal-title":"Indian Journal of Microbiology"},{"key":"e_1_2_14_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(05)70295-4"},{"issue":"10","key":"e_1_2_14_43_1","first-page":"915","article-title":"Occurrence of Staphylococcus aureus on Hands and Utensils of Restaurant Workers in South Korea","volume":"19","author":"Yoon Y.","year":"2008","journal-title":"Food Control"}],"container-title":["Veterinary Medicine and Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.70880","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/vms3.70880","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.70880","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T09:26:03Z","timestamp":1791797163000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/vms3.70880"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":42,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1002\/vms3.70880"],"URL":"https:\/\/doi.org\/10.1002\/vms3.70880","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/VMS3.70880\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70880\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70880\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70880\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70880\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70880\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.70880\/v1\/decision1","asserted-by":"object"}]},"ISSN":["2053-1095","2053-1095"],"issn-type":[{"value":"2053-1095","type":"print"},{"value":"2053-1095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"2025-06-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70880"}}